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Transgenic cells with increased plastoquinone levels and methods of use

Patent ·
OSTI ID:1496861

Disclosed herein are transgenic cells expressing a heterologous nucleic acid encoding a prephenate dehydrogenase (PDH) protein, a heterologous nucleic acid encoding a homogentisate solanesyl transferase (HST) protein, a heterologous nucleic acid encoding a deoxyxylulose phosphate synthase (DXS) protein, or a combination of two or more thereof. In particular examples, the disclosed transgenic cells have increased plastoquinone levels. Also disclosed are methods of increasing cell growth rates or production of biomass by cultivating transgenic cells expressing a heterologous nucleic acid encoding a PDH protein, a heterologous nucleic acid encoding an HST protein, a heterologous nucleic acid encoding a DXS protein, or a combination of two or more thereof under conditions sufficient to produce cell growth or biomass.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC52-06NA25396
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Number(s):
10,106,809
Application Number:
15/389,061
OSTI ID:
1496861
Country of Publication:
United States
Language:
English

References (7)

Optimization of photosynthetic light energy utilization by microalgae journal October 2012
The pds2 mutation is a lesion in the Arabidopsis homogentisate solanesyltransferase gene involved in plastoquinone biosynthesis journal June 2007
Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana journal September 2002
Constitutive overexpression of barley 4-hydroxyphenylpyruvate dioxygenase in tobacco results in elevation of the vitamin E content in seeds but not in leaves journal March 2003
Engineering Plant Shikimate Pathway for Production of Tocotrienol and Improving Herbicide Resistance journal December 2003
Characterization of homogentisate prenyltransferases involved in plastoquinone-9 and tocochromanol biosynthesis journal September 2006
Increasing anthraquinone production by overexpression of 1-deoxy-d-xylulose-5-phosphate synthase in transgenic cell suspension cultures of Morinda citrifolia journal March 2010

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