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Title: Toxin activity assays, devices, methods and systems therefor

Abstract

Embodiments of the present invention are directed toward devices, system and method for conducting toxin activity assay using sedimentation. The toxin activity assay may include generating complexes which bind to a plurality of beads in a fluid sample. The complexes may include a target toxin and a labeling agent, or may be generated due to presence of active target toxin and/or labeling agent designed to be incorporated into complexes responsive to the presence of target active toxin. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a lower density than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

Inventors:
; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1245452
Patent Number(s):
9,304,128
Application Number:
14/157,278
Assignee:
Sandia Corporation (Albuquerque, NM)
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Jan 16
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory Jon. Toxin activity assays, devices, methods and systems therefor. United States: N. p., 2016. Web.
Koh, Chung-Yan, Schaff, Ulrich Y., & Sommer, Gregory Jon. Toxin activity assays, devices, methods and systems therefor. United States.
Koh, Chung-Yan, Schaff, Ulrich Y., and Sommer, Gregory Jon. Tue . "Toxin activity assays, devices, methods and systems therefor". United States. https://www.osti.gov/servlets/purl/1245452.
@article{osti_1245452,
title = {Toxin activity assays, devices, methods and systems therefor},
author = {Koh, Chung-Yan and Schaff, Ulrich Y. and Sommer, Gregory Jon},
abstractNote = {Embodiments of the present invention are directed toward devices, system and method for conducting toxin activity assay using sedimentation. The toxin activity assay may include generating complexes which bind to a plurality of beads in a fluid sample. The complexes may include a target toxin and a labeling agent, or may be generated due to presence of active target toxin and/or labeling agent designed to be incorporated into complexes responsive to the presence of target active toxin. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a lower density than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.},
doi = {},
url = {https://www.osti.gov/biblio/1245452}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {4}
}

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