Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Chromy, Brett A.; Henderson, Paul; Hoeprich, Jr, Paul D.

Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Patent · Tue Oct 04 04:00:00 EDT 2016

OSTI ID:1327989

Chromy, Brett A.; Henderson, Paul; Hoeprich, Jr, Paul D.

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

View Patent

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC52-07NA27344

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Number(s):: 9,458,191

Application Number:: 14/536,513

OSTI ID:: 1327989

Country of Publication:: United States

Language:: English

References (121)

Overview of Protein Structural and Functional Folds Sun, Peter D.; Foster, Christine E.; Boyington, Jeffrey C. Current Protocols in Protein Science, Vol. 35, p. 17.1.1.1–17.1.189 https://doi.org/10.1002/0471140864.ps1701s35	book	February 2004
Predictable difficulty or difficulty to predict: Letter to the Editor Arányi, Tamás; Fülöp, Krisztina; Symmons, Orsolya Protein Science, Vol. 20, Issue 1 https://doi.org/10.1002/pro.552	journal	December 2010
Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms Wallin, Erik; Heijne, Gunnar Von Protein Science, Vol. 7, Issue 4 https://doi.org/10.1002/pro.5560070420	journal	April 1998
Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli Hill, Margaret A.; Preiss, Jack Biochemical and Biophysical Research Communications, Vol. 244, Issue 2, p. 573-577 https://doi.org/10.1006/bbrc.1998.8301	journal	March 1998
Two-Dimensional Crystallization of Histidine-Tagged, HIV-1 Reverse Transcriptase Promoted by a Novel Nickel-Chelating Lipid Kubalek, Elizabeth W.; Le Grice, Stuart F. J.; Brown, Patrick O. Journal of Structural Biology, Vol. 113, Issue 2 https://doi.org/10.1006/jsbi.1994.1039	journal	September 1994
Ab Initio Protein Structure Prediction Lee, Jooyoung; Wu, Sitao; Zhang, Yang From Protein Structure to Function with Bioinformatics https://doi.org/10.1007/978-1-4020-9058-5_1	book	January 2009
Development of Crops to Produce Industrially Useful Natural Rubber Whalen, Maureen; McMahan, Colleen; Shintani, David Isoprenoid Synthesis in Plants and Microorganisms, p. 329-345 https://doi.org/10.1007/978-1-4614-4063-5_23	book	January 2012
Stability of pressure-extruded liposomes made from archaeobacterial ether lipids Choquet, C. G.; Patel, G. B.; Sprott, G. D. Applied Microbiology and Biotechnology, Vol. 42, Issue 2-3, p. 375-384 https://doi.org/10.1007/BF00902745	journal	November 1994
The Genetic Algorithm Approach to Protein Structure Prediction Unger, Ron Structure and Bonding, Vol. v. 110, p. 153-175 https://doi.org/10.1007/b13936	book	February 2004
High-Density Universal 16S rRNA Microarray Analysis Reveals Broader Diversity than Typical Clone Library When Sampling the Environment DeSantis, Todd Z.; Brodie, Eoin L.; Moberg, Jordan P. Microbial Ecology, Vol. 53, Issue 3 https://doi.org/10.1007/s00248-006-9134-9	journal	March 2007
Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems Terpe, K. Applied Microbiology and Biotechnology, Vol. 60, Issue 5, p. 523-533 https://doi.org/10.1007/s00253-002-1158-6	journal	January 2003
Disentangling the perturbational effects of amino acid substitutions in the DNA-binding domain of p53 Wacey, A. I.; Cooper, D. N.; Liney, D. Human Genetics, Vol. 104, Issue 1, p. 15-22 https://doi.org/10.1007/s004390050904	journal	February 1999
