YAC STS-content maps of human chromosome 4
- Stanford Univ., CA (United States); and others
We are using human chromosome 4 as a model system for constructing yeast artificial chromosome STS-content map of the human genome. We have used a large collection of STSs distributed throughout the chromosome in a three-tiered pooling scheme to screen portions of the CEPH Mark I and Mega YAC libraries with PCR assays for each of the STSs. We applied an adaptation of the computer program SEGMAP to determine STS order and to establish YAC contigs. By using large numbers of STSs to screen YACs comprising about eight genome equivalents, we have found that maps covering most of the chromosome with almost complete continuity can be built solely by STS-content analysis with a minimum amount of YAC end isolation and analysis. In cases where gaps remain, the orders and orientations of the YAC/STS contigs are determined by radiation hybrid and meiotic mapping.To date, we have used this approach to complete an STS-content YAC map of the short arm of human chromosome 4, which is estimated to be about 50 Mb in length, and are nearing completion of a similar map for the 150 Mb long arm. The 4p map contains over 600 YACs of average length of 675 kb, and contains four gaps, all of which are clustered in the distal eight Mb of 4p. We estimate that the cloned coverage in this map is a least 96% of the short arm. The map contains 379 STSs, 318 of which can be ordered relative to each other by STS-content analysis of the YACs, resulting in an average resolution of 160 kb. This map is available from our ftp server (toolik.stanford.edu). In addition to the 4p map, we have completed screening the YAC libraries with over 900 STSs from the long arm of the chromosome, and are currently using SEGMAP to build a map of 4q.
- OSTI ID:
- 133347
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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