Human white blood cells contain cyclobutyl pyrimidine dimer photolyase
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America
- Brookhaven National Lab., Upton, NY (United States)
Although enzymatic photoreactivation of cyclobutyl pyrimidine dimers in DNA is present in almost all organisms, its presence in placental mammals is controversial. We tested human white blood cells for photolyase by using three defined DNAs (suprecoiled pET-2, nonsupercoiled bacteriphage {lambda}, and a defined-sequence 287-bp oligonucleotide), two dimer-specific endonucleases (T4 endonuclease V and UV endonuclease from Micrococcus luteus), and three assay methods. We show that human white blood cells contain photolyase that can photorepair pyrimidine dimers in defined supercoiled and linear DNAs and in a 287-bp oligonucleotide and that human photolyase is active on genomic DNA in intact human cells. 44 refs., 3 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 146517
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 21 Vol. 92; ISSN 0027-8424; ISSN PNASA6
- Country of Publication:
- United States
- Language:
- English
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