Kinetic properties of recombinant MAO-A on incorporation into phospholipid nanodisks Cruz, F.; Edmondson, D. E. Journal of Neural Transmission, Vol. 114, Issue 6 https://doi.org/10.1007/s00702-007-0673-0	journal	March 2007
Characterization of a HoxEFUYH type of [NiFe] hydrogenase from Allochromatium vinosum and some EPR and IR properties of the hydrogenase module Long, Minnan; Liu, Jingjing; Chen, Zhifeng JBIC Journal of Biological Inorganic Chemistry, Vol. 12, Issue 1 https://doi.org/10.1007/s00775-006-0162-1	journal	September 2006
Preparation and Characterization of Nickel Nanoparticles for Binding to His-tag Proteins and Antigens Patel, Jigna D.; O’Carra, Ronan; Jones, Julia Pharmaceutical Research, Vol. 24, Issue 2 https://doi.org/10.1007/s11095-006-9154-7	journal	December 2006
Specific targeting of a lipophilic prodrug of iododeoxyuridine to parenchymal liver cells using lactosylated reconstituted high density lipoprotein particles Bijsterbosch, Martin K.; van de Bilt, Hendrika; van Berkel, Theo J. C. Biochemical Pharmacology, Vol. 52, Issue 1, p. 113-121 https://doi.org/10.1016/0006-2952(96)00170-0	journal	July 1996
Adjuvants for human vaccines—current status, problems and future prospects Gupta, Rajesh K.; Siber, George R. Vaccine, Vol. 13, Issue 14, p. 1263-1276 https://doi.org/10.1016/0264-410X(95)00011-O	journal	January 1995
Natural Rubber Biosynthesis in Plants Cornish, Katrina; Xie, Wenshuang Methods in Enzymology, Vol. 515, p. 63-82 https://doi.org/10.1016/B978-0-12-394290-6.00004-5	book	January 2012
HoxE—a subunit specific for the pentameric bidirectional hydrogenase complex (HoxEFUYH) of cyanobacteria Schmitz, Oliver; Boison, Gudrun; Salzmann, Heike Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1554, Issue 1-2, p. 66-74 https://doi.org/10.1016/S0005-2728(02)00214-1	journal	April 2002
Attaching histidine-tagged peptides and proteins to lipid-based carriers through use of metal-ion-chelating lipids Chikh, Ghania G.; Li, Wai Ming; Schutze-Redelmeier, Marie-Paule Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1567, p. 204-212 https://doi.org/10.1016/S0005-2736(02)00618-1	journal	December 2002
Osmotic shrinkage of giant egg-lecithin vesicles Boroske, E.; Elwenspoek, M.; Helfrich, W. Biophysical Journal, Vol. 34, Issue 1, p. 95-109 https://doi.org/10.1016/S0006-3495(81)84839-4	journal	April 1981
Specific Interaction and Two-Dimensional Crystallization of Histidine Tagged Yeast RNA Polymerase I on Nickel-Chelating Lipids Bischler, Nicolas; Balavoine, Fabrice; Milkereit, Philipp Biophysical Journal, Vol. 74, Issue 3, p. 1522-1532 https://doi.org/10.1016/S0006-3495(98)77864-6	journal	March 1998
Surface changes induced by osmotic shrinkage on large unilamellar vesicles Disalvo, E. A.; Campos, A. M.; Abuin, E. Chemistry and Physics of Lipids, Vol. 84, Issue 1, p. 35-45 https://doi.org/10.1016/S0009-3084(96)02617-5	journal	November 1996
A bilayer cell-free protein synthesis system for high-throughput screening of gene products Sawasaki, Tatsuya; Hasegawa, Yoshinori; Tsuchimochi, Masateru FEBS Letters, Vol. 514, Issue 1, p. 102-105 https://doi.org/10.1016/S0014-5793(02)02329-3	journal	February 2002
Phospholipid phase transitions in homogeneous nanometer scale bilayer discs Shaw, Andrew W.; McLean, Mark A.; Sligar, Stephen G. FEBS Letters, Vol. 556, Issue 1-3, p. 260-264 https://doi.org/10.1016/S0014-5793(03)01400-5	journal	December 2003
