Integration of Complete Plasmids Containing Bont Genes into Chromosomes of Clostridium parabotulinum, Clostridium sporogenes, and Clostridium argentinense

Smith, Theresa J.; Tian, Renmao; Imanian, Behzad; Williamson, Charles H.  D.; Johnson, Shannon L.; Daligault, Hajnalka E.; Schill, Kristin M.

doi:10.3390/toxins13070473

Integration of Complete Plasmids Containing Bont Genes into Chromosomes of Clostridium parabotulinum, Clostridium sporogenes, and Clostridium argentinense

Journal Article · Thu Jul 08 00:00:00 EDT 2021 · Toxins

DOI:https://doi.org/10.3390/toxins13070473· OSTI ID:1815750

Smith, Theresa J. ^[1]; Tian, Renmao ^[2]; ^[3]; Williamson, Charles H. D. ^[4]; ^[5]; Daligault, Hajnalka E. ^[5]; ^[6]

Northern Arizona Univ., Flagstaff, AZ (United States). Pathogen and Microbiome Inst.; OSTI
Illinois Inst. of Technology, Bedford Park, IL (United States). Inst. for Food Safety and Health
Illinois Inst. of Technology, Bedford Park, IL (United States). Inst. for Food Safety and Health; Illinois Inst. of Technology, Bedford Park, IL (United States). Food Science and Nutrition
Northern Arizona Univ., Flagstaff, AZ (United States). Pathogen and Microbiome Inst.
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Food and Drug Administration, Bedford Park, IL (United States). Center for Food Safety and Applied Nutrition

At least 40 toxin subtypes of botulinum neurotoxins (BoNTs), a heterogenous group of bacterial proteins, are produced by seven different clostridial species. A key factor that drives the diversity of neurotoxigenic clostridia is the association of bont gene clusters with various genomic locations including plasmids, phages and the chromosome. Analysis of Clostridium sporogenes BoNT/B1 strain CDC 1632, C. argentinense BoNT/G strain CDC 2741, and Clostridium parabotulinum BoNT/B1 strain DFPST0006 genomes revealed bont gene clusters within plasmid-like sequences within the chromosome or nested in large contigs, with no evidence of extrachromosomal elements. A nucleotide sequence (255,474 bp) identified in CDC 1632 shared 99.5% identity (88% coverage) with bont/B1-containing plasmid pNPD7 of C. sporogenes CDC 67071; CDC 2741 contig AYSO01000020 (1.1 MB) contained a ~140 kb region which shared 99.99% identity (100% coverage) with plasmid pRSJ17_1 of C. argentinense BoNT/G strain 89G; and DFPST0006 contig JACBDK0100002 (573 kb) contained a region that shared 100% identity (99%) coverage with the bont/B1-containing plasmid pCLD of C. parabotulinum Okra. This is the first report of full-length plasmid DNA-carrying complete neurotoxin gene clusters integrated in three distinct neurotoxigenic species: C. parabotulinum, C. sporogenes and C. argentinense.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: US Food and Drug Administration (FDA); USDOE Office of Science (SC)

Grant/Contract Number:: 89233218CNA000001

OSTI ID:: 1815750

Journal Information:: Toxins, Journal Name: Toxins Journal Issue: 7 Vol. 13; ISSN 2072-6651; ISSN TOXIB7

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Integration of Complete Plasmids Containing Bont Genes into Chromosomes of Clostridium parabotulinum, Clostridium sporogenes, and Clostridium argentinense

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