Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids

Cary, Robert B.

Title: Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids

Patent · Tue Oct 29 00:00:00 EDT 2019

OSTI ID:1600229

Cary, Robert B.

The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10 microliters, significantly reducing assay costs and ameliorating reagent storage logistics. Additionally, the miniaturization of lateral flow opens the door to highly multiplexed assays, allowing many proteins or nucleic acids to be detected in a single assay.

View Patent

Cite

Export

Save

Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC52-06NA25396

Assignee:: Triad National Security, LLC (Los Alamos, NM)

Patent Number(s):: 10,458,978

Application Number:: 15/431,705

OSTI ID:: 1600229

Resource Relation:: Patent File Date: 02/13/2017

Country of Publication:: United States

Language:: English

References (294)

Lateral flow methods and devices for detection of nucleic acid binding proteins Nilsen, Thor W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070015166 US Patent Application 11/181260; 20070015166	patent-application	January 2007
Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus Grainge, I. Nucleic Acids Research, Vol. 31, Issue 16 https://doi.org/10.1093/nar/gkg662	journal	August 2003
Methods and compositions for amplification of RNA sequences Kurn, Nurith https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050014192 US Patent Application 10/934890; 20050014192	patent-application	January 2005
Shape matters Buhro, William E.; Colvin, Vicki L. Nature Materials, Vol. 2, Issue 3 https://doi.org/10.1038/nmat844	journal	March 2003
Rapid and Sensitive Colorimetric Detection of Xanthomonas axonopodis pv. citri by Immunocapture and a Nested-Polymerase Chain Reaction Assay Hartung, John Phytopathology, Vol. 86, Issue 1 https://doi.org/10.1094/Phyto-86-95	journal	January 1996
Pharmaceutical composition containing a stabilised mRNA optimised for translation in its coding regions Von Der Mulbe, Florian; Hoerr, Ingmar; , Steve Pascolo https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050032730 US Patent Application 10/729830; 20050032730	patent-application	February 2005
Miniaturized Lateral Flow Device for Rapid and Sensitive Detection of Proteins or Nucleic Acids Cary, Robert B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090047673 US Patent Application 11/894910; 20090047673	patent-application	February 2009
A one-step coupled amplification and oligonucleotide ligation procedure for multiplex genetic typing. Eggerding, F. A. Genome Research, Vol. 4, Issue 6 https://doi.org/10.1101/gr.4.6.337	journal	June 1995
Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR Cubero, J.; Graham, J. H. Applied and Environmental Microbiology, Vol. 68, Issue 3 https://doi.org/10.1128/AEM.68.3.1257-1264.2002	journal	March 2002
Simultaneous Visual Detection of Multiple Viral Amplicons by Dipstick Assay Dineva, M. A.; Candotti, D.; Fletcher-Brown, F. Journal of Clinical Microbiology, Vol. 43, Issue 8 https://doi.org/10.1128/JCM.43.8.4015-4021.2005	journal	August 2005
Nucleic acid detection system Jung, Jaean; Choi, Young Ho; Kim, Youngsun https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050227275 US Patent Application 11/102001; 20050227275	patent-application	October 2005
Signal amplification through nucleotide extension and excision on a dendritic DNA platform Capaldi, S. Nucleic Acids Research, Vol. 28, Issue 7 https://doi.org/10.1093/nar/28.7.e21	journal	April 2000
Rapid, Sensitive, and Specific Lateral-Flow Immunochromatographic Device To Measure Anti-Anthrax Protective Antigen Immunoglobulin G in Serum and Whole Blood Biagini, R. E.; Sammons, D. L.; Smith, J. P. Clinical and Vaccine Immunology, Vol. 13, Issue 5 https://doi.org/10.1128/CVI.13.5.541-546.2006	journal	May 2006
Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans Brlansky, R. H.; Damsteegt, V. D.; Hartung, J. S. Plant Disease, Vol. 86, Issue 11 https://doi.org/10.1094/PDIS.2002.86.11.1237	journal	November 2002
Helicase-dependent isothermal DNA amplification Vincent, Myriam; Xu, Yan; Kong, Huimin EMBO reports, Vol. 5, Issue 8 https://doi.org/10.1038/sj.embor.7400200	journal	August 2004
Asymmetrically branched polymer conjugates and microarray assays Yin, Ray; Qin, Dujie; Pan, Jing https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060041058 US Patent Application 10/993474; 20060041058	patent-application	February 2006
Genetic Diversity of Pierce's Disease Strains and Other Pathotypes of Xylella fastidiosa Hendson, M.; Purcell, A. H.; Chen, D. Applied and Environmental Microbiology, Vol. 67, Issue 2 https://doi.org/10.1128/AEM.67.2.895-903.2001	journal	February 2001
Mesothelium antigen and methods and kits for targeting it Pastan, Ira H.; Chang, Kai https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6083502 US Patent Document 6,083,502	patent	July 2000
Method and Apparatus for Amplification of Nucleic Acid Sequences by Using Thermal Convection Hwang, Hyun Jin; Kim, Jeong Hee; Jeong, Kyunghoon https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040152122 US Patent Application 10/801342; 20040152122	patent-application	August 2004
Microwave Assisted PCR Amplification of DNA Collins, Michael J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070231798 US Patent Application 11/278286; 20070231798	patent-application	October 2007
Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports Guo, Zhen; Guilfoyle, Richard A.; Thiel, Andrew J. Nucleic Acids Research, Vol. 22, Issue 24 https://doi.org/10.1093/nar/22.24.5456	journal	January 1994
Two step lateral flow assay methods and devices Rundstrom, Gerd; Matsson, Per https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070020768 US Patent Application 11/438882; 20070020768	patent-application	January 2007
A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits Baeumner, Antje J.; Pretz, Jennifer; Fang, Shirley Analytical Chemistry, Vol. 76, Issue 4 https://doi.org/10.1021/ac034945l	journal	February 2004
Reversible flow chromatographic binding assay Clark, Scott M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6007999 US Patent Document 6,007,999	patent	December 1999
Assessment of the sensitivity and specificity of oligonucleotide (50mer) microarrays Kane, M. D. Nucleic Acids Research, Vol. 28, Issue 22 https://doi.org/10.1093/nar/28.22.4552	journal	November 2000
Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase O'Donnell, Michael https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6555349 US Patent Document 6,555,349	patent	April 2003
Populations of Xylella fastidiosa in Plants Required for Transmission by an Efficient Vector Hill, B. L.; Purcell, A. H. Phytopathology, Vol. 87, Issue 12 https://doi.org/10.1094/PHYTO.1997.87.12.1197	journal	December 1997
Gravity-driven micropump Yao, Nan-Kuang; Wu, Jhy-Wen https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8173078 US Patent Document 8,173,078	patent	May 2012
Pumpless microfluidics Weigl, Bernhard; Williams, Clinton L.; Hayenga, Jon W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6743399 US Patent Document 6,743,399	patent	June 2004
Isothermal reactions for the amplification of oligonucleotides Van Ness, J.; Van Ness, L. K.; Galas, D. J. Proceedings of the National Academy of Sciences, Vol. 100, Issue 8, p. 4504-4509 https://doi.org/10.1073/pnas.0730811100	journal	April 2003
Integrated biochip system for sample preparation and analysis Cheng, Jing; Wang, Xiaobo; Wu, Lei https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020076825 US Patent Application 09/973629; 20020076825	patent-application	June 2002
Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum Fukuta, Shiro; Ohishi, Kazushi; Yoshida, Keiko Journal of Virological Methods, Vol. 121, Issue 1, p. 49-55 https://doi.org/10.1016/j.jviromet.2004.05.016	journal	October 2004
Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Guatelli, J. C.; Whitfield, K. M.; Kwoh, D. Y. Proceedings of the National Academy of Sciences, Vol. 87, Issue 5, p. 1874-1878 https://doi.org/10.1073/pnas.87.5.1874	journal	March 1990
A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection Wetzel, T.; Candresse, T.; Macquaire, G. Journal of Virological Methods, Vol. 39, Issue 1-2, p. 27-37 https://doi.org/10.1016/0166-0934(92)90122-T	journal	September 1992
