Direct and Indirect Reduction of Cr(VI) by Fermentative Fe(III)-Reducing Cellulomonas sp. Strain Cellu-2a

Khanal, Anamika; Hur, Hor-Gil; Fredrickson, James K.; Lee, Ji-Hoon

doi:10.4014/jmb.2107.07038

Direct and Indirect Reduction of Cr(VI) by Fermentative Fe(III)-Reducing Cellulomonas sp. Strain Cellu-2a

Journal Article · Sat Nov 27 23:00:00 EST 2021 · Journal of Microbiology and Biotechnology (Print)

DOI:https://doi.org/10.4014/jmb.2107.07038· OSTI ID:2471582

Khanal, Anamika ^[1]; Hur, Hor-Gil ^[2]; Fredrickson, James K. ^[3]; Lee, Ji-Hoon ^[1]

Jeonbuk National University (Korea, Republic of)
Gwangju Institute of Science and Technology (Korea, Republic of)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Hexavalent chromium (Cr(VI)) is recognized to be carcinogenic and toxic and registered as a contaminant in many drinking water regulations. It occurs naturally and is also produced by industrial processes. The reduction of Cr(VI) to Cr(III) has been a central topic for chromium remediation since Cr(III) is less toxic and less mobile. In this study, fermentative Fe(III)-reducing bacterial strains (Cellu-2a, Cellu-5a, and Cellu-5b) were isolated from a groundwater sample and were phylogenetically related to species of Cellulomonas by 16S rRNA gene analysis. One selected strain, Cellu-2a showed its capacity of reduction of both soluble iron (ferric citrate) and solid iron (hydrous ferric oxide, HFO), as well as aqueous Cr(VI). The strain Cellu-2a was able to reduce 15 μM Cr(VI) directly with glucose or sucrose as a sole carbon source under the anaerobic condition and indirectly with one of the substrates and HFO in the same incubations. The heterogeneous reduction of Cr(VI) by the surface-associated reduced iron from HFO by Cellu-2a likely assisted the Cr(VI) reduction. Fermentative features such as large-scale cell growth may impose advantages on the application of bacterial Cr(VI) reduction over anaerobic respiratory reduction.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 2471582

Journal Information:: Journal of Microbiology and Biotechnology (Print), Journal Name: Journal of Microbiology and Biotechnology (Print) Journal Issue: 11 Vol. 31; ISSN 1017-7825

Publisher:: Korean Society for Microbiology and Biotechnology (KMB)Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Direct and Indirect Reduction of Cr(VI) by Fermentative Fe(III)-Reducing Cellulomonas sp. Strain Cellu-2a

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