Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock

Bagnoud, Alexandre; Chourey, Karuna; Hettich, Robert L.; de Bruijn, Ino; Andersson, Anders F.; Leupin, Olivier X.; Schwyn, Bernhard; Bernier-Latmani, Rizlan

doi:10.1038/ncomms12770

Title: Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock

Journal Article · Fri Oct 14 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms12770· OSTI ID:1352739

Bagnoud, Alexandre ^[1]; Chourey, Karuna ^[2]; Hettich, Robert L. ^[2];

^[3];

^[4]; Leupin, Olivier X. ^[5]; Schwyn, Bernhard ^[5]; Bernier-Latmani, Rizlan ^[1]

Ecole Polytechnique Federale Lausanne (Switzlerland). Environmental Microbiology Lab.
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
Bioinformatics Infrastructure for Life Sciences (BILS), Stockholm (Sweden); KTH Royal Inst. of Technology, Stockholm (Sweden). School of Biotechnology, Division of Gene Technology, Science for Life Lab.
KTH Royal Inst. of Technology, Stockholm (Sweden). School of Biotechnology, Division of Gene Technology, Science for Life Lab.
National Cooperative for the Disposal of Radioactive Waste (Nagra), Wettingen (Switzerland)

A significant fraction (~ 20%) of microbial life is found in the terrestrial deep subsurface, yet the metabolic processes extant in those environments are poorly understood. Here we show that H₂, injected into the Opalinus Clay formation in a borehole located 300 meters below the surface, fuels a community of microorganisms with interconnected metabolisms. Metagenomic binning and metaproteomic analysis reveal a complete carbon cycle, driven by autotrophic hydrogen oxidizers. Dead biomass from these organisms is a substrate for a fermenting bacterium that produces acetate as a product. In turn, complete oxidizer heterotrophic sulfate- reducing bacteria utilize acetate and oxidize it to CO₂, closing the cycle. This metabolic reconstruction sheds light onto a hydrogen-driven carbon cycle, and a sunlight-independent ecosystem in the deep subsurface.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC); Swedish Research Council (SRC)

Grant/Contract Number:: AC05-00OR22725; AC02-05CH11231; CSP 1505; 2011-5689

OSTI ID:: 1352739

Journal Information:: Nature Communications, Vol. 7, Issue 10; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 64 works

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Title: Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock

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