skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Devices, systems, and methods for detecting nucleic acids using sedimentation

Patent ·
OSTI ID:1568733
; ;

Embodiments of the present invention are directed toward devices, systems, and method for conducting nucleic acid purification and quantification using sedimentation. In one example, a method includes generating complexes which bind to a plurality of beads in a fluid sample, individual ones of the complexes comprising a nucleic acid molecule such as DNA or RNA and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

Research Organization:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
DOE Contract Number:
NA0003525
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
Patent Number(s):
10,384,202
Application Number:
15/704,860
OSTI ID:
1568733
Resource Relation:
Patent File Date: 09/14/2017
Country of Publication:
United States
Language:
English

References (64)

Microparticle-based methods and systems and applications thereof patent-application December 2005
Integrated planar microfluidic bioanalytical systems patent-application August 2006
Method for Separating Cells patent-application May 2008
Diagnostic tests and kits for Clostridium difficile patent January 2003
Systems, Devices, and Methods For Agglutination Assays Using Sedimentation patent-application March 2016
Method and apparatus for measuring blood constituent counts patent July 1987
DNA purification and isolation using magnetic particles patent January 1998
Method and Apparatus For Precise Transfer and Manipulation Of Fluids By Centrifugal and Or Capillary Forces patent-application January 2009
Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto patent January 2007
Class characterization of circulating cancer cells isolated from body fluids and methods of use patent-application July 2002
Diffusion-based system and method for detecting and monitoring activity of biologic and chemical species patent-application October 2003
Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay patent June 1997
Centrifugal force based microfluidic device, microfluidic system including the same, and method of determining home position of the microfluidic device patent July 2010
Toxin Activity Assays, Devices, Methods And Systems Therefor patent-application June 2016
System and method for detecting components of a mixture including a valving scheme for competition assays patent July 2017
Multichannel control in microfluidics patent-application October 2002
Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation patent February 2015
Multistation, Single Channel Analytical Photometer and Method of Use patent January 1971
Analytical methods and compositions patent-application November 2002
Centripetally-motivated microfluidics system for performing in vitro hybridization and amplification of nucleic acids patent February 2008
Multi-parameter assays including analysis discs and methods relating thereto patent-application October 2002
Microfluidic devices and methods patent-application January 2003
Microfluidic Devices, Systems, And Methods For Quantifying Particles Using Centrifugal Force patent-application December 2015
Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system patent November 2001
Diagnostic Analyzers With Pretreatment Carousels and Related Methods patent-application September 2014
System and method for detecting components of a mixture including tooth elements for alignment patent November 2016
Separation of solid and liquid components of mixtures patent November 1978
Devices, systems, and methods for conducting sandwich assays using sedimentation patent February 2015
Microfluidic devices, systems, and methods for quantifying particles using centrifugal force patent November 2015
Apparatus and method for measuring white blood cell and platelet concentrations in blood patent May 1979
Microfluidic particle-analysis systems patent-application April 2004
Loading Disk for Photometric Analyzer of Rotary Cuvette Type patent July 1973
Multi-parameter assays including analysis discs and methods relating thereto patent April 2006
Separation Device patent-application May 2010
Centrifugation Apparatus For Containers Of Biological Material patent-application February 2011
Apparatus for precise transfer and manipulation of fluids by centrifugal and or capillary forces patent December 2012
Method for separating the cellular components of blood samples patent July 1989
Reagents for labeling biomolecules having aldehyde or ketone Moieties patent-application September 2002
Plastic microfluidics enabling two-dimensional protein separations in proteome analysis patent-application November 2002
Density-based cell detection system patent March 2010
Protecting agent patent-application August 2005
Apparatus and method for carrying out analysis of samples patent April 1999
Class characterization of circulating cancer cells isolated from body fluids and methods of use patent November 2005
Systems, devices, and methods for agglutination assays using sedimentation patent January 2016
Assay of blood or other biologic samples for target analytes patent June 1997
Method for detecting analytes in a sample patent-application December 2009
Toxin activity assays, devices, methods and systems therefor patent April 2016
Centrifuge with removable core for scalable centrifugation patent-application September 2005
Centrifugal hematology disposable patent November 2000
Microfluidics devices and methods for performing cell based assays patent-application August 2002
Microfluidic particle-analysis systems patent December 2007
Centrifugal Separation System patent-application August 2009
Heterogeneous binding assay patent April 1987
Monolithic microfluidic concentrators and mixers patent May 2005
Compact disk based platform for separating and detecting immunomagnetic bead labeled cells patent-application June 2010
Devices and Method For Using Centripetal Acceleration To Drive Fluid Movement In A Microfluidics System With On-Board Informatics patent-application December 2001
Method of separation employing magnetic particles and second medium patent January 1994
Systems and Methods for Handling Microfluidic Droplets patent-application October 2013
Assay system and method for conducting assays patent March 1999
Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation patent April 2016
Method Of Detecting Analyte-Molecule Interactions patent-application June 2008
Method For Isolating Nucleic Acids Comprising The Use Of Ethylene Glycol Multimers patent-application March 2009
Pre-column for preconcentrating substances to be chromatographed as well as device for a liquid chromatography system and coupling member for said device patent November 1985
Method for separating cells patent-application July 2003

Similar Records

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES