Hypochromicity in red blood cells: an experimental and theoretical investigation

Nonoyama, Akihisa; Garcia-Lopez, Alicia; Garcia-Rubio, Luis H.; Leparc, German F.; Potter, Robert L.

doi:10.1364/boe.2.002126

Hypochromicity in red blood cells: an experimental and theoretical investigation

Journal Article · Fri Jul 01 00:00:00 EDT 2011 · Biomedical Optics Express

DOI:https://doi.org/10.1364/boe.2.002126· OSTI ID:1627120

Nonoyama, Akihisa ^[1]; Garcia-Lopez, Alicia ^[2]; Garcia-Rubio, Luis H. ^[3]; Leparc, German F. ^[4]; Potter, Robert L. ^[5]

University of South Florida, Tampa, FL (United States); DOE/OSTI
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Claro Scientific, St. Petersburg, FL (United States); Florida Blood Services, St. Petersburg, FL (United States)
Florida Blood Services, St. Petersburg, FL (United States)
University of South Florida, Tampa, FL (United States)

Multiwavelength UV-visible transmission spectrophotometry is a useful tool for the examination of micron-size particle suspensions in the context of particle size and chemical composition. This paper reports the reliability of this method to characterize the spectra of purified red blood cells both in their physiological state and with modified hemoglobin content. Previous studies have suggested the contribution of hypochromism on the particle spectra caused by the close electronic interaction of the encapsulated chromophores. Our research shows, however, that this perceived hypochromism can be accounted for by considering two important issues: the acceptance angle of the instrument and the combined scattering and absorption effect of light on the particles. In order to establish these ideas, spectral analysis was performed on purified and modified red cells where the latter was accomplished with a modified hypotonic shock protocol that altered the hemoglobin concentration within the cells. Moreover, the Mie theory was used to successfully simulate the spectral features and trends of the red cells. With this combination of experimental and theoretical exploration, definition of hypochromism has been extended to two subcategories.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1627120

Journal Information:: Biomedical Optics Express, Journal Name: Biomedical Optics Express Journal Issue: 8 Vol. 2; ISSN 2156-7085

Publisher:: Optical Society of America (OSA)Copyright Statement

Country of Publication:: United States

Language:: English

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