Substrate specificity of Micrococcus luteus uv endonuclease and its overlap with DNA photolyase activity
Conference
·
OSTI ID:4144811
- ed.
The action of an endonuclease from Micrococcus luteus that operates on uv damage in DNA overlaps with that of DNA photolyase from yeast: homo- and heterocyclobutane dipyrimidines in DNA are substrates for both enzymes, but pyrimidine adducts or the spore photoproduct in DNA are not. As expected from this overlap, the action of the two enzymes is mutually interfering: single- strand nicks introduced by the endonuclease effectively preclude photoreactivation; conversely, formation of a photolyase-cyclobutane dipyrimidine complex can prevent nicking by the endonuclease. (auth)
- Research Organization:
- Univ. of Texas, Dallas
- NSA Number:
- NSA-33-012269
- OSTI ID:
- 4144811
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Journal Article
·
Mon Oct 01 00:00:00 EDT 1973
· Genetika, v. 9, no. 10, pp. 97-105
·
OSTI ID:4338966
Comparison of the cleavage of pyrimidine dimers by the bacteriophage T4 and Micrococcus luteus uv-specific endonucleases
Journal Article
·
Wed Dec 24 23:00:00 EST 1980
· J. Biol. Chem.; (United States)
·
OSTI ID:6055809
Selective inhibition by methoxyamine of the apurinic/apyrimidinic endonuclease activity associated with pyrimidine dimer-DNA glycosylases from Micrococcus luteus and bacteriophage T4
Journal Article
·
Tue Jun 16 00:00:00 EDT 1987
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·
OSTI ID:6368608
Related Subjects
*DNA-- BIOLOGICAL RADIATION EFFECTS
*LYASES-- METABOLISM
*MICROCOCCUS-- BIOLOGICAL RADIATION EFFECTS
*NUCLEASES-- METABOLISM
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
CATALYSIS
N48110 --Life Sciences--Radiation Effects on Biochemicals--In-Vitro
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PYRIMIDINES
ULTRAVIOLET RADIATION
YEASTS
*LYASES-- METABOLISM
*MICROCOCCUS-- BIOLOGICAL RADIATION EFFECTS
*NUCLEASES-- METABOLISM
560131* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Microorganisms--Basic Studies
CATALYSIS
N48110 --Life Sciences--Radiation Effects on Biochemicals--In-Vitro
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PYRIMIDINES
ULTRAVIOLET RADIATION
YEASTS