Proximal chromosome 3p harbors a DNA segment frequently deleted in small cell lung cancer
- Eleanor Roosevelt Institute, Denver, CO (United States); and others
Several lines of evidence suggest that potential tumor suppressor genes involved in small cell lung cancer (SCLC) reside at three separate locations on human chromosome 3p including 3p25, 3p21.3 and 3p14-cen. The most proximal region (3p14-cen) was first identified by a homozygous deletion of 7-10 Mb found in a SCLC cell line U2020. Daly et al. (1991) reported allele loss in a tumor sample from the 3p14-cen region in an SCLC tumor sample which retained heterozygosity distally and this same segment has been implicated in both sporadic and familial kidney cancer. Other evidence suggests that deletion of this proximal region may be an early event in SCLC development. Our analysis of 31 SCLC cell lines with 26 microsatellite repeat DNA markers (most of which fall into the region 3p14-cen) indicates that the majority of SCLC samples lose an entire copy of 3p. However, from those cell lines which retain distal heterozygosity, there is evidence for potential regions of loss in 3p13 and 3p14. Analysis of matched pairs of normal/tumor samples from SCLC patients has confirmed nearly complete loss of 3p in most cases and has provided further evidence of loss in the region 3p14-cen. All samples that retain heterozygosity at the distal markers are being analyzed with the complete set of 3p markers in order to refine the location of any potential tumor suppressor gene(s). This information along with the DNA contig physical map we have constructed of this portion of the chromosome should allow rapid isolation of potential tumor suppressor loci involved in SCLC and possibly other forms of cancer.
- OSTI ID:
- 133586
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-0315
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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