Using Nanodiscs to Create Water‐Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers Boldog, Thomas; Li, Mingshan; Hazelbauer, Gerald L. Methods in Enzymology, Vol. 423, p. 317-335 https://doi.org/10.1016/S0076-6879(07)23014-9	book	January 2007
Protein Translocons Schnell, Danny J.; Hebert, Daniel N. Cell, Vol. 112, Issue 4 https://doi.org/10.1016/S0092-8674(03)00110-7	journal	February 2003
Novel applications of liposomes Lasic, D. Trends in Biotechnology, Vol. 16, Issue 7 https://doi.org/10.1016/S0167-7799(98)01220-7	journal	July 1998
Recombinant lipoproteins: lipoprotein-like lipid particles for drug targeting Rensen, Patrick C. N.; de Vrueh, Remco L. A.; Kuiper, Johan Advanced Drug Delivery Reviews, Vol. 47, Issue 2-3, p. 251-276 https://doi.org/10.1016/S0169-409X(01)00109-0	journal	April 2001
The Biosynthesis of Natural Rubber Paterson-Jones, J. C.; Gilliland, M. G.; Van Staden, J. Journal of Plant Physiology, Vol. 136, Issue 3, p. 257-263 https://doi.org/10.1016/S0176-1617(11)80047-7	journal	June 1990
CpG DNA induces stronger immune responses with less toxicity than other adjuvants Weeratna, Risini D.; McCluskie, Michael J.; Xu, Yu Vaccine, Vol. 18, Issue 17, p. 1755-1762 https://doi.org/10.1016/S0264-410X(99)00526-5	journal	March 2000
Hydrogen production by biological processes: a survey of literature Das, D. International Journal of Hydrogen Energy, Vol. 26, Issue 1 https://doi.org/10.1016/S0360-3199(00)00058-6	journal	January 2001
Biological hydrogen production; fundamentals and limiting processes Hallenbeck, P. International Journal of Hydrogen Energy, Vol. 27, Issue 11-12 https://doi.org/10.1016/S0360-3199(02)00131-3	journal	November 2002
Taking toll: lipid A mimetics as adjuvants and immunomodulators Persing, David H.; Coler, Rhea N.; Lacy, Michael J. Trends in Microbiology, Vol. 10, Issue 10, p. s32-s37 https://doi.org/10.1016/S0966-842X(02)02426-5	journal	October 2002
Structure and function of cytochromes P450:a comparative analysis of three crystal structures Hasemann, Charles A.; Kurumbail, Ravi G.; Boddupalli, Sekhar S. Structure, Vol. 3, Issue 1, p. 41-62 https://doi.org/10.1016/S0969-2126(01)00134-4	journal	January 1995
The growing impact of click chemistry on drug discovery Kolb, Hartmuth C.; Sharpless, K. Barry Drug Discovery Today, Vol. 8, Issue 24, p. 1128-1137 https://doi.org/10.1016/S1359-6446(03)02933-7	journal	December 2003
Induction of tumor necrosis factor-α in solid tumor region by the orally administered synthetic muramyl dipeptide analogue, romurtide Ueda, Hiroshi; Yamazaki, Masatoshi International Immunopharmacology, Vol. 1, Issue 1, p. 97-104 https://doi.org/10.1016/S1567-5769(00)00017-5	journal	January 2001
Co-incorporation of heterologously expressed Arabidopsis cytochrome P450 and P450 reductase into soluble nanoscale lipid bilayers Duan, Hui; Civjan, Natanya R; Sligar, Stephen G Archives of Biochemistry and Biophysics, Vol. 424, Issue 2, p. 141-153 https://doi.org/10.1016/j.abb.2004.02.010	journal	April 2004
Homotropic cooperativity of monomeric cytochrome P450 3A4 in a nanoscale native bilayer environment Baas, Bradley J.; Denisov, Ilia G.; Sligar, Stephen G. Archives of Biochemistry and Biophysics, Vol. 430, Issue 2, p. 218-228 https://doi.org/10.1016/j.abb.2004.07.003	journal	October 2004
