Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

Cary, Robert E.

Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

Patent · 07 December 2015

OSTI ID:1228041

Cary, Robert E.

Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC52-06NA25396

Assignee:: Los Alamos National Security, LLC

Patent Number(s):: 9,207,236

Application Number:: 12/940,973

OSTI ID:: 1228041

Country of Publication:: United States

Language:: English

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A rapid biosensor for viable B. anthracis spores Baeumner, AntjeJ.; Leonard, Barbara; McElwee, John Analytical and Bioanalytical Chemistry, Vol. 380, Issue 1 https://doi.org/10.1007/s00216-004-2726-7	journal	August 2004
Development of a Novel, Rapid Integrated Cryptosporidium parvum Detection Assay Kozwich, D.; Johansen, K. A.; Landau, K. Applied and Environmental Microbiology, Vol. 66, Issue 7 https://doi.org/10.1128/AEM.66.7.2711-2717.2000	journal	July 2000
The thermodynamic advantage of DNA oligonucleotide 'stacking hybridization' reactions: energetics of a DNA nick Lane, M. Nucleic Acids Research, Vol. 25, Issue 3 https://doi.org/10.1093/nar/25.3.611	journal	February 1997
Characteristics and Applications of Nucleic Acid Sequence-Based Amplification (NASBA) Deiman, Birgit; van Aarle, Pierre; Sillekens, Peter Molecular Biotechnology, Vol. 20, Issue 2 https://doi.org/10.1385/MB:20:2:163	journal	January 2002
Amplification and cloning of a long RNA virus genome using immunocapture-long RT-PCR Romero, Alicia; Blanco-Urgoiti, Begoña; Ponz, Fernando Journal of Virological Methods, Vol. 66, Issue 1, p. 159-163 https://doi.org/10.1016/S0166-0934(97)00043-8	journal	June 1997
Point-of-care diagnostics: will the hurdles be overcome this time? Huckle, David Expert Review of Medical Devices, Vol. 3, Issue 4 https://doi.org/10.1586/17434440.3.4.421	journal	July 2006
DNA Detection Using Recombination Proteins Piepenburg, Olaf; Williams, Colin H.; Stemple, Derek L. PLoS Biology, Vol. 4, Issue 7 https://doi.org/10.1371/journal.pbio.0040204	journal	June 2006
Influenza Nicholson, Karl G.; Wood, John M.; Zambon, Maria The Lancet, Vol. 362, Issue 9397 https://doi.org/10.1016/S0140-6736(03)14854-4	journal	November 2003
Chromatographic performance of a thin microporous bed of nitrocellulose Lönnberg, Maria; Carlsson, Jan Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 763, Issue 1-2, p. 107-120 https://doi.org/10.1016/S0378-4347(01)00376-0	journal	November 2001
Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture Jacobsen, N. Nucleic Acids Research, Vol. 32, Issue 7 https://doi.org/10.1093/nar/gnh056	journal	April 2004
Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum Fukuta, Shiro; Ohishi, Kazushi; Yoshida, Keiko Journal of Virological Methods, Vol. 121, Issue 1, p. 49-55 https://doi.org/10.1016/j.jviromet.2004.05.016	journal	October 2004

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