Localization of the chromosome 11 breakpoint in a translocation t(4;11)(q21;p15) in T cell acute lymphocytic leukemia (T-ALL)
- Queen Elizabeth Hospital, Woodville (Australia); and others
The identification of genes located at the breakpoints of chromosomal translocations has been invaluable in determining the pathogenesis of hematogical malignancies. We have investigated the translocation, t(4;11)(q21;p14-15) in a T-ALL patient. Four cases with a similar translocation have been reported; three at diagnosis and one as a secondary cytogenetic change. As a first step towards cloning of the breakpoint sequences, we isolated a somatic cell hybrid containing the der(4) translocation chromosome. The hybrid allowed us to determine the markers flanking the chromosome 11 breakpoint position. The breakpoint was localized to band 11p15.5 between the genes for interferon-like growth factor II (IGF2) and the M1 subunit of ribonucleotide reductase (RRM1) by PCR. Cosmid probes from this region were then used to further localize the breakpoint to a region adjacent to RRM1 by fluorescent in situ hybridization (FISH).
- OSTI ID:
- 134521
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mapping chromosome band 11q23 in human acute leukemia with biotinylated probes: Identification of 11q23 translocation breakpoints with a yeast artificial chromosome
Identification of a gene, MLL, that spans the breakpoint in 11q23 translocations associated with human leukemias