Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae

Li, Xin; Chomvong, Kulika; Yu, Vivian Yaci; Liang, Julie M.; Lin, Yuping; Cate, Jamie H. D.

doi:10.1186/s13068-015-0303-2

Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae

Journal Article · Sun Aug 16 00:00:00 EDT 2015 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-015-0303-2· OSTI ID:1626965

Li, Xin ^[1]; Chomvong, Kulika ^[2]; Yu, Vivian Yaci ^[3]; Liang, Julie M. ^[4]; Lin, Yuping ^[3]; Cate, Jamie H. D. ^[5]

Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; DOE/OSTI
Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division

Background: Economical production of fuels and chemicals from plant biomass requires the efficient use of sugars derived from the plant cell wall. Neurospora crassa, a model lignocellulosic degrading fungus, is capable of breaking down the complex structure of the plant cell wall. In addition to cellulases and hemicellulases, N. crassa secretes lytic polysaccharide monooxygenases (LPMOs), which cleave cellulose by generating oxidized sugars—particularly aldonic acids. However, the strategies N. crassa employs to utilize these sugars are unknown. Results: We identified an aldonic acid utilization pathway in N. crassa, comprised of an extracellular hydrolase (NCU08755), cellobionic acid transporter (CBT-1, NCU05853) and cellobionic acid phosphorylase (CAP, NCU09425). Extracellular cellobionic acid could be imported directly by CBT-1 or cleaved to gluconic acid and glucose by a β-glucosidase (NCU08755) outside the cells. Intracellular cellobionic acid was further cleaved to glucose 1-phosphate and gluconic acid by CAP. However, it remains unclear how N. crassa utilizes extracellular gluconic acid. The aldonic acid pathway was successfully implemented in Saccharomyces cerevisiae when N. crassa gluconokinase was coexpressed, resulting in cellobionic acid consumption in both aerobic and anaerobic conditions. Conclusions: We successfully identified a branched aldonic acid utilization pathway in N. crassa and transferred its essential components into S. cerevisiae, a robust industrial microorganism.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1626965

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 8; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
AA9
Aldonic acid
Biofuels
Biotechnology & Applied Microbiology
Cellobionic acid
Energy & Fuels
LPMO
Metabolic engineering
Phosphorylase
Transporter
β-glucosidase

Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae Li, Xin; Chomvong, Kulika; Yu, Vivian https://doi.org/10.6084/m9.figshare.c.3609959 The current record is supplemented by this collection	collection	January 2015
Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae Li, Xin; Chomvong, Kulika; Yu, Vivian https://doi.org/10.6084/m9.figshare.c.3609959.v1 The current record is supplemented by this collection	collection	January 2015
Additional file 1. of Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae Li, Xin; Chomvong, Kulika; Yu, Vivian https://doi.org/10.6084/m9.figshare.c.3609959_d1 The current record is supplemented by this text	text	January 2015
Additional file 1. of Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae Li, Xin; Chomvong, Kulika; Yu, Vivian https://doi.org/10.6084/m9.figshare.c.3609959_d1.v1 The current record is supplemented by this text	text	January 2015

Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae

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