Multi-Wavelength Based Optical Density Sensor for Autonomous Monitoring of Microalgae

Jia, Fei; Kacira, Murat; Ogden, Kimberly

doi:10.3390/s150922234

Multi-Wavelength Based Optical Density Sensor for Autonomous Monitoring of Microalgae

Journal Article · Wed Sep 02 00:00:00 EDT 2015 · Sensors

DOI:https://doi.org/10.3390/s150922234· OSTI ID:1592715

Jia, Fei ^[1]; Kacira, Murat ^[2]; Ogden, Kimberly ^[2]

Univ. of Arizona, Tucson, AZ (United States); University of Arizona
Univ. of Arizona, Tucson, AZ (United States)

A multi-wavelength based optical density sensor unit was designed, developed, and evaluated to monitor microalgae growth in real time. The system consisted of five main components including (1) laser diode modules as light sources (2) photodiodes as detectors, (3) driver circuit, (4) flow cell and (5) sensor housing temperature controller. The sensor unit was designed to be integrated into any microalgae culture system for both real time and non-real time optical density measurements and algae growth monitoring applications. It was shown that the sensor unit was capable of monitoring the dynamics and physiological changes of the microalgae culture in real-time. Algae biomass concentration was accurately estimated with optical density measurements at 650, 685 and 780 nm wavelengths used by the sensor unit. The sensor unit was able to monitor cell concentration as high as 1.05 g L^-1 (1.51x10⁸ cells mL^-1) during the culture growth without any sample preparation for the measurements. Since high cell concentrations do not need to be diluted using the sensor unit, the system has the potential to be used in industrial microalgae cultivation systems for real time monitoring and control applications that can lead to improved resource use efficiency.

View Accepted Manuscript (DOE)

Research Organization:: Arizona State Univ., Tucson, AZ (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)

Grant/Contract Number:: EE0006269

OSTI ID:: 1592715

Journal Information:: Sensors, Journal Name: Sensors Journal Issue: 9 Vol. 15; ISSN SENSC9; ISSN 1424-8220

Publisher:: MDPI AGCopyright Statement

Country of Publication:: United States

Language:: English

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09 BIOMASS FUELS
42 ENGINEERING
microalgae
multi-wavelength
optical density
real-time monitoring and control

Multi-Wavelength Based Optical Density Sensor for Autonomous Monitoring of Microalgae

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References (25)

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