Metagenomic analysis of a high carbon dioxide subsurface microbial community populated by chemolithoautotrophs and bacteria and archaea from candidate phyla

Emerson, Joanne B.; Thomas, Brian C.; Alvarez, Walter; Banfield, Jillian F.

doi:10.1111/1462-2920.12817

Metagenomic analysis of a high carbon dioxide subsurface microbial community populated by chemolithoautotrophs and bacteria and archaea from candidate phyla

Journal Article · Sat Feb 28 00:00:00 EST 2015 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.12817· OSTI ID:1571927

Emerson, Joanne B. ^[1]; Thomas, Brian C. ^[1]; Alvarez, Walter ^[1]; Banfield, Jillian F. ^[1]

Univ. of California, Berkeley, CA (United States)

Research on geologic carbon sequestration raises questions about potential impacts of subsurface microbiota on carbon cycling and biogeochemistry. Subsurface, high-CO₂ systems are poorly biologically characterized, partly because of difficulty accessing high-volume, uncontaminated samples. CO₂-driven Crystal Geyser (CG, Utah, USA), an established geologic carbon sequestration analogue, provides high volumes of deep (~200-500 m) subsurface fluids. We explored microbial diversity and metabolic potential in this high-CO₂ environment by assembly and analysis of metagenomes recovered from geyser water filtrate. The system is dominated by neutrophilic, iron-oxidizing bacteria, including 'marine' Mariprofundus (Zetaproteobacteria) and 'freshwater' Gallionellales, sulfur-oxidizing Thiomicrospira crunogena and Thiobacillus-like Hydrogenophilales. Near-complete genomes were reconstructed for these bacteria. CG is notably populated by a wide diversity of bacteria and archaea from phyla lacking isolated representatives (candidate phyla) and from as-yet undefined lineages. Many bacteria affiliate with OD1, OP3, OP9, PER, ACD58, WWE3, BD1-5, OP11, TM7 and ZB2. The recovery of nearly 100 genes encoding ribulose-1,5 bisphosphate carboxylase-oxygenase subunit proteins of the Calvin cycle and AMP salvage pathways suggests a strong biological role in high-CO₂ subsurface carbon cycling. Altogether, we predict microbial impacts on subsurface biogeochemistry via iron, sulfur, and complex carbon oxidation, carbon and nitrogen fixation, fermentation, hydrogen metabolism, and aerobic and anaerobic respiration.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1571927

Alternate ID(s):: OSTI ID: 1370614
OSTI ID: 1387294

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 6 Vol. 18; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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New Insight into Microbial Iron Oxidation as Revealed by the Proteomic Profile of an Obligate Iron-Oxidizing Chemolithoautotroph Barco, Roman A.; Emerson, David; Sylvan, Jason B. Applied and Environmental Microbiology, Vol. 81, Issue 17 https://doi.org/10.1128/aem.01374-15	journal	June 2015
Insights into Carbon Metabolism Provided by Fluorescence In Situ Hybridization-Secondary Ion Mass Spectrometry Imaging of an Autotrophic, Nitrate-Reducing, Fe(II)-Oxidizing Enrichment Culture Tominski, Claudia; Lösekann-Behrens, Tina; Ruecker, Alexander Applied and Environmental Microbiology, Vol. 84, Issue 9 https://doi.org/10.1128/aem.02166-17	journal	March 2018
Growth and Population Dynamics of the Anaerobic Fe(II)-Oxidizing and Nitrate-Reducing Enrichment Culture KS Tominski, Claudia; Heyer, Helene; Lösekann-Behrens, Tina Applied and Environmental Microbiology, Vol. 84, Issue 9 https://doi.org/10.1128/aem.02173-17	journal	March 2018
Microbial Surface Colonization and Biofilm Development in Marine Environments Dang, Hongyue; Lovell, Charles R. Microbiology and Molecular Biology Reviews, Vol. 80, Issue 1 https://doi.org/10.1128/mmbr.00037-15	journal	December 2015

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