Indirect electrochemical reduction of nicotinamide coenzymes Vuorilehto, K.; Lütz, S.; Wandrey, C. Bioelectrochemistry, Vol. 65, Issue 1, p. 1-7 https://doi.org/10.1016/j.bioelechem.2004.05.006	journal	December 2004
Bio-hydrogen production from waste materials Kapdan, Ilgi Karapinar; Kargi, Fikret Enzyme and Microbial Technology, Vol. 38, Issue 5, p. 569-582 https://doi.org/10.1016/j.enzmictec.2005.09.015	journal	March 2006
Mechanical properties and stability measurement of cholesterol-containing liposome on mica by atomic force microscopy Liang, Xuemei; Mao, Guangzhao; Ng, K. Y. Simon Journal of Colloid and Interface Science, Vol. 278, Issue 1, p. 53-62 https://doi.org/10.1016/j.jcis.2004.05.042	journal	October 2004
Stable isotope probing: Technical considerations when resolving ¹⁵N-labeled RNA in gradients Addison, S. L.; McDonald, I. R.; Lloyd-Jones, G. Journal of Microbiological Methods, Vol. 80, Issue 1, p. 70-75 https://doi.org/10.1016/j.mimet.2009.11.002	journal	January 2010
Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors Ishihara, Goshi; Goto, Mie; Saeki, Mihoro Protein Expression and Purification, Vol. 41, Issue 1, p. 27-37 https://doi.org/10.1016/j.pep.2005.01.013	journal	May 2005
Initiation of rubber biosynthesis: In vitro comparisons of benzophenone-modified diphosphate analogues in three rubber-producing species Xie, Wenshuang; McMahan, Colleen M.; DeGraw, Amanda J. Phytochemistry, Vol. 69, Issue 14, p. 2539-2545 https://doi.org/10.1016/j.phytochem.2008.07.011	journal	October 2008
Transcriptome and gene expression analysis in cold-acclimated guayule (Parthenium argentatum) rubber-producing tissue Ponciano, Grisel; McMahan, Colleen M.; Xie, Wenshuang Phytochemistry, Vol. 79, p. 57-66 https://doi.org/10.1016/j.phytochem.2012.04.007	journal	July 2012
DNA-based stable isotope probing: a link between community structure and function Uhlík, Ondrej; Jecná, Katerina; Leigh, Mary Beth Science of The Total Environment, Vol. 407, Issue 12, p. 3611-3619 https://doi.org/10.1016/j.scitotenv.2008.05.012	journal	June 2009
Detection of DNA hybridization on indium tin oxide surfaces Moses, Selina; Brewer, Scott H.; Kraemer, Stephan Sensors and Actuators B: Chemical, Vol. 125, Issue 2, p. 574-580 https://doi.org/10.1016/j.snb.2007.03.009	journal	August 2007
Construction and characterization of a second-generation pseudoinfectious West Nile virus vaccine propagated using a new cultivation system Widman, Douglas G.; Ishikawa, Tomohiro; Fayzulin, Rafik Vaccine, Vol. 26, Issue 22, p. 2762-2771 https://doi.org/10.1016/j.vaccine.2008.03.009	journal	May 2008
High-Sensitivity Detection of DNA Hybridization on Microarrays Using Resonance Light Scattering Bao, Paul; Frutos, Anthony G.; Greef, Charles Analytical Chemistry, Vol. 74, Issue 8 https://doi.org/10.1021/ac0111964	journal	April 2002
Gold Nanoparticle-Enhanced Secondary Ion Mass Spectrometry Imaging of Peptides on Self-Assembled Monolayers Kim, Young-Pil; Oh, Eunkeu; Hong, Mi-Young Analytical Chemistry, Vol. 78, Issue 6 https://doi.org/10.1021/ac051500j	journal	March 2006
Conjugation to Nickel-Chelating Nanolipoprotein Particles Increases the Potency and Efficacy of Subunit Vaccines to Prevent West Nile Encephalitis Fischer, Nicholas O.; Infante, Ernesto; Ishikawa, Tomohiro Bioconjugate Chemistry, Vol. 21, Issue 6, p. 1018-1022 https://doi.org/10.1021/bc100083d	journal	June 2010