Nanoparticle Probes with Raman Spectroscopic Fingerprints for Analyte Detection Mirkin, Chad A.; Cao, Yunwei; Jin, Rongchao https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040086897 US Patent Application 10/431341; 20040086897	patent-application	May 2004
Capillary flow liquid transfer device having waste reception area Bunce, Roger A.; Starsmore, Stephen J.; Thorpe, Gary H. G. H. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5618494 US Patent Document 5,618,494	patent	April 1997
Helicase structure and mechanism Caruthers, Jonathan M.; McKay, David B. Current Opinion in Structural Biology, Vol. 12, Issue 1, p. 123-133 https://doi.org/10.1016/S0959-440X(02)00298-1	journal	February 2002
Fluorescent Amplified Fragment Length Polymorphism Analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis Isolates Hill, K. K.; Ticknor, L. O.; Okinaka, R. T. Applied and Environmental Microbiology, Vol. 70, Issue 2 https://doi.org/10.1128/AEM.70.2.1068-1080.2004	journal	February 2004
Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture Jacobsen, N. Nucleic Acids Research, Vol. 32, Issue 7 https://doi.org/10.1093/nar/gnh056	journal	April 2004
Potent and nontoxic antisense oligonucleotides containing locked nucleic acids Wahlestedt, C.; Salmi, P.; Good, L. Proceedings of the National Academy of Sciences, Vol. 97, Issue 10 https://doi.org/10.1073/pnas.97.10.5633	journal	May 2000
Multistep Reaction Lateral Flow Capillary Device Alajem, Sara; Reinhartz, Avraham https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080145835 US Patent Application 11/883329; 20080145835	patent-application	June 2008
Lateral flow system for nucleic acid detection Gerdes, John C.; Mondesire, Roy R.; Hansen, Lara A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040110167 US Patent Application 10/413433; 20040110167	patent-application	June 2004
Acquisition and Retention of Xylella fastidiosa by an Efficient Vector, Graphocephala atropunctata Hill, B. Phytopathology, Vol. 85, Issue 2 https://doi.org/10.1094/Phyto-85-209	journal	January 1995
Microarray technology – an intellectual property retrospective Rouse, Richard; Hardiman, Gary Pharmacogenomics, Vol. 4, Issue 5 https://doi.org/10.1517/phgs.4.5.623.23792	journal	September 2003
Self-Contained Microfluidic Biochip and Apparatus Ho, Winston Z. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050221281 US Patent Application 11/117908; 20050221281	patent-application	October 2005
DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis Jensen, Michael A.; Fukushima, Marilyn; Davis, Ronald W. PLoS ONE, Vol. 5, Issue 6 https://doi.org/10.1371/journal.pone.0011024	journal	June 2010
Fidelity of DNA polymerases in DNA amplification. Keohavong, P.; Thilly, W. G. Proceedings of the National Academy of Sciences, Vol. 86, Issue 23 https://doi.org/10.1073/pnas.86.23.9253	journal	December 1989
Microfluidic Flow Monitoring Device Rossier, Joel Stephane; Morier, Patrick; Reymond, Frederic https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070039835 US Patent Application 10/571986; 20070039835	patent-application	February 2007
Design, biochemical, biophysical and biological properties of cooperative antisense oligonucleotides Kandimalla, Ekambar R.; Manning, Adrienne; Lathan, Christopher Nucleic Acids Research, Vol. 23, Issue 17 https://doi.org/10.1093/nar/23.17.3578	journal	January 1995
Multiplex diagnostic platforms for detection of biothreat agents Cirino, Nick M.; Musser, Kimberlee A.; Egan, Christina Expert Review of Molecular Diagnostics, Vol. 4, Issue 6 https://doi.org/10.1586/14737159.4.6.841	journal	November 2004
Recombinant fragment assay for gene targetting based on the polymerase chain reaction Kim, H. -S.; Smithies, O. Nucleic Acids Research, Vol. 16, Issue 18 https://doi.org/10.1093/nar/16.18.8887	journal	September 1988
Printed fluid transport devices Alcock, Susan; White, Stephen F.; Turner, Anthony L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5736188 US Patent Document 5,736,188	patent	April 1998
Autonomous Microfluidic Apparatus Wu, Jhy-Wen; Yang, Hung-Jen; Yao, Nan-Kuang https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090053106 US Patent Application 12/007931; 20090053106	patent-application	February 2009
Nanocrystal-Based Lateral Flow Microarrays and Low-Voltage Signal Detection Systems Cary, Robert B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20110160090 US Patent Application 12/991418; 20110160090	patent-application	June 2011
Identification of A/H5N1 Influenza Viruses Using a Single Gene Diagnostic Microarray Dawson, Erica D.; Moore, Chad L.; Dankbar, Daniela M. Analytical Chemistry, Vol. 79, Issue 1 https://doi.org/10.1021/ac061920o	journal	January 2007
Rapid Solid-Phase Immunoassay for Detection of Methicillin-Resistant Staphylococcus aureus Using Cycling Probe Technology Fong, Whalley K.; Modrusan, Zora; Mcnevin, John P. Journal of Clinical Microbiology, Vol. 38, Issue 7 https://doi.org/10.1128/JCM.38.7.2525-2529.2000	journal	January 2000
Formamide can dramatically improve the specificity of PCR Sarkar, Gobinda; Kapelner, Stephen; Sommer, Steve S. Nucleic Acids Research, Vol. 18, Issue 24 https://doi.org/10.1093/nar/18.24.7465	journal	January 1990
Are We Ready for Pandemic Influenza? Webby, R. J. Science, Vol. 302, Issue 5650 https://doi.org/10.1126/science.1090350	journal	November 2003
Chip PCR. I. Surface passivation of microfabricated silicon-glass chips for PCR Shoffner, M. Nucleic Acids Research, Vol. 24, Issue 2 https://doi.org/10.1093/nar/24.2.375	journal	January 1996
Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization Edwards, Katie A.; Baeumner, Antje J. Analytical and Bioanalytical Chemistry, Vol. 386, Issue 5 https://doi.org/10.1007/s00216-006-0705-x	journal	August 2006
LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition Singh, Sanjay K.; Koshkin, Alexei A.; Wengel, Jesper Chemical Communications, Issue 4 https://doi.org/10.1039/a708608c	journal	January 1998
Diagnostic marker for tumor hypoxia and prognosis Giaccia, Amato J.; Le, Quynh Thu; Denko, Nicholas C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030044862 US Patent Application 10/175440; 20030044862	patent-application	March 2003
Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control Cary, Robert B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9207236 US Patent Document 9,207,236	patent	December 2015
Unitary biochip providing sample-in to results-out processing and methods of manufacture Selden, Richard F.; Tan, Eugene https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9354199 US Patent Document 9,354,199	patent	May 2016
Chromatographic test strip for determining ligands or receptors Gordon, Julian; McMahon, Michael E.; Ching, Shanfun https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4960691 US Patent Document 4,960,691	patent	October 1990
Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis Pooler, Margaret R.; Hartung, John S. Current Microbiology, Vol. 31, Issue 6 https://doi.org/10.1007/BF00294703	journal	December 1995
Membrane chromatography - an integrative concept in the downstream processing of proteins Thoemmes, J.; Kula, M. -R. Biotechnology Progress, Vol. 11, Issue 4 https://doi.org/10.1021/bp00034a001	journal	July 1995
Identification of Oligonucleotides for the Capture, Detection and Quantitation of West Nile Virus Shyamala, Venkatakrishna https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050032729 US Patent Application 10/729421; 20050032729	patent-application	February 2005
Flow through nucleic acid hybridisation uses thereof and a device thereof Tam, Joseph https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5741647 US Patent Document 5,741,647	patent	April 1998
DNA Microarray Technology Devices, Systems, and Applications Heller, Michael J. Annual Review of Biomedical Engineering, Vol. 4, Issue 1, p. 129-153 https://doi.org/10.1146/annurev.bioeng.4.020702.153438	journal	August 2002
Fluidic Test Cassette Nowakowski, Mark; Wang, Michael; Cary, Robert B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160310948 US Patent Application 15/137983; 20160310948	patent-application	October 2016
gravity-driven apparatus and method for control of microfluidic devices Yao, Nan-Kuang; Wu, Jhy-Wen https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7273590 US Patent Document 7,273,590	patent	September 2007
