skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Compositions and methods relating to transgenic plants and cellulosic ethanol production

Patent ·
OSTI ID:1183450

Transgenic lignocellulosic plants are provided according to embodiments of the present invention, the transgenic plants transformed with an expression cassette encoding a protein operably linked to a signal peptide which targets the protein to a cell wall of the transgenic plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine. Methods of increasing lignin-protein bonds in a lignocellulosic plant are provided according to embodiments of the present invention which include expressing a recombinant nucleic acid in a lignocellulosic plant, the recombinant nucleic acid encoding a protein operably linked to a signal peptide which targets the protein to the cell wall of a plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine.

Research Organization:
The Penn State Research Foundation, University Park, PA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-07ER15891
Assignee:
The Penn State Research Foundation (University Park, PA)
Patent Number(s):
9,045,764
Application Number:
13/435,761
OSTI ID:
1183450
Country of Publication:
United States
Language:
English

References (21)

Method of altering lignin in trees patent October 1998
Coniferin beta-glucosidase cDNA for modifying lignin content in plants patent October 1999
Process for obtaining fungal resistant plants with recombinant polynucleotides encoding .beta.-1,3-glucanase modified for apoplast targeting patent May 2000
High lysine fertile transgenic corn plants patent December 2001
Materials and methods for the modification of plant lignin content patent June 2002
Modification of lignin content and composition in plants patent August 2002
Genes encoding p-coumarate 3-hydroxylase (C3H) and methods of use patent July 2006
Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars patent-application September 2002
Methods and genetic constructs for modification of lignin composition of corn cobs patent-application November 2006
cDNA cloning and heterologous expression of coniferin β-glucosidase journal January 1999
Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase journal November 2001
Di-isodityrosine Is the Intermolecular Cross-link of Isodityrosine-rich Extensin Analogs Cross-linked in Vitro journal October 2004
Signal Peptide-Dependent Targeting of a Rice α -Amylase and Cargo Proteins to Plastids and Extracellular Compartments of Plant Cells journal July 2004
Predicting Subcellular Localization of Proteins Based on their N-terminal Amino Acid Sequence journal July 2000
Structure and function of plant cell wall proteins. journal January 1993
Plant γ-thionins: Novel insights on the mechanism of action of a multi-functional class of defense proteins journal November 2005
Improved Prediction of Signal Peptides: SignalP 3.0 journal July 2004
Plant cell wall Proteins journal June 1998
Glycine-rich proteins as structural components of plant cell walls journal September 2001
Tetramethylammonium Hydroxide (TMAH) Thermochemolysis of Lignin:  Behavior of 4- O -Etherified Cinnamyl Alcohols and Aldehydes journal November 2005
The energetics of ion-pair and hydrogen-bonding interactions in a helical peptide journal September 1993