Immobilization of His-Tagged Proteins on Nickel-Chelating Nanolipoprotein Particles Fischer, Nicholas O.; Blanchette, Craig D.; Chromy, Brett A. Bioconjugate Chemistry, Vol. 20, Issue 3 https://doi.org/10.1021/bc8003155	journal	March 2009
Indium-111 Labeling of Low Density Lipoproteins with the DTPA−Bis(stearylamide): Evaluation as a Potential Radiopharmaceutical for Tumor Localization Jasanada, François; Urizzi, Pascale; Souchard, Jean-Pierre Bioconjugate Chemistry, Vol. 7, Issue 1 https://doi.org/10.1021/bc950073l	journal	January 1996
Synthesis and Characterization of Chelator-Lipids for Reversible Immobilization of Engineered Proteins at Self-Assembled Lipid Interfaces Schmitt, Lutz; Dietrich, Christian; Tampe, Robert Journal of the American Chemical Society, Vol. 116, Issue 19, p. 8485-8491 https://doi.org/10.1021/ja00098a008	journal	September 1994
Systems for orthogonal self-assembly of electroactive monolayers on Au and ITO: an approach to molecular electronics Gardner, Timothy J.; Frisbie, C. Daniel; Wrighton, Mark S. Journal of the American Chemical Society, Vol. 117, Issue 26, p. 6927-6933 https://doi.org/10.1021/ja00131a015	journal	July 1995
The [NiFe]-Hydrogenase of the Cyanobacterium Synechocystis sp. PCC 6803 Works Bidirectionally with a Bias to H ₂ Production McIntosh, Chelsea L.; Germer, Frauke; Schulz, Rüdiger Journal of the American Chemical Society, Vol. 133, Issue 29 https://doi.org/10.1021/ja203376y	journal	July 2011
Hydrogen Production by a Hyperthermophilic Membrane-Bound Hydrogenase in Water-Soluble Nanolipoprotein Particles Baker, Sarah E.; Hopkins, Robert C.; Blanchette, Craig D. Journal of the American Chemical Society, Vol. 131, Issue 22 https://doi.org/10.1021/ja809251f	journal	June 2009
The Efficiency of Light-Directed Synthesis of DNA Arrays on Glass Substrates McGall, Glenn H.; Barone, Anthony D.; Diggelmann, Martin Journal of the American Chemical Society, Vol. 119, Issue 22, p. 5081-5090 https://doi.org/10.1021/ja964427a	journal	June 1997
Formation of Thiolate and Phosphonate Adlayers on Indium−Tin Oxide: Optical and Electronic Characterization Brewer, Scott H.; Brown, Derek A.; Franzen, Stefan Langmuir, Vol. 18, Issue 18 https://doi.org/10.1021/la015720d	journal	September 2002
Two-Dimensional Streptavidin Crystals on Giant Lipid Bilayer Vesicles Ratanabanangkoon, Pasut; Gropper, Michael; Merkel, Rudolf Langmuir, Vol. 18, Issue 11, p. 4270-4276 https://doi.org/10.1021/la025568v	journal	May 2002
Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach Katzen, Federico; Fletcher, Julia E.; Yang, Jian-Ping Journal of Proteome Research, Vol. 7, Issue 8 https://doi.org/10.1021/pr800265f	journal	August 2008
Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array Singh-Gasson, Sangeet; Green, Roland D.; Yue, Yongjian Nature Biotechnology, Vol. 17, Issue 10, p. 974-978 https://doi.org/10.1038/13664	journal	October 1999
Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers Boschker, H. T. S.; Nold, S. C.; Wellsbury, P. Nature, Vol. 392, Issue 6678 https://doi.org/10.1038/33900	journal	April 1998
Stable-isotope probing as a tool in microbial ecology Radajewski, Stefan; Ineson, Philip; Parekh, Nisha R. Nature, Vol. 403, Issue 6770, p. 646-649 https://doi.org/10.1038/35001054	journal	February 2000
Exploring the new world of the genome with DNA microarrays Brown, Patrick O.; Botstein, David Nature Genetics, Vol. 21, Issue S1 https://doi.org/10.1038/4462	journal	January 1999