A novel rapid hybridization technique: paper chromatography hybridization assay (PACHA) Reinhartz, Avraham; Alajem, Sara; Samson, Aline Gene, Vol. 136, Issue 1-2, p. 221-226 https://doi.org/10.1016/0378-1119(93)90468-I	journal	December 1993
Trehalose Is a Potent PCR Enhancer: Lowering of DNA Melting Temperature and Thermal Stabilization of Taq Polymerase by the Disaccharide Trehalose Spiess, Andrej-Nikolai; Mueller, Nadine; Ivell, Richard Clinical Chemistry, Vol. 50, Issue 7 https://doi.org/10.1373/clinchem.2004.031336	journal	July 2004
Influenza vaccines: present and future Palese, Peter; García-Sastre, Adolfo Journal of Clinical Investigation, Vol. 110, Issue 1 https://doi.org/10.1172/JCI0215999	journal	July 2002
Generation and amplification of nucleic acids from ribonucleic acids Missel, Andreas; Löffert, Dirk; Kang, Jie https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6300069 US Patent Document 6,300,069	patent	October 2001
Dependence of the melting temperature of DNA on salt concentration Schildkraut, Carl; Lifson, Shneior Biopolymers, Vol. 3, Issue 2 https://doi.org/10.1002/bip.360030207	journal	April 1965
Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Walker, G. T.; Little, M. C.; Nadeau, J. G. Proceedings of the National Academy of Sciences, Vol. 89, Issue 1, p. 392-396 https://doi.org/10.1073/pnas.89.1.392	journal	January 1992
Chip PCR. II. Investigation of different PCR amplification systems in microbabricated silicon-glass chips Cheng, J. Nucleic Acids Research, Vol. 24, Issue 2 https://doi.org/10.1093/nar/24.2.380	journal	January 1996
Enhancing PCR amplification and sequencing using DNA-binding proteins Rapley, Ralph Molecular Biotechnology, Vol. 2, Issue 3 https://doi.org/10.1007/BF02745882	journal	December 1994
Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare Lim, D. V.; Simpson, J. M.; Kearns, E. A. Clinical Microbiology Reviews, Vol. 18, Issue 4 https://doi.org/10.1128/CMR.18.4.583-607.2005	journal	October 2005
Use of deoxyinosine containing primers to balance primer efficiency in the amplification of nucleic acid molecules Schuster, David M.; Rashtchian, Ayoub https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5578467 US Patent Document 5,578,467	patent	November 1996
Microchromatography and microelectrophoresis on nitrocellulose membranes Přistoupil, T. I. Chromatographic Reviews, Vol. 12, Issue 2, p. 109-125 https://doi.org/10.1016/0009-5907(70)80007-2	journal	May 1970
Nucleic acid amplification utilizing microfluidic devices Enzelberger, Markus M.; Hansen, Carl L.; Liu, JIan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030008308 US Patent Application 10/118466; 20030008308	patent-application	January 2003
Purification and characterization of Thermus thermophilus UvrD Collins, Ruairi; McCarthy, Tommie V. Extremophiles, Vol. 7, Issue 1 https://doi.org/10.1007/s00792-002-0293-4	journal	February 2003
Diagnostic assays including multiplexed lateral flow immunoassays with quantum dots Lambert, James L.; Fisher, Anita M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050250141 US Patent Application 11/094115; 20050250141	patent-application	November 2005
Fate of Pierce's Disease Strains of Xylella fastidiosa in Common Riparian Plants in California Purcell, A. H.; Saunders, S. R. Plant Disease, Vol. 83, Issue 9 https://doi.org/10.1094/PDIS.1999.83.9.825	journal	September 1999
Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system Barbera-Guillem, Emilio; Nelson, M. Bud; Castro, Stephanie https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6261779 US Patent Document 6,261,779	patent	July 2001
Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides Elliott, K.; Hill, D. S.; Lambert, C. Forensic Science International, Vol. 137, Issue 1, p. 28-36 https://doi.org/10.1016/S0379-0738(03)00267-6	journal	October 2003
Rapid screening assay methods and devices Wang, Mark; Li, Limin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5922617 US Patent Document 5,922,617	patent	July 1999
Chip-based isothermal amplification devices and methods Burns, Mark A.; Burke, David T.; Johnson, Brian N. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020172969 US Patent Application 10/113238; 20020172969	patent-application	November 2002
Methods and compositions for amplification of RNA sequences Kurn, Nurith https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7094536 US Patent Document 7,094,536	patent	August 2006
Colonization of the Sharpshooter Vectors, Oncometopia nigricans and Homalodisca coagulata, by Xylem-Limited Bacteria Brlansky, R. H. Phytopathology, Vol. 73, Issue 4 https://doi.org/10.1094/Phyto-73-530	journal	January 1983
The thermodynamic advantage of DNA oligonucleotide 'stacking hybridization' reactions: energetics of a DNA nick Lane, M. Nucleic Acids Research, Vol. 25, Issue 3 https://doi.org/10.1093/nar/25.3.611	journal	February 1997
Fluidic Test Cassette Thomas, Donald J.; Cai, Hong; Cary, Robert B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20180304260 US Patent Application 15/958958; 20180304260	patent-application	October 2018
Controlled flow assay device and method Ohman, Per Ove; Mendel-Hartvig, Ib https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060239859 US Patent Application 10/560214; 20060239859	patent-application	October 2006
Optimal conditions for hybridization with oligonucleotides: A study with myc-oncogene DNA probes Albretsen, Catrine; Haukanes, Bjørn-Ivar; Aasland, Rein Analytical Biochemistry, Vol. 170, Issue 1, p. 193-202 https://doi.org/10.1016/0003-2697(88)90108-X	journal	April 1988
Betaine Improves the PCR Amplification of GC-Rich DNA Sequences Henke, W.; Herdel, K.; Jung, K. Nucleic Acids Research, Vol. 25, Issue 19 https://doi.org/10.1093/nar/25.19.3957	journal	October 1997
Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction. Tsai, Y. L.; Olson, B. H. Applied and Environmental Microbiology, Vol. 58, Issue 7 https://doi.org/10.1128/aem.58.7.2292-2295.1992	journal	January 1992
Biosensor for Dengue Virus Detection: Sensitive, Rapid, and Serotype Specific Baeumner, Antje J.; Schlesinger, Nicole A.; Slutzki, Naomi S. Analytical Chemistry, Vol. 74, Issue 6 https://doi.org/10.1021/ac015675e	journal	March 2002
Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology Zijlmans, H. J. M. A. A.; Bonnet, J.; Burton, J. Analytical Biochemistry, Vol. 267, Issue 1 https://doi.org/10.1006/abio.1998.2965	journal	February 1999
Compositions and Methods for the Specific Detection of Mammalian Muscle Proteins Muldoon, Mark Thomas; Onisk, Dale Vernon; , Michael Craig Brown https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040209309 US Patent Application 10/789433; 20040209309	patent-application	October 2004
Encoded evidence: DNA in forensic analysis Jobling, Mark A.; Gill, Peter Nature Reviews Genetics, Vol. 5, Issue 10 https://doi.org/10.1038/nrg1455	journal	October 2004
Short Shared Nucleotide Sequences Ananiev, Evgueni V. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020058252 US Patent Application 09/730468; 20020058252	patent-application	May 2002
Helicase dependent amplification of nucleic acids Kong, Huimin; Vincent, Myriam; Xu, Yan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040058378 US Patent Application 10/665633; 20040058378	patent-application	March 2004
Nucleic acid sequence-based amplification Compton, J. Nature, Vol. 350, Issue 6313 https://doi.org/10.1038/350091a0	journal	March 1991
Recovery and detection of enterovirus, hepatitis A virus and Norwalk virus in hardshell clams (Mercenaria mercenaria) by RT-PCR methods Suñén, Ester; Sobsey, Mark D. Journal of Virological Methods, Vol. 77, Issue 2, p. 179-187 https://doi.org/10.1016/S0166-0934(98)00148-7	journal	February 1999
One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device Aveyard, Jenny; Mehrabi, Maryam; Cossins, Andrew Chemical Communications, Issue 41 https://doi.org/10.1039/b708859k	journal	January 2007
Use of Single Nucleotide Polymorphisms in the plcR Gene for Specific Identification of Bacillus anthracis Easterday, W. R.; Van Ert, M. N.; Simonson, T. S. Journal of Clinical Microbiology, Vol. 43, Issue 4 https://doi.org/10.1128/JCM.43.4.1995-1997.2005	journal	April 2005
Nucleic Acid Detection System and Method for Detecting Influenza Cai, Hong; Song, Jian https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170233794 US Patent Application 15/498344; 20170233794	patent-application	August 2017
Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils Young, Charles C.; Burghoff, Robert L.; Keim, Lois G. Applied and Environmental Microbiology, Vol. 59, Issue 6 https://doi.org/10.1128/aem.59.6.1972-1974.1993	journal	January 1993
Systems and methods for detection of analytes in biological fluids Kovalenko, Victor https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030003514 US Patent Application 10/138654; 20030003514	patent-application	January 2003
DNA sequencing by primer walking with strings of contiguous hexamers Kieleczawa, J.; Dunn, J.; Studier, F. Science, Vol. 258, Issue 5089 https://doi.org/10.1126/science.1465615	journal	December 1992
Synthesis and hybridization of a series of biotinylated oligonucleotides Cook, Alan F.; Vuocolo, Edmund; Brakel, Christine L. Nucleic Acids Research, Vol. 16, Issue 9 https://doi.org/10.1093/nar/16.9.4077	journal	January 1988
PCR microfluidic devices for DNA amplification Zhang, Chunsun; Xu, Jinliang; Ma, Wenli Biotechnology Advances, Vol. 24, Issue 3 https://doi.org/10.1016/j.biotechadv.2005.10.002	journal	May 2006
A rapid biosensor for viable B. anthracis spores Baeumner, AntjeJ.; Leonard, Barbara; McElwee, John Analytical and Bioanalytical Chemistry, Vol. 380, Issue 1 https://doi.org/10.1007/s00216-004-2726-7	journal	August 2004
Diagnostic applications of microarrays Petrik, J. Transfusion Medicine, Vol. 16, Issue 4 https://doi.org/10.1111/j.1365-3148.2006.00673.x	journal	August 2006
Influenza Webster, Robert; Walker, Elizabeth American Scientist, Vol. 91, Issue 2 https://doi.org/10.1511/2003.2.122	journal	January 2003
Localized microwave heating in microwells for parallel DNA amplification applications Kempitiya, A.; Borca-Tasciuc, Diana A.; Mohamed, Hisham S. Applied Physics Letters, Vol. 94, Issue 6 https://doi.org/10.1063/1.3078273	journal	February 2009
Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format. Kwoh, D. Y.; Davis, G. R.; Whitfield, K. M. Proceedings of the National Academy of Sciences, Vol. 86, Issue 4, p. 1173-1177 https://doi.org/10.1073/pnas.86.4.1173	journal	February 1989
Detection Signal and Capture in Dipstick Assays Lee, Helen; Dineva, Magda Anastassova https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040053256 US Patent Application 10/332133; 20040053256	patent-application	March 2004
Test device and method for its use Deutsch, Marshall E.; Mead, Louis W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4235601 US Patent Document 4,235,601	patent	November 1980
An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA Gill, Peter; Whitaker, Jonathan; Flaxman, Christine Forensic Science International, Vol. 112, Issue 1, p. 17-40 https://doi.org/10.1016/S0379-0738(00)00158-4	journal	July 2000
Association of a Rickettsialike Organism with Pierce's Disease of Grapevines and Alfalfa Dwarf and Heat Therapy of the Disease in Grapevines Goheen, A. C. Phytopathology, Vol. 63, Issue 3 https://doi.org/10.1094/Phyto-63-341	journal	January 1973
Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses Mumford, R. A.; Seal, S. E. Journal of Virological Methods, Vol. 69, Issue 1-2, p. 73-79 https://doi.org/10.1016/S0166-0934(97)00141-9	journal	December 1997
A System for Specific, High-throughput Genotyping by Allele-specific Primer Extension on Microarrays Pastinen, T. Genome Research, Vol. 10, Issue 7 https://doi.org/10.1101/gr.10.7.1031	journal	July 2000
Identifying Influenza Viruses with Resequencing Microarrays Wang, Zheng; Daum, Luke T.; Vora, Gary J. Emerging Infectious Diseases, Vol. 12, Issue 4 https://doi.org/10.3201/eid1204.051441	journal	April 2006
Method for Amplification of DNA Lee, Jar-How; Blair, Lindley https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20010019825 US Patent Application 09/817815; 20010019825	patent-application	September 2001
Real-time PCR in the microbiology laboratory Mackay, I. M. Clinical Microbiology and Infection, Vol. 10, Issue 3 https://doi.org/10.1111/j.1198-743X.2004.00722.x	journal	March 2004
Single nucleotide polymorphism genotyping using locked nucleic acid (LNA™) Mouritzen, Peter; Nielsen, Alex Toftgaard; Pfundheller, Henrik M. Expert Review of Molecular Diagnostics, Vol. 3, Issue 1 https://doi.org/10.1586/14737159.3.1.27	journal	January 2003
Technology platforms for pharmacogenomic diagnostic assays Koch, Walter H. Nature Reviews Drug Discovery, Vol. 3, Issue 9 https://doi.org/10.1038/nrd1496	journal	September 2004
Oscillating amplification reaction for nucleic acids Cai, Hong; Cobb, Nathan Jeremy https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9428781 US Patent Document 9,428,781	patent	August 2016
Amplification and cloning of a long RNA virus genome using immunocapture-long RT-PCR Romero, Alicia; Blanco-Urgoiti, Begoña; Ponz, Fernando Journal of Virological Methods, Vol. 66, Issue 1, p. 159-163 https://doi.org/10.1016/S0166-0934(97)00043-8	journal	June 1997
Microfluidic Cartridge Handique, Kalyan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090130719 US Patent Application 12/239537; 20090130719	patent-application	May 2009
Thermodynamic effects of formamide on DNA stability Blake, R. Nucleic Acids Research, Vol. 24, Issue 11 https://doi.org/10.1093/nar/24.11.2095	journal	June 1996
Method for detection of non-denatured nucleic acid fragments Ebersole, Richard C.; Hendrickson, Edwin R.; Payne, Mark S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6037127 US Patent Document 6,037,127	patent	March 2000
Enrichment of a Target Sequence Makrigiorgos, Gerassimos https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100203532 US Patent Application 12/671295; 20100203532	patent-application	August 2010
Virulence signatures: microarray-based approaches to discovery and analysis Pannucci, James; Cai, Hong; Pardington, Paige E. Biosensors and Bioelectronics, Vol. 20, Issue 4, p. 706-718 https://doi.org/10.1016/j.bios.2004.04.005	journal	November 2004
Oseltamivir Resistance during Treatment of Influenza A (H5N1) Infection de Jong, Menno D.; Thanh, Tran Tan; Khanh, Truong Huu New England Journal of Medicine, Vol. 353, Issue 25 https://doi.org/10.1056/NEJMoa054512	journal	December 2005
A novel, sensitive detection system for high-density microarrays using dendrimer technology Stears, Robin L.; Getts, Robert C.; Gullans, Steven R. Physiological Genomics, Vol. 3, Issue 2 https://doi.org/10.1152/physiolgenomics.2000.3.2.93	journal	August 2000
High surface area substrates for microarrays and methods to make same Agrawal, Anoop; Cronin, John P.; Tonazzi, Juan Carlos L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7195872 US Patent Document 7,195,872	patent	March 2007
Nucleic acid detection system and method for detecting influenza Cai, Hong; Song, Jian https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8980561 US Patent Document 8,980,561	patent	March 2015
Liquid transfer devices Bunce, Roger A.; Thorpe, Gary H. G. H.; Gibbons, John E. C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5354538 US Patent Document 5,354,538	patent	October 1994
Modified Substrates for the Attachment of Biomolecules Pantano, Carlo G.; Metwalli, Ezz; Conzone, Samuel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030054176 US Patent Application 09/947923; 20030054176	patent-application	March 2003
Biosensor and Measurement Method Nadaoka, Masataka; Takahashi, Mie; Tanaka, Hirotaka https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040029177 US Patent Application 10/416272; 20040029177	patent-application	February 2004
Assay devices having detection capabilities within the hook effect region Song, Xuedong; Christopher, Paul https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060246601 US Patent Application 11/119262; 20060246601	patent-application	November 2006
Pierce's Disease Bacterium: Mechanism of Transmission by Leafhopper Vectors Purcell, A. H.; Finlay, A. H.; Mclean, D. L. Science, Vol. 206, Issue 4420 https://doi.org/10.1126/science.206.4420.839	journal	November 1979
A Bead-Based Method for Multiplexed Identification and Quantitation of DNA Sequences Using Flow Cytometry Spiro, A.; Lowe, M.; Brown, D. Applied and Environmental Microbiology, Vol. 66, Issue 10 https://doi.org/10.1128/AEM.66.10.4258-4265.2000	journal	October 2000
Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA Braasch, Dwaine A.; Corey, David R. Chemistry & Biology, Vol. 8, Issue 1, p. 1-7 https://doi.org/10.1016/S1074-5521(00)00058-2	journal	January 2001
