Analysis of apoB and apoC-II gene polymorphism in random sample and CHD patients from Moscow
- Institute of Molecular Genetics, Moscow (Russian Federation); and others
We have analyzed the allele frequency distributions of the 3{prime} apoB gene minisatellite and apoC-II gene microsatellite in random sample of coronary heart disease (CHD) patients. For this purpose we used the PCR technique followed by high-resolution PAGE. It was revealed that the apoB allele, harboring 30 repeats (apoB 30), as well as the apoC-II allele harboring 30 repeats (apoC-II 30), were less frequent in patients at the same time as the frequency of the apoB 32 and apoC-II 17 alleles was greater in patients. The greater frequency of apoB alleles which were larger in size than apoB 46 (defined as `long` - L) was observed in patients with high apoB levels (>160mg dl). The analysis of apoB genotype distribution showed that in a random sample the most common genotype was apoB 34,36 (a combination of the most frequent alleles in the random sample). In patients with high apoB levels, it was twice less frequent, and the most common genotype was apoB 36,L (43% versus 12% in the random sample). Analysis of data on a lipid spectrum of subjects from the random sample with different apoB and apoC-II 17 alleles were associated with atherogenic shifts in the lipid profile, at the same time as apoB 30 and apoC-II 30 alleles - with an apparently favorable lipid profile. The increment of the disease-related risk was observed for subjects with a combination of apoB 32 allele or apoB 36,L genotype with the apoC-11 17 allele. Alternatively, combination of these apoB variants with the apoC-II 30 allele resulted in decreased related risk. In conclusion, simultaneous analysis of two candidate gene variants demonstrated interaction in their influence on the lipid spectrum.
- OSTI ID:
- 134766
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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