Vaccine manufacturing: challenges and solutions Ulmer, Jeffrey B.; Valley, Ulrich; Rappuoli, Rino Nature Biotechnology, Vol. 24, Issue 11 https://doi.org/10.1038/nbt1261	journal	November 2006
Large-scale profiling of protein palmitoylation in mammalian cells Martin, Brent R.; Cravatt, Benjamin F. Nature Methods, Vol. 6, Issue 2, p. 135-138 https://doi.org/10.1038/nmeth.1293	journal	January 2009
Integration of biological networks and gene expression data using Cytoscape Cline, Melissa S.; Smoot, Michael; Cerami, Ethan Nature Protocols, Vol. 2, Issue 10 https://doi.org/10.1038/nprot.2007.324	journal	September 2007
Endothelin-1 enhances oxidative stress, cell proliferation and reduces apoptosis in human umbilical vein endothelial cells: role of ET _B receptor, NADPH oxidase and caveolin-1 Dong, Feng; Zhang, Xiaochun; Wold, Loren E. British Journal of Pharmacology, Vol. 145, Issue 3 https://doi.org/10.1038/sj.bjp.0706193	journal	June 2005
Three-Dimensional Graphene Nano-Networks with High Quality and Mass Production Capability via Precursor-Assisted Chemical Vapor Deposition Yoon, Jong-Chul; Lee, Jung-Soo; Kim, Sun-I Scientific Reports, Vol. 3, Issue 1 https://doi.org/10.1038/srep01788	journal	May 2013
Steric stabilization of phospholipid vesicles by block copolymers Vesicle flocculation and osmotic swelling caused by monovalent and divalent cations Kostarelos, Kostas; Luckham, Paul F.; Tadros, Tharwat F. Journal of the Chemical Society, Faraday Transactions, Vol. 94, Issue 15 https://doi.org/10.1039/A801134F	journal	January 1998
Biochemistry of natural rubber, a vital raw material, emphasizing biosynthetic rate, molecular weight and compartmentalization, in evolutionarily divergent plant species (1963 to 2000) Cornish, Katrina Natural Product Reports, Vol. 18, Issue 2 https://doi.org/10.1039/A902191D	journal	January 2001
Progress towards the easier use of P450 enzymes Chefson, Amandine; Auclair, Karine Molecular BioSystems, Vol. 2, Issue 10 https://doi.org/10.1039/B607001A	journal	January 2006
An improved synthesis of isopentenyl pyrophosphate Donninger, C.; Popják, G. Biochemical Journal, Vol. 105, Issue 2 https://doi.org/10.1042/bj1050545	journal	November 1967
Amino acids in SRS1 and SRS6 are critical for furanocoumarin metabolism by CYP6B1v1, a cytochrome P450 monooxygenase Chen, J.-S.; Berenbaum, M. R.; Schuler, M. A. Insect Molecular Biology, Vol. 11, Issue 2, p. 175-186 https://doi.org/10.1046/j.1365-2583.2002.00323.x	journal	April 2002
Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans: CO-oxidizing:H2-evolving enzyme complex Soboh, Basem; Linder, Dietmar; Hedderich, Reiner European Journal of Biochemistry, Vol. 269, Issue 22 https://doi.org/10.1046/j.1432-1033.2002.03282.x	journal	October 2002
The membrane-bound [NiFe]-hydrogenase (Ech) from Methanosarcina barkeri: unusual properties of the iron-sulphur clusters: The membrane-bound [NiFe]-hydrogenase (Ech) from M. barkeri Kurkin, Sergei; Meuer, Jörn; Koch, Jürgen European Journal of Biochemistry, Vol. 269, Issue 24 https://doi.org/10.1046/j.1432-1033.2002.03328.x	journal	December 2002
Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri Meuer, Jorn; Bartoschek, Stefan; Koch, Jurgen European Journal of Biochemistry, Vol. 265, Issue 1 https://doi.org/10.1046/j.1432-1327.1999.00738.x	journal	October 1999