Quantitative PCR Method for Diagnosis of Citrus Bacterial Canker Cubero, J.; Graham, J. H.; Gottwald, T. R. Applied and Environmental Microbiology, Vol. 67, Issue 6 https://doi.org/10.1128/AEM.67.6.2849-2852.2001	journal	June 2001
Activity of endonuclease S1 in denaturing solvents: Dimethysulfoxide, dimethylformamide, formamide and formaldehyde Hutton, James R.; Wetmur, James G. Biochemical and Biophysical Research Communications, Vol. 66, Issue 3 https://doi.org/10.1016/0006-291X(75)90731-7	journal	October 1975
Micro-arrayed organization of transcription factor target genes Burgess Jr., Robert M.; Lunyak, Victoria; Noskin, Leonid https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050079492 US Patent Application 10/275845; 20050079492	patent-application	April 2005
Trace DNA: A Review, Discussion of Theory, and Application of the Transfer of Trace Quantities of DNA Through Skin Contact Wickenheiser, R. Journal of Forensic Sciences, Vol. 47, Issue 3 https://doi.org/10.1520/JFS15284J	journal	May 2002
An amplification-free hybridization-based DNA assay to detect Streptococcus pneumoniae utilizing the up-converting phosphor technology Zuiderwijk, Michel; Tanke, Hans J.; Sam Niedbala, R. Clinical Biochemistry, Vol. 36, Issue 5, p. 401-403 https://doi.org/10.1016/S0009-9120(03)00057-2	journal	July 2003
Method of Sample Analysis and Apparatus Therefor Sowerby, Stephen J.; Broom, Murray Frederick; McCaslin, Richard Brian https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080124720 US Patent Application 11/720387; 20080124720	patent-application	May 2008
Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control Cary, Robert B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9944922 US Patent Document 9,944,922	patent	April 2018
Oxygen sensing membranes and methods of making same Berger, Joseph; Collins, Thomas C.; Slovacek, Rudolf E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6190612 US Patent Document 6,190,612	patent	February 2001
Controlled reagent transport in disposable 2D paper networks Fu, Elain; Lutz, Barry; Kauffman, Peter Lab on a Chip, Vol. 10, Issue 7 https://doi.org/10.1039/b919614e	journal	January 2010
One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays Kimura, Naoki Biochemical and Biophysical Research Communications, Vol. 347, Issue 2, p. 477-484 https://doi.org/10.1016/j.bbrc.2006.06.130	journal	August 2006
Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR. Schwab, K. J.; De Leon, R.; Sobsey, M. D. Applied and environmental microbiology, Vol. 62, Issue 6 https://doi.org/10.1128/aem.62.6.2086-2094.1996	journal	January 1996
Multiple Hybrid Immunoassay Campbell, John Lewis Emerson; Frank, Pamela Anne; Hawkes, Meghan Elizabeth https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20140141484 US Patent Application 14/081309; 20140141484	patent-application	May 2014
A simple procedure for enhancing PCR specificity. Weighardt, F.; Biamonti, G.; Riva, S. Genome Research, Vol. 3, Issue 1 https://doi.org/10.1101/gr.3.1.77	journal	August 1993
Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles Masny, A. Nucleic Acids Research, Vol. 31, Issue 18 https://doi.org/10.1093/nar/gng116	journal	September 2003
Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification An, Lixin; Tang, Wen; Ranalli, Tamara A. Journal of Biological Chemistry, Vol. 280, Issue 32, p. 28952-28958 https://doi.org/10.1074/jbc.M503096200	journal	June 2005
Highly Simplified Lateral Flow-Based Nucleic Acid Sample Preparation and Passive Fluid Flow Control Cary, Robert B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160083716 US Patent Application 14/962967; 20160083716	patent-application	March 2016
Amelioration of Heterophile Antibody Immunosensor Interference Campbell, John Lewis Emerson; Omakor, John Emegbero https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100248273 US Patent Application 12/411325; 20100248273	patent-application	September 2010
Nucleic Acid Detection System and Method for Detecting Influenza Cai, Hong; Song, Jian https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20150184255 US Patent Application 14/660773; 20150184255	patent-application	July 2015
DNA helix destabilization by proline and betaine: possible role in the salinity tolerance process Rajendrakumar, Chadalavada S. V.; Suryanarayana, Tangirala; Reddy, Arjula R. FEBS Letters, Vol. 410, Issue 2-3 https://doi.org/10.1016/S0014-5793(97)00588-7	journal	June 1997
Lab-on-a-chip devices for global health: Past studies and future opportunities Chin, Curtis D.; Linder, Vincent; Sia, Samuel K. Lab Chip, Vol. 7, Issue 1 https://doi.org/10.1039/B611455E	journal	January 2007
Displacement assay on a porous membrane Ligler, Frances S.; Kusterbeck, Anne W.; Rabbany, Sina Y. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020028475 US Patent Application 09/907888; 20020028475	patent-application	March 2002
Devices and Methods for Biochip Multiplexing Dong, Hau H.; Blackburn, Gary; Kayyem, Jon F. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020177135 US Patent Application 09/904175; 20020177135	patent-application	November 2002
Accessing Single Nucleotide Polymorphisms in Genomic DNA by Direct Multiplex Polymerase Chain Reaction Amplification on Oligonucleotide Microarrays Huber, Martin; Mündlein, Axel; Dornstauder, Eva Analytical Biochemistry, Vol. 303, Issue 1 https://doi.org/10.1006/abio.2001.5565	journal	April 2002
An Integrated Nanoliter DNA Analysis Device Burns, M. A. Science, Vol. 282, Issue 5388 https://doi.org/10.1126/science.282.5388.484	journal	October 1998
Mobile Rapid Test System for Nucleic Acid Analysis Hillebrand, Timo; Knippschild, Claus; Jaschinsky, Benjamin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20110039261 US Patent Application 12/820569; 20110039261	patent-application	February 2011
Assay device for detecting the presence of an analyte involving sequential delivery of reagents through a liquid circuit Chandler, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6146589 US Patent Document 6,146,589	patent	November 2000
Automated closed-vessel system for in vitro diagnostics based on polymerase chain reaction Findlay, J. B.; Atwood, S. M.; Bergmeyer, L. Clinical Chemistry, Vol. 39, Issue 9 https://doi.org/10.1093/clinchem/39.9.1927	journal	September 1993
Miniaturized Lateral Flow Device for Rapid and Sensitive Detection of Proteins or Nucleic Acids Cary, Robert B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160222442 US Patent Application 15/012804; 20160222442	patent-application	August 2016
Immunoassay of infectious agents Andreotti, Peter E.; Ludwig, George V.; Peruski, Anne Harwood BioTechniques, Vol. 35, Issue 4 https://doi.org/10.2144/03354ss02	journal	October 2003
Biosensors for environmental pollutants and food contaminants Baeumner, Antje J. Analytical and Bioanalytical Chemistry, Vol. 377, Issue 3 https://doi.org/10.1007/s00216-003-2158-9	journal	October 2003
Capture of Single-Stranded DNA Assisted by Oligonucleotide Modules O'Meara, Deirdre; Nilsson, Peter; Nygren, Per-Åke Analytical Biochemistry, Vol. 255, Issue 2 https://doi.org/10.1006/abio.1997.2472	journal	January 1998
Strand displacement amplification—an isothermal, in vitro DNA amplification technique Walker, G. Terrance; Fraiser, Melinda S.; Schram, James L. Nucleic Acids Research, Vol. 20, Issue 7, p. 1691-1696 https://doi.org/10.1093/nar/20.7.1691	journal	January 1992
Method and test strip for determining an analyte Bausback, Jörg https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6335205 US Patent Document 6,335,205	patent	January 2002
Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold dipstick Chanteau, Suzanne; Rahalison, Lila; Ratsitorahina, Mahery International Journal of Medical Microbiology, Vol. 290, Issue 3, p. 279-283 https://doi.org/10.1016/S1438-4221(00)80126-5	journal	July 2000
Advances in Polymerase Chain Reaction on Microfluidic Chips Roper, Michael G.; Easley, Christopher J.; Landers, James P. Analytical Chemistry, Vol. 77, Issue 12 https://doi.org/10.1021/ac050756m	journal	June 2005
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Saiki, R. K.; Gelfand, D. H; Stoffel, S. Science, Vol. 239, Issue 4839, p. 487-491 https://doi.org/10.1126/science.239.4839.487	journal	January 1988
Use of Up-Converting Phosphor Reporters in Lateral-Flow Assays to Detect Specific Nucleic Acid Sequences: A Rapid, Sensitive DNA Test to Identify Human Papillomavirus Type 16 Infection Corstjens, Paul; Zuiderwijk, Michel; Brink, Antoinette Clinical Chemistry, Vol. 47, Issue 10 https://doi.org/10.1093/clinchem/47.10.1885	journal	October 2001
DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence Kaplan, Daniel L.; Steitz, Thomas A. Journal of Biological Chemistry, Vol. 274, Issue 11 https://doi.org/10.1074/jbc.274.11.6889	journal	March 1999
Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses Jacobi, V.; Bachand, G. D.; Hamelin, R. C. Journal of Virological Methods, Vol. 74, Issue 2, p. 167-178 https://doi.org/10.1016/S0166-0934(98)00086-X	journal	October 1998
The total influenza vaccine failure of 1947 revisited: Major intrasubtypic antigenic change can explain failure of vaccine in a post-World War II epidemic Kilbourne, E. D.; Smith, C.; Brett, I. Proceedings of the National Academy of Sciences, Vol. 99, Issue 16, p. 10748-10752 https://doi.org/10.1073/pnas.162366899	journal	July 2002
Detection and Diversity Assessment of Xylella fastidiosa in Field-Collected Plant and Insect Samples by Using 16S rRNA and gyrB Sequences Rodrigues, J. L. M.; Silva-Stenico, M. E.; Gomes, J. E. Applied and Environmental Microbiology, Vol. 69, Issue 7 https://doi.org/10.1128/AEM.69.7.4249-4255.2003	journal	July 2003
Pierce's Disease of Grapevines: Isolation of the Causal Bacterium Davis, M. J.; Purcell, A. H.; Thomson, S. V. Science, Vol. 199, Issue 4324 https://doi.org/10.1126/science.199.4324.75	journal	January 1978
Xylella Fastidiosa: Xylem-Limited Bacterial Pathogen of Plants Hopkins, D. L. Annual Review of Phytopathology, Vol. 27, Issue 1 https://doi.org/10.1146/annurev.py.27.090189.001415	journal	September 1989
Isothermal strand displacement nucleic acid amplification Dattagupta, Nanibhushan; Stull, Paul D.; Spingola, Marc https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6214587 US Patent Document 6,214,587	patent	April 2001
Reaction apparatus employing gravitational flow Andrews, Robert https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5225163 US Patent Document 5,225,163	patent	July 1993
Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. Monteiro, L.; Bonnemaison, D.; Vekris, A. Journal of clinical microbiology, Vol. 35, Issue 4 https://doi.org/10.1128/jcm.35.4.995-998.1997	journal	January 1997
An efficient and economic enhancer mix for PCR Ralser, Markus; Querfurth, Robert; Warnatz, Hans-Jörg Biochemical and Biophysical Research Communications, Vol. 347, Issue 3, p. 747-751 https://doi.org/10.1016/j.bbrc.2006.06.151	journal	September 2006
Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus Zaytseva, NatalyaV.; Montagna, RichardA.; Lee, EunMi Analytical and Bioanalytical Chemistry, Vol. 380, Issue 1 https://doi.org/10.1007/s00216-004-2724-9	journal	July 2004
Nucleic Acid Amplification Method Using Microchip and Microchip, and Nucleic Acid Amplification System Using the Same Iwaki, Yoshihide; Mori, Toshihiro https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080207892 US Patent Application 11/944342; 20080207892	patent-application	August 2008
PCR in a silicon microstructure Wilding, P.; Shoffner, M. A.; Kricka, L. J. Clinical Chemistry, Vol. 40, Issue 9 https://doi.org/10.1093/clinchem/40.9.1815	journal	September 1994
Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method. Hartung, J. S.; Daniel, J. F.; Pruvost, O. P. Applied and Environmental Microbiology, Vol. 59, Issue 4 https://doi.org/10.1128/aem.59.4.1143-1148.1993	journal	January 1993
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Barnes, W. M. Proceedings of the National Academy of Sciences, Vol. 91, Issue 6 https://doi.org/10.1073/pnas.91.6.2216	journal	March 1994
Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip Cheek, Brady J.; Steel, Adam B.; Torres, Matthew P. Analytical Chemistry, Vol. 73, Issue 24 https://doi.org/10.1021/ac0108616	journal	December 2001
Rapid and simple method for purification of nucleic acids. Boom, R.; Sol, C. J.; Salimans, M. M. Journal of Clinical Microbiology, Vol. 28, Issue 3 https://doi.org/10.1128/jcm.28.3.495-503.1990	journal	January 1990
High-performance membrane chromatography: Highly efficient separation method for proteins in ion-exchange, hydrophobic interaction and reversed-phase modes Tennikova, Tatiana B.; Svec, Frantisek Journal of Chromatography A, Vol. 646, Issue 2, p. 279-288 https://doi.org/10.1016/0021-9673(93)83340-X	journal	September 1993
Integrated PCR Reactor for Cell Lysis, Nucleic Acid Isolation and Purification, and Nucleic Acid Amplification Related Applications Mark, Michael G.; Bau, Haim H.; Malamud, Daniel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090186357 US Patent Application 12/331508; 20090186357	patent-application	July 2009
Effects of Amplification Facilitators on Diagnostic PCR in the Presence of Blood, Feces, and Meat Abu Al-Soud, Waleed; Rådström, Peter Journal of Clinical Microbiology, Vol. 38, Issue 12 https://doi.org/10.1128/JCM.38.12.4463-4470.2000	journal	January 2000
Solid supports for nucleic acid hybridization assays Van Ness, Jeffrey; Petrie, Charles R.; Tabone, John C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5514785 US Patent Document 5,514,785	patent	May 1996
Multiplexed, particle-based detection of DNA using flow cytometry with 3DNA dendrimers for signal amplification Lowe, Mary; Spiro, Alex; Zhang, Yu-Zhong Cytometry, Vol. 60A, Issue 2 https://doi.org/10.1002/cyto.a.20021	journal	January 2004
Highly Simplified Lateral Flow-Based Nucleic Acid Sample Preparation and Passive Fluid Flow Control Cary, Robert B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20110117540 US Patent Application 12/940973; 20110117540	patent-application	May 2011
Using a microfluidic device for 1 l DNA microarray hybridization in 500 s Wei, C. -W. Nucleic Acids Research, Vol. 33, Issue 8 https://doi.org/10.1093/nar/gni078	journal	April 2005
Incidence of adamantane resistance among influenza A (H3N2) viruses isolated worldwide from 1994 to 2005: a cause for concern Bright, Rick A.; Medina, Marie-jo; Xu, Xiyan The Lancet, Vol. 366, Issue 9492 https://doi.org/10.1016/S0140-6736(05)67338-2	journal	October 2005
Low temperature cycled PCR protocol for Klenow fragment of DNA polymerase I in the presence of proline Iakobashvili, R. Nucleic Acids Research, Vol. 27, Issue 6 https://doi.org/10.1093/nar/27.6.1566	journal	March 1999
Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography Carter, D. J.; Cary, R. B. Nucleic Acids Research, Vol. 35, Issue 10 https://doi.org/10.1093/nar/gkm269	journal	April 2007
Nucleic Acid Amplification Method Shigemori, Yasushi https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050136443 US Patent Application 10/948193; 20050136443	patent-application	June 2005
Label-Free Potentiometry for Detecting DNA Hybridization Using Peptide Nucleic Acid and DNA Probes Goda, Tatsuro; Singi, Ankit; Maeda, Yasuhiro Sensors, Vol. 13, Issue 2 https://doi.org/10.3390/s130202267	journal	February 2013
Development of a Novel, Rapid Integrated Cryptosporidium parvum Detection Assay Kozwich, D.; Johansen, K. A.; Landau, K. Applied and Environmental Microbiology, Vol. 66, Issue 7 https://doi.org/10.1128/AEM.66.7.2711-2717.2000	journal	July 2000
Betaine can eliminate the base pair composition dependence of DNA melting Rees, William A.; Yager, Thomas D.; Korte, John Biochemistry, Vol. 32, Issue 1 https://doi.org/10.1021/bi00052a019	journal	January 1993
Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA Leone, G.; van Schijndel, H. B.; van Genien, B. Journal of Virological Methods, Vol. 66, Issue 1, p. 19-27 https://doi.org/10.1016/S0166-0934(97)02203-9	journal	June 1997
Chromatographic performance of a thin microporous bed of nitrocellulose Lönnberg, Maria; Carlsson, Jan Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 763, Issue 1-2, p. 107-120 https://doi.org/10.1016/S0378-4347(01)00376-0	journal	November 2001
Sample-efficient lateral flow immunoassay Kaylor, Rosann Marie; Wei, Ning; Chidebelu-Eze, Chibueze https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060127886 US Patent Application 11/012759; 20060127886	patent-application	June 2006
Process for amplifying nucleic acid sequences Mullis, Kary B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683202 US Patent Document 4,683,202	patent	July 1987
Process for amplifying, detecting, and/or cloning nucleic acid sequences Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4800159 US Patent Document 4,800,159	patent	January 1989
