Devices, systems, and methods for detecting nucleic acids using sedimentation

Koh, Chung-Yan; Schaff, Ulrich Y.; Sommer, Gregory J.

Devices, systems, and methods for detecting nucleic acids using sedimentation

Patent · Tue Oct 24 04:00:00 EDT 2017

OSTI ID:1404945

Koh, Chung-Yan; Schaff, Ulrich Y.; Sommer, Gregory J.

Embodiments of the present invention are directed toward devices, systems, and method for conducting nucleic acid purification and quantification using sedimentation. In one example, a method includes generating complexes which bind to a plurality of beads in a fluid sample, individual ones of the complexes comprising a nucleic acid molecule such as DNA or RNA and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

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Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: National Technology & Engineering Solutions of Sandia, LLC

Patent Number(s):: 9,795,961

Application Number:: 13/423,008

OSTI ID:: 1404945

Country of Publication:: United States

Language:: English

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