Protein tolerance to random amino acid change Guo, H. H.; Choe, J.; Loeb, L. A. Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210 https://doi.org/10.1073/pnas.0403255101	journal	June 2004
Different G protein-coupled receptor kinases govern G protein and β-arrestin-mediated signaling of V2 vasopressin receptor Ren, X.-R.; Reiter, E.; Ahn, S. Proceedings of the National Academy of Sciences, Vol. 102, Issue 5, p. 1448-1453 https://doi.org/10.1073/pnas.0409534102	journal	January 2005
Urban aerosols harbor diverse and dynamic bacterial populations Brodie, E. L.; DeSantis, T. Z.; Parker, J. P. M. Proceedings of the National Academy of Sciences, Vol. 104, Issue 1, p. 299-304 https://doi.org/10.1073/pnas.0608255104	journal	December 2006
A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein Whorton, M. R.; Bokoch, M. P.; Rasmussen, S. G. F. Proceedings of the National Academy of Sciences, Vol. 104, Issue 18, p. 7682-7687 https://doi.org/10.1073/pnas.0611448104	journal	April 2007
Single-molecule height measurements on microsomal cytochrome P450 in nanometer-scale phospholipid bilayer disks Bayburt, T. H.; Sligar, S. G. Proceedings of the National Academy of Sciences, Vol. 99, Issue 10, p. 6725-6730 https://doi.org/10.1073/pnas.062565599	journal	May 2002
Dopamine tone regulates D1 receptor trafficking and delivery in striatal neurons in dopamine transporter-deficient mice Dumartin, B.; Jaber, M.; Gonon, F. Proceedings of the National Academy of Sciences, Vol. 97, Issue 4, p. 1879-1884 https://doi.org/10.1073/pnas.97.4.1879	journal	February 2000
The Major Protein of Guayule Rubber Particles Is a Cytochrome P450: CHARACTERIZATION BASED ON cDNA CLONING AND SPECTROSCOPIC ANALYSIS OF THE SOLUBILIZED ENZYME AND ITS REACTION PRODUCTS Pan, Zhiqiang; Durst, Francis; Werck-Reichhart, Daniele Journal of Biological Chemistry, Vol. 270, Issue 15 https://doi.org/10.1074/jbc.270.15.8487	journal	April 1995
Intact Functional Fourteen-subunit Respiratory Membrane-bound [NiFe]-Hydrogenase Complex of the Hyperthermophilic Archaeon Pyrococcus furiosus McTernan, Patrick M.; Chandrayan, Sanjeev K.; Wu, Chang-Hao Journal of Biological Chemistry, Vol. 289, Issue 28 https://doi.org/10.1074/jbc.M114.567255	journal	May 2014
Model of Biologically Active Apolipoprotein E Bound to Dipalmitoylphosphatidylcholine Peters-Libeu, Clare A.; Newhouse, Yvonne; Hatters, Danny M. Journal of Biological Chemistry, Vol. 281, Issue 2 https://doi.org/10.1074/jbc.M510851200	journal	November 2005
Transducin Activation by Nanoscale Lipid Bilayers Containing One and Two Rhodopsins Bayburt, Timothy H.; Leitz, Andrew J.; Xie, Guifu Journal of Biological Chemistry, Vol. 282, Issue 20 https://doi.org/10.1074/jbc.M701433200	journal	March 2007
Cell-free Co-expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles Cappuccio, Jenny A.; Blanchette, Craig D.; Sulchek, Todd A. Molecular & Cellular Proteomics, Vol. 7, Issue 11 https://doi.org/10.1074/mcp.M800191-MCP200	journal	July 2008
Generation of immunoliposomes using recombinant single-chain Fv fragments bound to Ni-NTA-liposomes Rüger, Ronny; Müller, Dafne; Fahr, Alfred Journal of Drug Targeting, Vol. 13, Issue 7 https://doi.org/10.1080/10611860500353328	journal	August 2005
In vitro characterization of binding and stability of single-chain Fv Ni-NTA-liposomes Rüger, Ronny; Müller, Dafne; Fahr, Alfred Journal of Drug Targeting, Vol. 14, Issue 8 https://doi.org/10.1080/10611860600864018	journal	January 2006
