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MreA Functions in the Global Regulation of Methanogenic Pathways in Methanosarcina acetivorans

Journal Article · · mBio (Online)
 [1];  [2];  [2];  [2]
  1. Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemsitry and Molecular Biology; DOE/OSTI
  2. Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemsitry and Molecular Biology
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Results are presented supporting a regulatory role for the product of the MA3302 gene locus (designated MreA) previously annotated as a hypothetical protein in the methanogenic species Methanosarcina acetivorans of the domain Archaea. Sequence analysis of MreA revealed identity to the TrmB family of transcription factors, albeit the sequence is lacking the sensor domain analogous to TrmBL2, abundant in nonmethanogenic species of the domain Archaea. Transcription of mreA was highly upregulated during growth on acetate versus methylotrophic substrates, and an mreA deletion (ΔmreA) strain was impaired for growth with acetate in contrast to normal growth with methylotrophic substrates. Transcriptional profiling of acetate-grown cells identified 280 genes with altered expression in the ΔmreA strain versus the wild-type strain. Expression of genes unique to the acetate pathway decreased whereas expression of genes unique to methylotrophic metabolism increased in the ΔmreA strain relative to the wild type, results indicative of a dual role for MreA in either the direct or indirect activation of acetate-specific genes and repression of methylotrophic-specific genes. Gel shift experiments revealed specific binding of MreA to promoter regions of regulated genes. Homologs of MreA were identified in M. acetivorans and other Methanosarcina species for which expression patterns indicate roles in regulating methylotrophic pathways.
Research Organization:
Pennsylvania State Univ., University Park, PA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
FG02-95ER20198
OSTI ID:
1626092
Journal Information:
mBio (Online), Journal Name: mBio (Online) Journal Issue: 4 Vol. 3; ISSN 2150-7511
Publisher:
American Society for Microbiology (ASM)Copyright Statement
Country of Publication:
United States
Language:
English

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Cited By (5)

The TrmB family: a versatile group of transcriptional regulators in Archaea journal August 2014
Contribution of Transcriptomics to Systems-Level Understanding of Methanogenic Archaea journal January 2013
Genome-wide gene expression and RNA half-life measurements allow predictions of regulation and metabolic behavior in Methanosarcina acetivorans journal November 2016
Genetic, Biochemical, and Molecular Characterization of Methanosarcina barkeri Mutants Lacking Three Distinct Classes of Hydrogenase journal July 2018
Towards a Computational Model of a Methane Producing Archaeum journal January 2014

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59 BASIC BIOLOGICAL SCIENCES
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