Quantitative detection of pathogens in centrifugal microfluidic disks

Koh, Chung-Yan; Schaff, Ulrich Y.; Sommer, Gregory Jon

Quantitative detection of pathogens in centrifugal microfluidic disks

Patent · 27 February 2018

OSTI ID:1423444

Koh, Chung-Yan; Schaff, Ulrich Y.; Sommer, Gregory Jon

A system and methods for detection of a nucleic acid including forming a plurality of nucleic acid detection complexes are described, each of the complexes including a nucleic acid analyte, a detection agent and a functionalized probe. The method further including binding the nucleic acid detection complexes to a plurality of functionalized particles in a fluid sample and separating the functionalized particles having the nucleic acid detection complexes bound thereto from the fluid sample using a density media. The nucleic acid analyte is detected by detecting the detection agent.

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Research Organization:: Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000;

Assignee:: National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)

Patent Number(s):: 9,903,001

Application Number:: 13/941,186

OSTI ID:: 1423444

Country of Publication:: United States

Language:: English

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