Relationship between red blood cell uptake and methemoglobin production by nitrobenzene and dinitrobenzene in vitro

Journal Article · Mon Jan 14 04:00:00 EST 1985 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(85)90090-6· OSTI ID:5869409

Goldstein, R S; Rickert, D E

Nitrobenzene increases methemoglobin formation when incubated with native hemoglobin but not when incubated with red blood cell suspensions. These experiments were designed to determine if transport of nitrobenzene across the red blood cell membrane is a limiting factor for methemoglobin production by red blood cell suspensions. Incubation of (/sup 14/C)-m-, o- or p-dinitrobenzene, but not mononitrobenzene, with red blood cell suspensions caused a time-dependent increase in methemoglobin. All three dinitrobenzenes and mononitrobenzene crossed the red blood cell membrane and accumulated in the erythrocytes after only 1 min of incubation. Incubation of mononitrobenzene with hemolysates did not result in methemoglobin production. Incubation of red blood cells with the dinitrobenzenes or mononitrobenzene for 1 and 10 min at 4/sup 0/C did not influence red blood cell uptake of the nitrobenzenes, suggesting that these compounds do not enter the red blood cell by an active process. Dinitrobenzene-induced methemoglobin production was markedly inhibited at 4/sup 0/C, and may be a result of decreased interaction with hemoglobin and/or decreased metabolism to reactive intermediates which mediate methemoglobin production. These data indicate that red blood cell transport of nitrobenzene is not the limiting factor in methemoglobin production in vitro. 7 references, 3 tables.

• Research Organization: Chemical Industry Inst. of Toxicology, Research Triangle Park, NC
• OSTI ID: 5869409
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 36:2; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DATA
ERYTHROCYTES
EXPERIMENTAL DATA
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IN VITRO
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
METABOLISM
METHEMOGLOBIN
NITRO COMPOUNDS
NITROBENZENE
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RATS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES