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Identification of the Major Expressed S-Layer and Cell Surface-Layer-Related Proteins in the Model Methanogenic Archaea: Methanosarcina barkeri Fusaro and Methanosarcina acetivorans C2A

Journal Article · · Archaea
DOI:https://doi.org/10.1155/2012/873589· OSTI ID:1626206
 [1];  [2];  [3];  [4];  [5];  [6]
  1. Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics; Univ. of California, Los Angeles, CA (United States). UCLA-DOE Inst. for Genomics and Proteomics; DOE/OSTI
  2. Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry; Boston Univ., MA (United States). School of Medicine. Mass Spectrometry Resource
  3. Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics; OPX Biotechnologies, Inc., Boulder, CO (United States). Research Division
  4. Univ. of California, Los Angeles, CA (United States). UCLA-DOE Inst. for Genomics and Proteomics; Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry; Univ. of California, Los Angeles, CA (United States). Dept. of Biological Chemistry
  5. Univ. of California, Los Angeles, CA (United States). UCLA-DOE Inst. for Genomics and Proteomics; Univ. of California, Los Angeles, CA (United States). Dept. of Biological Chemistry
  6. Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics; Univ. of California, Los Angeles, CA (United States). UCLA-DOE Inst. for Genomics and Proteomics
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Many archaeal cell envelopes contain a protein coat or sheath composed of one or more surface exposed proteins. These surface layer (S-layer) proteins contribute structural integrity and protect the lipid membrane from environmental challenges. To explore the species diversity of these layers in the Methanosarcinaceae, the major S-layer protein in Methanosarcina barkeri strain Fusaro was identified using proteomics. The Mbar_A1758 gene product was present in multiple forms with apparent sizes of 130, 120, and 100 kDa, consistent with post-translational modifications including signal peptide excision and protein glycosylation. A protein with features related to the surface layer proteins found in Methanosarcina acetivorans C2A and Methanosarcina mazei Goel was identified in the M. barkeri genome. These data reveal a distinct conserved protein signature with features and implied cell surface architecture in the Methanosarcinaceae that is absent in other archaea. Paralogous gene expression patterns in two Methanosarcina species revealed abundant expression of a single S-layer paralog in each strain. Respective promoter elements were identified and shown to be conserved in mRNA coding and upstream untranslated regions. Prior M. acetivorans genome annotations assigned S-layer or surface layer associated roles of eighty genes: however, of 68 examined none was significantly expressed relative to the experimentally determined S-layer gene.
Research Organization:
Univ. of California, Los Angeles, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
FG02-08ER64689
OSTI ID:
1626206
Journal Information:
Archaea, Journal Name: Archaea Vol. 2012; ISSN 1472-3646
Publisher:
HindawiCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (4)

Mining proteomic data to expose protein modifications in Methanosarcina mazei strain Gö1 journal March 2015
Emerging facets of prokaryotic glycosylation journal August 2016
N-Linked Glycosylation in Archaea: a Structural, Functional, and Genetic Analysis journal May 2014
The complete genome sequence of the rumen methanogen Methanosarcina barkeri CM1 journal August 2015

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