Towards an STS-content map of the human genome
- Massachusetts Institute of Technology, Cambridge, MA (United States); and others
The principal goal of the Human Genome Mapping Project is the construction of an STS content map of the human genome - consisting of 10,000 STSs screened on the CEPH mega-YAC library, in order to identify the YACs containing each STS. Over the past year, we have been developing methods to scale up physical mapping, which includes a semi-automated PCR setup process, detection of the products by a chemiluminescent hybridization assay, and data capture using CCD cameras. To date, we have identified YACs for 3106 STSs which fall into the following categories: (i) 1376 genetically mapped polymorphic STSs, which allow contigs to be anchored to the genetic map; (ii) 565 random genome-wide STSs, of which approximately 75% are unambiguously assigned to a chromosome using the NIGMS Human/Rodent Somatic Cell Hybrid Mapping Panel No. 1; (iii) 918 unpublished CA-repeat-containing STSs generously provided by J. Weissenbach (iv) 98 new chromosome 22 STSs generated from sequences derived from flow-sorted chromosome libraries, in collaboration with the Human Genome Center for Chromosome 22 in Philadelphia; these STSs and the corresponding YACs already provide considerable coverage of the long arm of chromosome 22; (v) and 149 STSs from public data bases. STS-content data generated at the Center is made accessible by quarterly data releases which are accessible by ftp to genome.wi.mit.edu, directory `/distribution/human-STS-releases`.
- OSTI ID:
- 133348
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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