Light-dependent roles of the G-protein α subunit GNA1 of Hypocrea jecorina (anamorph Trichoderma reesei)

Seibel, Christian; Gremel, Gabriela; do Nascimento Silva, Roberto; Schuster, André; Kubicek, Christian P.; Schmoll, Monika

doi:10.1186/1741-7007-7-58

Light-dependent roles of the G-protein α subunit GNA1 of Hypocrea jecorina (anamorph Trichoderma reesei)

Journal Article · Thu Sep 03 00:00:00 EDT 2009 · BMC Biology

DOI:https://doi.org/10.1186/1741-7007-7-58· OSTI ID:1626601

Seibel, Christian ^[1]; Gremel, Gabriela ^[2]; do Nascimento Silva, Roberto ^[2]; Schuster, André ^[2]; Kubicek, Christian P. ^[2]; Schmoll, Monika ^[2]

Vienna Univ. of Technology (Austria). Inst. of Chemical Engineering. Research Area Gene Technology and Applied Biochemistry; DOE/OSTI
Vienna Univ. of Technology (Austria). Inst. of Chemical Engineering. Research Area Gene Technology and Applied Biochemistry

Background: The filamentous ascomycete Hypocrea jecorina (anamorph Trichoderma reesei) is primarily known for its efficient enzymatic machinery that it utilizes to decompose cellulosic substrates. Nevertheless, the nature and transmission of the signals initiating and modulating this machinery are largely unknown. Heterotrimeric G-protein signaling represents one of the best studied signal transduction pathways in fungi. Results: Analysis of the regulatory targets of the G-protein α subunit GNA1 in H. jecorina revealed a carbon source and light-dependent role in signal transduction. Deletion of gna1 led to significantly decreased biomass formation in darkness in submersed culture but had only minor effects on morphology and hyphal apical extension rates on solid medium. Cellulase gene transcription was abolished in Δgna1 on cellulose in light and enhanced in darkness. However, analysis of strains expressing a constitutively activated GNA1 revealed that GNA1 does not transmit the essential inducing signal. Instead, it relates a modulating signal with light-dependent significance, since induction still required the presence of an inducer. We show that regulation of transcription and activity of GNA1 involves a carbon source-dependent feedback cycle. Additionally we found a function of GNA1 in hydrophobin regulation as well as effects on conidiation and tolerance of osmotic and oxidative stress. Conclusion: We conclude that GNA1 transmits a signal the physiological relevance of which is dependent on both the carbon source as well as the light status. The widespread consequences of mutations in GNA1 indicate a broad function of this Gα subunit in appropriation of intracellular resources to environmental (especially nutritional) conditions.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: Austrian Science Foundation; USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1626601

Journal Information:: BMC Biology, Journal Name: BMC Biology Journal Issue: 1 Vol. 7; ISSN 1741-7007

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Regulation of plant cell wall degradation by light in Trichoderma Schmoll, Monika Fungal Biology and Biotechnology, Vol. 5, Issue 1 https://doi.org/10.1186/s40694-018-0052-7	journal	April 2018
The role of PKAc1 in gene regulation and trichodimerol production in Trichoderma reesei Hinterdobler, Wolfgang; Schuster, André; Tisch, Doris Fungal Biology and Biotechnology, Vol. 6, Issue 1 https://doi.org/10.1186/s40694-019-0075-8	journal	September 2019
Strain improvement and process optimization of Trichderma reesei Rut C30 for enhanced cellulase production Wu, Gaihong; He, Ronglin; Jia, Wendi Biofuels, Vol. 2, Issue 5 https://doi.org/10.4155/bfs.11.124	journal	September 2011

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