Design Strategies and Performance of Custom DNA Sequencing Primers Buck, G. A.; Fox, J. W.; Gunthorpe, M. BioTechniques, Vol. 27, Issue 3 https://doi.org/10.2144/99273rr01	journal	September 1999
The ligase chain reaction in a PCR world. Barany, F. Genome Research, Vol. 1, Issue 1 https://doi.org/10.1101/gr.1.1.5	journal	August 1991
Biosensor for the specific detection of a single viable B. anthracis spore Hartley, Harriet A.; Baeumner, Antje J. Analytical and Bioanalytical Chemistry, Vol. 376, Issue 3 https://doi.org/10.1007/s00216-003-1939-5	journal	May 2003
Biochemical Characterization of the DNA Helicase Activity of the Escherichia coli RecQ Helicase Harmon, Frank G.; Kowalczykowski, Stephen C. Journal of Biological Chemistry, Vol. 276, Issue 1 https://doi.org/10.1074/jbc.M006555200	journal	October 2000
Specific detection of Bacillus anthracis using aTaqMan® mismatch amplification mutation assay Easterday, William R.; Van Ert, Matthew N.; Zanecki, Shaylan BioTechniques, Vol. 38, Issue 5 https://doi.org/10.2144/05385ST03	journal	May 2005
Potential Impact of Antiviral Drug Use during Influenza Pandemic Gani, Raymond; Hughes, Helen; Fleming, Douglas Emerging Infectious Diseases, Vol. 11, Issue 9 https://doi.org/10.3201/eid1209.041344	journal	September 2005
Isolation, Culture, and Pathogenicity of the Bacterium Causing Phony Disease of Peach Wells, John M. Phytopathology, Vol. 73, Issue 6 https://doi.org/10.1094/Phyto-73-859	journal	January 1983
Method for extending the dynamic detection range of assay devices Song, Xuedong https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050112780 US Patent Application 10/719976; 20050112780	patent-application	May 2005
Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns Berthelet, Marc; Whyte, Lyle G.; Greer, Charles W. FEMS Microbiology Letters, Vol. 138, Issue 1, p. 17-22 https://doi.org/10.1111/j.1574-6968.1996.tb08128.x	journal	April 1996
Droplet-based micro oscillating-flow PCR chip Wang, Wei; Li, Zhi-Xin; Luo, Rong Journal of Micromechanics and Microengineering, Vol. 15, Issue 8 https://doi.org/10.1088/0960-1317/15/8/001	journal	May 2005
Specific Bonding Analysis Method and Specific Bonding Analysis Device Using It Kenjyou, Noriko; Kamei, Akihito; Kawamura, Tatsurou https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030100128 US Patent Application 10/257009; 20030100128	patent-application	May 2003
Helicase-dependent amplification of nucleic acids Kong, Huimin; Vincent, Myriam; Xu, Yan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060154286 US Patent Application 11/327175; 20060154286	patent-application	July 2006
A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers Ciapina, L. P.; Carareto Alves, L. M.; Lemos, E. G. M. Journal of Applied Microbiology, Vol. 96, Issue 3 https://doi.org/10.1111/j.1365-2672.2004.02176.x	journal	March 2004
Developing rapid, point-of-care, multiplex detection for use in lateral flow devices Rao, R. S.; Albala, J. S.; Lane, S. L. Optics East 2005, SPIE Proceedings https://doi.org/10.1117/12.630157	conference	November 2005
Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection Liisa, Marja; Wada, H. Garrett; Dubrow, Robert S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020179445 US Patent Application 10/142263; 20020179445	patent-application	December 2002
Systems and Methods for Testing Using Microfluidic Chips Chen, Zongyuan G.; Wang, Jing; Mauk, Michael https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080280285 US Patent Application 11/937,975; 2008/0280285 Al	patent-application	November 2008
Sequence-dependent gene sorting techniques Ulanovsky, Levy; Mugasimangalam, Raja C.; Einat, Paz https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6468749 US Patent Document 6,468,749	patent	October 2002
DNA Detection Using Recombination Proteins Piepenburg, Olaf; Williams, Colin H.; Stemple, Derek L. PLoS Biology, Vol. 4, Issue 7 https://doi.org/10.1371/journal.pbio.0040204	journal	June 2006
Apparatus and method for calibration of a microarray scanning system Noblett, David Allan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6471916 US Patent Document 6,471,916	patent	October 2002
Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection Goldmeyer, James; Kong, Huimin; Tang, Wen The Journal of Molecular Diagnostics, Vol. 9, Issue 5 https://doi.org/10.2353/jmoldx.2007.070012	journal	November 2007
Enzymatic synthesis of repeat regions of oligonucleotides Hyman, Edward https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5516664 US Patent Document 5,516,664	patent	May 1996
Cooperative Oligonucleotides Mediating Direct Capture of Hepatitis C Virus RNA from Serum O’Meara, Deirdre; Yun, Zhibing; Sönnerborg, Anders Journal of Clinical Microbiology, Vol. 36, Issue 9 https://doi.org/10.1128/JCM.36.9.2454-2459.1998	journal	January 1998
Microfluidic Device for Rapid (<15 min) Automated Microarray Hybridization Peytavi, R. Clinical Chemistry, Vol. 51, Issue 10 https://doi.org/10.1373/clinchem.2005.052845	journal	October 2005
Self-Contained, Fully Integrated Biochip for Sample Preparation, Polymerase Chain Reaction Amplification, and DNA Microarray Detection Liu, Robin Hui; Yang, Jianing; Lenigk, Ralf Analytical Chemistry, Vol. 76, Issue 7 https://doi.org/10.1021/ac0353029	journal	April 2004
Methods and Compositions for Polynucleotide Amplification Chakrabarti, Raj; Schutt, Clarence https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050042627 US Patent Application 10/792404; 20050042627	patent-application	February 2005
A coupled complex of T4 DNA replication helicase (gp41) and polymerase (gp43) can perform rapid and processive DNA strand-displacement synthesis Dong, F.; Weitzel, S. E.; von Hippel, P. H. Proceedings of the National Academy of Sciences, Vol. 93, Issue 25 https://doi.org/10.1073/pnas.93.25.14456	journal	December 1996
Integrated Device for Nucleic Acid Detection and Identification DeJohn, Marc; Cary, Robert B.; Cobb, Nathan J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20140045191 US Patent Application 14/113146; 20140045191	patent-application	February 2014
Use of photopolymerization for amplification and detection of a molecular recognition event Rowlen, Kathy L.; Birks, John W.; Bowman, Christopher https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060286570 US Patent Application 11/372485; 20060286570	patent-application	December 2006
Transmission-based optical detection systems Cohen, David Samuel; Song, Xuedong; Knotts, Michael https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050243321 US Patent Application 11/022287; 20050243321	patent-application	November 2005
Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia Ilyushina, Natalia A.; Govorkova, Elena A.; Webster, Robert G. Virology, Vol. 341, Issue 1, p. 102-106 https://doi.org/10.1016/j.virol.2005.07.003	journal	October 2005
Molecular Crowding Regulates the Structural Switch of the DNA G-Quadruplex ^† Miyoshi, Daisuke; Nakao, Akihiro; Sugimoto, Naoki Biochemistry, Vol. 41, Issue 50 https://doi.org/10.1021/bi020412f	journal	December 2002
Detection of *Xylella fastidiosa* in potential insect vectors by immunomagnetic separation and nested polymerase chain reaction Pooler, M. R.; Myung, I. S.; Bentz, J. Letters in Applied Microbiology, Vol. 25, Issue 2 https://doi.org/10.1046/j.1472-765X.1997.00188.x	journal	August 1997
Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences Musso, Marco; Bocciardi, Renata; Parodi, Sara The Journal of Molecular Diagnostics, Vol. 8, Issue 5 https://doi.org/10.2353/jmoldx.2006.060058	journal	November 2006
Virus detection method and materials Wang, Chia-Gee https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4663277 US Patent Document 4,663,277	patent	May 1987
Chromatographic Material Brandt, Marcus K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/3667607 US Patent Document 3667607	patent	June 1972
Identification of the Heme Compound Copurified with Deoxyribonucleic Acid (DNA) from Bloodstains, a Major Inhibitor of Polymerase Chain Reaction (PCR) Amplification Akane, Atsushi; Matsubara, Kazuo; Nakamura, Hiroaki Journal of Forensic Sciences, Vol. 39, Issue 2 https://doi.org/10.1520/JFS13607J	journal	March 1994
RNA replication: required intermediates and the dissociation of template, product, and Q.beta. replicase Dobkin, Carl; Mills, Donald R.; Kramer, Fred Russell Biochemistry, Vol. 18, Issue 10 https://doi.org/10.1021/bi00577a030	journal	May 1979
