A verified minimal YAC contig for human chromosome 21
Journal Article
·
· American Journal of Human Genetics
OSTI ID:134796
- Eleanor Roosevelt Institute, Denver, CO (United States); and others
The goal of this project is the construction of a verified YAC contig of the complete long arm of human chromosome 21 utilizing YACs from the CEPH and St. Louis libraries. The YACs in this contig have been analyzed for size by PFGE, tested for chimerism by FISH or end-cloning, and verified for STS content by PCR. This last analysis has revealed a number of cases of conflict with the published STS order. To establish correct order, we have utilized STS content analysis of somatic cell hybrids containing portions of chromosome 21. Additional problems being addressed include completeness of coverage and possible deletions or gaps. Questions of completeness of the CEPH 810 YAC set arose after screening with 57 independently derived probes failed to identify clones for 11 (19%). Ten of the 11, however, do detect chromosome 21 cosmids when used to screen Lawrence Livermore library LL21NC02`G,` a cosmid library constructed from flow-sorted chromosomes 21. Remaining gaps in the contig are being closed by several methods. These include YAC fingerprinting and conversion of YACs to cosmids. In addition, we are establishing the overlap between the physical NotI map and the YAC contig by testing YACs for NotI sites and screening the YACs in the contig for the presence of NotI-linking clones.
- OSTI ID:
- 134796
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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