skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nucleic acid detection system and method for detecting influenza

Abstract

The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.

Inventors:
;
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1172753
Patent Number(s):
8,980,561
Application Number:
11/894,908
Assignee:
Los Alamos National Security, LLC. (Los Alamos, NM) LANL
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Aug 22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Cai, Hong, and Song, Jian. Nucleic acid detection system and method for detecting influenza. United States: N. p., 2015. Web.
Cai, Hong, & Song, Jian. Nucleic acid detection system and method for detecting influenza. United States.
Cai, Hong, and Song, Jian. Tue . "Nucleic acid detection system and method for detecting influenza". United States. https://www.osti.gov/servlets/purl/1172753.
@article{osti_1172753,
title = {Nucleic acid detection system and method for detecting influenza},
author = {Cai, Hong and Song, Jian},
abstractNote = {The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 17 00:00:00 EDT 2015},
month = {Tue Mar 17 00:00:00 EDT 2015}
}

Patent:

Save / Share:

Works referenced in this record:

Recovery and detection of enterovirus, hepatitis A virus and Norwalk virus in hardshell clams (Mercenaria mercenaria) by RT-PCR methods
journal, February 1999


Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses
journal, December 1997


Optimal conditions for hybridization with oligonucleotides: A study with myc-oncogene DNA probes
journal, April 1988

  • Albretsen, Catrine; Haukanes, Bjørn-Ivar; Aasland, Rein
  • Analytical Biochemistry, Vol. 170, Issue 1, p. 193-202
  • DOI: 10.1016/0003-2697(88)90108-X

Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification
journal, June 2005

  • An, Lixin; Tang, Wen; Ranalli, Tamara A.
  • Journal of Biological Chemistry, Vol. 280, Issue 32, p. 28952-28958
  • DOI: 10.1074/jbc.M503096200

Current Protocols in Molecular Biology
book, May 2001


Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns
journal, April 1996


Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA
journal, January 2001


Helicase structure and mechanism
journal, February 2002


Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold dipstick
journal, July 2000

  • Chanteau, Suzanne; Rahalison, Lila; Ratsitorahina, Mahery
  • International Journal of Medical Microbiology, Vol. 290, Issue 3, p. 279-283
  • DOI: 10.1016/S1438-4221(00)80126-5

Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides
journal, October 2003


Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum
journal, October 2004


An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA
journal, July 2000

  • Gill, Peter; Whitaker, Jonathan; Flaxman, Christine
  • Forensic Science International, Vol. 112, Issue 1, p. 17-40
  • DOI: 10.1016/S0379-0738(00)00158-4

Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.
journal, March 1990

  • Guatelli, J. C.; Whitfield, K. M.; Kwoh, D. Y.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 5, p. 1874-1878
  • DOI: 10.1073/pnas.87.5.1874

DNA Microarray Technology Devices, Systems, and Applications
journal, August 2002


Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia
journal, October 2005

  • Ilyushina, Natalia A.; Govorkova, Elena A.; Webster, Robert G.
  • Virology, Vol. 341, Issue 1, p. 102-106
  • DOI: 10.1016/j.virol.2005.07.003

Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses
journal, October 1998


NASBATM isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection
journal, December 1991

  • Kievits, Tim; van Gemen, Bob; van Strijp, Dianne
  • Journal of Virological Methods, Vol. 35, Issue 3, p. 273-286
  • DOI: 10.1016/0166-0934(91)90069-C

The total influenza vaccine failure of 1947 revisited: Major intrasubtypic antigenic change can explain failure of vaccine in a post-World War II epidemic
journal, July 2002

  • Kilbourne, E. D.; Smith, C.; Brett, I.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 16, p. 10748-10752
  • DOI: 10.1073/pnas.162366899

One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays
journal, August 2006

  • Kimura, Naoki
  • Biochemical and Biophysical Research Communications, Vol. 347, Issue 2, p. 477-484
  • DOI: 10.1016/j.bbrc.2006.06.130

Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989

  • Kwoh, D. Y.; Davis, G. R.; Whitfield, K. M.
  • Proceedings of the National Academy of Sciences, Vol. 86, Issue 4, p. 1173-1177
  • DOI: 10.1073/pnas.86.4.1173

Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA
journal, June 1997


Chromatographic performance of a thin microporous bed of nitrocellulose
journal, November 2001

  • Lönnberg, Maria; Carlsson, Jan
  • Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 763, Issue 1-2, p. 107-120
  • DOI: 10.1016/S0378-4347(01)00376-0

Virulence signatures: microarray-based approaches to discovery and analysis
journal, November 2004

  • Pannucci, James; Cai, Hong; Pardington, Paige E.
  • Biosensors and Bioelectronics, Vol. 20, Issue 4, p. 706-718
  • DOI: 10.1016/j.bios.2004.04.005

Microchromatography and microelectrophoresis on nitrocellulose membranes
journal, May 1970


A novel rapid hybridization technique: paper chromatography hybridization assay (PACHA)
journal, December 1993


Amplification and cloning of a long RNA virus genome using immunocapture-long RT-PCR
journal, June 1997

  • Romero, Alicia; Blanco-Urgoiti, Begoña; Ponz, Fernando
  • Journal of Virological Methods, Vol. 66, Issue 1, p. 159-163
  • DOI: 10.1016/S0166-0934(97)00043-8

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
journal, January 1988


An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography
journal, January 2000


High-performance membrane chromatography: Highly efficient separation method for proteins in ion-exchange, hydrophobic interaction and reversed-phase modes
journal, September 1993


Isothermal reactions for the amplification of oligonucleotides
journal, April 2003

  • Van Ness, J.; Van Ness, L. K.; Galas, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 8, p. 4504-4509
  • DOI: 10.1073/pnas.0730811100

Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992

  • Walker, G. Terrance; Fraiser, Melinda S.; Schram, James L.
  • Nucleic Acids Research, Vol. 20, Issue 7, p. 1691-1696
  • DOI: 10.1093/nar/20.7.1691

A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection
journal, September 1992


PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings
journal, June 2004


An amplification-free hybridization-based DNA assay to detect Streptococcus pneumoniae utilizing the up-converting phosphor technology
journal, July 2003