Carbon-dependent chromate toxicity mechanism in an environmental Arthrobacter isolate

Field, Erin K.; Blaskovich, John P.; Peyton, Brent M.; Gerlach, Robin

doi:10.1016/j.jhazmat.2018.05.020

Carbon-dependent chromate toxicity mechanism in an environmental Arthrobacter isolate

Journal Article · Sat May 12 00:00:00 EDT 2018 · Journal of Hazardous Materials

DOI:https://doi.org/10.1016/j.jhazmat.2018.05.020· OSTI ID:1538458

Field, Erin K. ^[1]; Blaskovich, John P. ^[2]; Peyton, Brent M. ^[2]; Gerlach, Robin ^[2]

East Carolina University, Greenville, NC (United States); Montana State Univ., Bozeman, MT (United States); DOE/OSTI
Montana State Univ., Bozeman, MT (United States)

Arthrobacter spp. are widespread in soil systems and well-known for their Cr(VI) reduction capabilities making them attractive candidates for in situ bioremediation efforts. Cellulose drives carbon flow in soil systems; yet, most laboratory studies evaluate Arthrobacter-Cr(VI) interactions solely with nutrient-rich media or glucose. This work aims to determine how various cellulose degradation products and biostimulation substrates influence Cr(VI) toxicity, reduction, and microbial growth of an environmental Arthrobacter sp. isolate. Laboratory culture-based studies suggest there is a carbon-dependent Cr(VI) toxicity mechanism that affects subsequent Cr(VI) reduction by strain LLW01. Strain LLW01 could only grow in the presence of, and reduce, 50 μM Cr(VI) when glucose or lactate were provided. Compared to lactate, Cr(VI) was at least 30-fold and 10-fold more toxic when ethanol or butyrate was the sole carbon source, respectively. The addition of sulfate mitigated toxicity somewhat, but had no effect on the extent of Cr(VI) reduction. Cell viability studies indicated that a small fraction of cells were viable after 8 days suggesting cell growth and subsequent Cr(VI) reduction may resume. These results suggest when designing bioremediation strategies with Arthrobacter spp. such as strain LLW01, carbon sources such as glucose and lactate should be considered over ethanol and butyrate.

View Accepted Manuscript (DOE)

Research Organization:: Montana State Univ., Bozeman, MT (United States)

Sponsoring Organization:: USDOE Office of Science (SC); Montana University System Research Initiative

Grant/Contract Number:: FG02-06ER64206; AC07-05ID14517

OSTI ID:: 1538458

Alternate ID(s):: OSTI ID: 1601024
OSTI ID: 22789064

Journal Information:: Journal of Hazardous Materials, Journal Name: Journal of Hazardous Materials Journal Issue: C Vol. 355; ISSN 0304-3894

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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54 ENVIRONMENTAL SCIENCES
Arthrobacter
Bioremediation
Carbon
Engineering
Environmental Sciences & Ecology
Hexavalent chromium
Low-level waste

Carbon-dependent chromate toxicity mechanism in an environmental Arthrobacter isolate

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References (46)

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