The role of lignin in the conversion of wheat straw to cellobionic acid by Neurospora crassa HL10

Zhou, Man; Tao, Lizhi; Russell, Peter; Britt, R. David; Kasuga, Takao; Lü, Xin; Fan, Zhiliang

doi:10.1016/j.indcrop.2022.115650

The role of lignin in the conversion of wheat straw to cellobionic acid by Neurospora crassa HL10

Journal Article · Tue Sep 20 00:00:00 EDT 2022 · Industrial Crops and Products

DOI:https://doi.org/10.1016/j.indcrop.2022.115650· OSTI ID:2419726

Zhou, Man ^[1]; Tao, Lizhi ^[2]; Russell, Peter ^[2]; Britt, R. David ^[2]; Kasuga, Takao ^[3]; Lü, Xin ^[4]; Fan, Zhiliang ^[2]

Univ. of California, Davis, CA (United States); Northwest A & F University (China); OSTI
Univ. of California, Davis, CA (United States)
Univ. of California, Davis, CA (United States); US Dept. of Agriculture (USDA), Davis, CA (United States). Agricultural Research Service (ARS)
Northwest A & F University (China)

An engineered Neurospora crassa strain (HL10) naturally produces cellulases, cellobiose dehydrogenase (CDH), and heterologously expresses laccase. It can convert Avicel (cellulose) to cellobionic acid (cellobionate) without any enzyme addition at high yields. However, the addition of a catalytic amount of artificial redox mediators was required to achieve a high yield conversion. When a lignocellulosic substrate like wheat straw was used as the carbon source, it was found that adding exogenous artificial redox mediators did not improve cellobionate yield. We hypothesized that lignin and lignin degradation products were able to serve as redox mediators for the CDH-laccase conversion system. Experiments were designed to prove the hypothesis. When N. crassa HL10 was grown on Avicel, the addition of enzyme hydrolyzed lignin (EHL) can achieve a similar level of cellobionate production as with ABTS addition. The addition of EHL can increase the cellobionate production rate in the CDH-laccase bi-enzyme system using cellobiose as the substrate. The formation of lignin radicals, and the quenching of lignin radicals by reduced CDH, were verified by an electron paramagnetic resonance (EPR) experiment, providing further evidence that lignin radicals can serve as the electron acceptor of reduced CDH. Hence, lignocellulosic biomass is a self-sufficient substrate for the production of cellobionate. The conversion of the lignocellulosic substrate to cellobionate using N. crassa HL10 can be achieved without any exogenous enzyme addition or redox mediator addition.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: SC0007203

OSTI ID:: 2419726

Journal Information:: Industrial Crops and Products, Journal Name: Industrial Crops and Products Journal Issue: Part A Vol. 188; ISSN 0926-6690

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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The role of lignin in the conversion of wheat straw to cellobionic acid by Neurospora crassa HL10

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