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Compositions and methods relating to transgenic plants and cellulosic ethanol production

Patent ·
OSTI ID:1040006
 [1];  [2];  [3]
  1. State College, PA
  2. Port Matilda, PA
  3. Clemson, SC
+ Show Author Affiliations
Transgenic lignocellulosic plants are provided according to embodiments of the present invention, the transgenic plants transformed with an expression cassette encoding a protein operably linked to a signal peptide which targets the protein to a cell wall of the transgenic plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine. Methods of increasing lignin-protein bonds in a lignocellulosic plant are provided according to embodiments of the present invention which include expressing a recombinant nucleic acid in a lignocellulosic plant, the recombinant nucleic acid encoding a protein operably linked to a signal peptide which targets the protein to the cell wall of a plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine.
Research Organization:
Penn State Univ., University Park, PA (United States)
Sponsoring Organization:
USDOE
Assignee:
The Penn State Research Foundation (University Park, PA)
Patent Number(s):
8,163,976
Application Number:
12/126,569
OSTI ID:
1040006
Country of Publication:
United States
Language:
English

References (13)

Predicting Subcellular Localization of Proteins Based on their N-terminal Amino Acid Sequence journal July 2000
Di-isodityrosine Is the Intermolecular Cross-link of Isodityrosine-rich Extensin Analogs Cross-linked in Vitro journal October 2004
Tetramethylammonium Hydroxide (TMAH) Thermochemolysis of Lignin:  Behavior of 4- O -Etherified Cinnamyl Alcohols and Aldehydes journal November 2005
Structure and function of plant cell wall proteins. journal January 1993
Glycine-rich cell wall proteins in bean: gene structure and association of the protein with the vascular system. journal December 1988
The energetics of ion-pair and hydrogen-bonding interactions in a helical peptide journal September 1993
Signal Peptide-Dependent Targeting of a Rice α -Amylase and Cargo Proteins to Plastids and Extracellular Compartments of Plant Cells journal July 2004
Plant cell wall Proteins journal June 1998
cDNA cloning and heterologous expression of coniferin β-glucosidase journal January 1999
Plant γ-thionins: Novel insights on the mechanism of action of a multi-functional class of defense proteins journal November 2005
Glycine-rich proteins as structural components of plant cell walls journal September 2001
Improved Prediction of Signal Peptides: SignalP 3.0 journal July 2004
Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase journal November 2001

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59 BASIC BIOLOGICAL SCIENCES