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Title: Devices, systems, and methods for detecting nucleic acids using sedimentation

Abstract

Embodiments of the present invention are directed toward devices, systems, and method for conducting nucleic acid purification and quantification using sedimentation. In one example, a method includes generating complexes which bind to a plurality of beads in a fluid sample, individual ones of the complexes comprising a nucleic acid molecule such as DNA or RNA and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

Inventors:
; ;
Publication Date:
Research Org.:
National Technology & Engineering Solutions of Sandia, LLC, Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1568733
Patent Number(s):
10,384,202
Application Number:
15/704,860
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
DOE Contract Number:  
NA0003525
Resource Type:
Patent
Resource Relation:
Patent File Date: 09/14/2017
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory J. Devices, systems, and methods for detecting nucleic acids using sedimentation. United States: N. p., 2019. Web.
Koh, Chung-Yan, Schaff, Ulrich Y., & Sommer, Gregory J. Devices, systems, and methods for detecting nucleic acids using sedimentation. United States.
Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory J. Tue . "Devices, systems, and methods for detecting nucleic acids using sedimentation". United States. https://www.osti.gov/servlets/purl/1568733.
@article{osti_1568733,
title = {Devices, systems, and methods for detecting nucleic acids using sedimentation},
author = {Koh, Chung-Yan and Schaff, Ulrich Y. and Sommer, Gregory J.},
abstractNote = {Embodiments of the present invention are directed toward devices, systems, and method for conducting nucleic acid purification and quantification using sedimentation. In one example, a method includes generating complexes which bind to a plurality of beads in a fluid sample, individual ones of the complexes comprising a nucleic acid molecule such as DNA or RNA and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {8}
}

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