Evaluation of NucliSens easyMAG for Automated Nucleic Acid Extraction from Various Clinical Specimens Loens, K.; Bergs, K.; Ursi, D. Journal of Clinical Microbiology, Vol. 45, Issue 2 https://doi.org/10.1128/JCM.00894-06	journal	December 2006
Detection of foodborne pathogens using DNA probes and a dipstick format Groody, E. Patrick Molecular Biotechnology, Vol. 6, Issue 3 https://doi.org/10.1007/BF02761710	journal	December 1996
Rapid purification of hepatitis B virus DNA from serum. Boom, R.; Sol, C. J.; Heijtink, R. Journal of Clinical Microbiology, Vol. 29, Issue 9 https://doi.org/10.1128/jcm.29.9.1804-1811.1991	journal	January 1991
Immobilization of polynucleotides on magnetic particles. Factors influencing hybridization efficiency Day, P. J.; Flora, P. S.; Fox, J. E. Biochemical Journal, Vol. 278, Issue 3 https://doi.org/10.1042/bj2780735	journal	September 1991
Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA Koonjul, P. K.; Brandt, W. F.; Lindsey, G. G. Nucleic Acids Research, Vol. 27, Issue 3 https://doi.org/10.1093/nar/27.3.915	journal	February 1999
Inhibition and facilitation of nucleic acid amplification. Wilson, I. G. Applied and environmental microbiology, Vol. 63, Issue 10 https://doi.org/10.1128/aem.63.10.3741-3751.1997	journal	January 1997
Oscillating Amplification Reaction for Nucleic Acids Cai, Hong; Cobb, Nathan J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160362725 US Patent Application 15/247728; 20160362725	patent-application	December 2016
Multiplex PCR: Critical Parameters and Step-by-Step Protocol Henegariu, O.; Heerema, N. A.; Dlouhy, S. R. BioTechniques, Vol. 23, Issue 3 https://doi.org/10.2144/97233rr01	journal	September 1997
Capture and detection of target nucleic acid in dipstick assays Lee, Helen; Dineva, Magda Anastassova; Hazlewood, Shaun Christopher https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7186508 US Patent Document 7,186,508	patent	March 2007
Nano-mechanic microsensors and methods for detecting target analytes Warthoe, Peter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060024813 US Patent Application 11/134821; 20060024813	patent-application	February 2006
Lateral Flow Diagnostic Devices with Instrument Controlled Fluidics Lauks, Imants; Pierce, Raymond J.; Wojtyk, James https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050047972 US Patent Application 10/649683; 20050047972	patent-application	March 2005
Microfluidic devices comprising biochannels Blackburn, Gary https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030190608 US Patent Application 09/861171; 20030190608	patent-application	October 2003
Point-of-care diagnostics: will the hurdles be overcome this time? Huckle, David Expert Review of Medical Devices, Vol. 3, Issue 4 https://doi.org/10.1586/17434440.3.4.421	journal	July 2006
Lateral flow assay device and method Cardy, Donald Leonard Nicholas; Allen, Gerard John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060160078 US Patent Application 10/521111; 20060160078	patent-application	July 2006
Nanoparticles Having Oligonucleotides Attached Thereto and Uses Therefor Mirkin, Chad A.; Letsinger, Robert L.; Mucic, Robert C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020127574 US Patent Application 09/973788; 20020127574	patent-application	September 2002
Methods and Devices for Microfluidic Point-of-Care Immunoassays Battrell, C. Frederick; Gerdes, John; Mordue, Stephen https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090181411 US Patent Application 12/341637; 20090181411	patent-application	July 2009
Rapid method for visual identification of specific DNA sequences based on DNA-tagged liposomes Rule, G. S.; Montagna, R. A.; Durst, R. A. Clinical Chemistry, Vol. 42, Issue 8 https://doi.org/10.1093/clinchem/42.8.1206	journal	August 1996
Characteristics and Applications of Nucleic Acid Sequence-Based Amplification (NASBA) Deiman, Birgit; van Aarle, Pierre; Sillekens, Peter Molecular Biotechnology, Vol. 20, Issue 2 https://doi.org/10.1385/MB:20:2:163	journal	January 2002
DNA analysis with multiplex microarray-enhanced PCR Pemov, A. Nucleic Acids Research, Vol. 33, Issue 2 https://doi.org/10.1093/nar/gnh184	journal	January 2005
Influenza Nicholson, Karl G.; Wood, John M.; Zambon, Maria The Lancet, Vol. 362, Issue 9397 https://doi.org/10.1016/S0140-6736(03)14854-4	journal	November 2003
Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity Chatterjee, Deb K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5716819 US Patent Document 5,716,819	patent	February 1998
Culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain ofXylella fastidiosa Chang, Chung J.; Garnier, Monique; Zreik, Leyla Current Microbiology, Vol. 27, Issue 3 https://doi.org/10.1007/BF01576010	journal	September 1993
Method of adjusting the working range of a multi-analyte assay Dowd, Roger; Donahue, Jeffrey G. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060177873 US Patent Application 11/221198; 20060177873	patent-application	August 2006
Method and apparatus for washing magnetically responsive particles Hoet, Rene; Hoogenboom, Henricus Renerus Jacobus Mattheus; Pieters, Henk https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030170686 US Patent Application 10/313822; 20030170686	patent-application	September 2003
Process for amplifying, detecting, and/or-cloning nucleic acid sequences Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683195 US Patent Document 4,683,195	patent	July 1987
Collection device for single step assay of oral fluids Mink, Ronald W.; Goldstein, Andrew S.; Bohannon, Robert C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020192839 US Patent Application 10/076596; 20020192839	patent-application	December 2002
Electrically opened micro fluid valve Broyer, Patrick; Colin, Bruno; Roller, Denis https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7159618 US Patent Document 7,159,618	patent	January 2007
PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings Yang, Samuel; Rothman, Richard E. The Lancet Infectious Diseases, Vol. 4, Issue 6, p. 337-348 https://doi.org/10.1016/S1473-3099(04)01044-8	journal	June 2004
NASBA^TM isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection Kievits, Tim; van Gemen, Bob; van Strijp, Dianne Journal of Virological Methods, Vol. 35, Issue 3, p. 273-286 https://doi.org/10.1016/0166-0934(91)90069-C	journal	December 1991
A ligase-mediated gene detection technique Landegren, U.; Kaiser, R.; Sanders, J. Science, Vol. 241, Issue 4869 https://doi.org/10.1126/science.3413476	journal	August 1988
Single Use Microfluidic Valve Allain, Marjolaine; Basrour, Skandar; Berthier, Jean https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100276005 US Patent Application 12/766231; 20100276005	patent-application	November 2010
Nucleic acid analysis techniques Lockhart, David J.; Chee, Mark; Gunderson, Kevin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030064364 US Patent Application 09/880727; 20030064364	patent-application	April 2003
Barriers for Facilitating Biological Reactions Kelso, David M.; Sur, Kunal; Parpia, Zaheer https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090246782 US Patent Application 12/395020; 20090246782	patent-application	October 2009
Colorimetric detection of immobilised PCR products generated on a solid support Lockley, A. Nucleic Acids Research, Vol. 25, Issue 6 https://doi.org/10.1093/nar/25.6.1313	journal	March 1997
Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content. Baskaran, N.; Kandpal, R. P.; Bhargava, A. K. Genome Research, Vol. 6, Issue 7 https://doi.org/10.1101/gr.6.7.633	journal	July 1996

Similar Records

Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography

Journal Article · Sun Apr 22 00:00:00 EDT 2007 · Nucleic Acids Research · OSTI ID:1600229

Carter, D. J.; Cary, R. B.

Nucleic acid detection system and method for detecting influenza

Patent · Tue Mar 17 00:00:00 EDT 2015 · OSTI ID:1600229

Cai, Hong; Song, Jian

Nucleic acid sequence detection using multiplexed oligonucleotide PCR

Patent · Tue Dec 26 00:00:00 EST 2006 · OSTI ID:1600229

Nolan, John P; White, P Scott

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Title: Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids

Citation Formats

References (294)

Similar Records

Related Subjects