The micromorphology and protein characterization of rubber particles in Ficus carica, Ficus benghalensis and Hevea brasiliensis Singh, A. P.; Wi, S. G.; Chung, G. C. Journal of Experimental Botany, Vol. 54, Issue 384 https://doi.org/10.1093/jxb/erg107	journal	March 2003
Protein engineering methods applied to membrane protein targets Lluis, M. W.; Godfroy, J. I.; Yin, H. Protein Engineering Design and Selection, Vol. 26, Issue 2 https://doi.org/10.1093/protein/gzs079	journal	October 2012
Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles Sperling, R. A.; Parak, W. J. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 368, Issue 1915, p. 1333-1383 https://doi.org/10.1098/rsta.2009.0273	journal	February 2010
Identification of active methylotroph populations in an acidic forest soil by stable-isotope probing c Radajewski, Stefan; Prosser, James I.; Nedwell, David B. Microbiology, Vol. 148, Issue 8 https://doi.org/10.1099/00221287-148-8-2331	journal	August 2002
A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis Soboh, B. Microbiology, Vol. 150, Issue 7 https://doi.org/10.1099/mic.0.27159-0	journal	July 2004
Modulation of human erg K⁺ channel gating by activation of a G protein-coupled receptor and protein kinase C Barros, Francisco; Gómez-Varela, David; Viloria, Cristina G. The Journal of Physiology, Vol. 511, Issue 2, p. 333-346 https://doi.org/10.1111/j.1469-7793.1998.333bh.x	journal	September 1998
The Effects of Deleting the Mouse Neurotensin Receptor NTR1 on Central and Peripheral Responses to Neurotensin Pettibone, Douglas J.; Hess, J. Fred; Hey, Patricia J. Journal of Pharmacology and Experimental Therapeutics, Vol. 300, Issue 1 https://doi.org/10.1124/jpet.300.1.305	journal	January 2002
The Vaccine Adjuvant Monophosphoryl Lipid A as a TRIF-Biased Agonist of TLR4 Mata-Haro, V.; Cekic, C.; Martin, M. Science, Vol. 316, Issue 5831, p. 1628-1632 https://doi.org/10.1126/science.1138963	journal	June 2007
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB DeSantis, T. Z.; Hugenholtz, P.; Larsen, N. Applied and Environmental Microbiology, Vol. 72, Issue 7, p. 5069-5072 https://doi.org/10.1128/AEM.03006-05	journal	July 2006
RNA Stable Isotope Probing, a Novel Means of Linking Microbial Community Function to Phylogeny Manefield, M.; Whiteley, A. S.; Griffiths, R. I. Applied and Environmental Microbiology, Vol. 68, Issue 11 https://doi.org/10.1128/AEM.68.11.5367-5373.2002	journal	November 2002
The Isotope Array, a New Tool That Employs Substrate-Mediated Labeling of rRNA for Determination of Microbial Community Structure and Function Adamczyk, J.; Hesselsoe, M.; Iversen, N. Applied and Environmental Microbiology, Vol. 69, Issue 11 https://doi.org/10.1128/AEM.69.11.6875-6887.2003	journal	November 2003
Cyanobacterial-Type, Heteropentameric, NAD+-Reducing NiFe Hydrogenase in the Purple Sulfur Photosynthetic Bacterium Thiocapsa roseopersicina Rakhely, G.; Kovacs, A. T.; Maroti, G. Applied and Environmental Microbiology, Vol. 70, Issue 2 https://doi.org/10.1128/AEM.70.2.722-728.2004	journal	February 2004
Biodegradation of Natural Rubber and Related Compounds: Recent Insights into a Hardly Understood Catabolic Capability of Microorganisms Rose, K.; Steinbuchel, A. Applied and Environmental Microbiology, Vol. 71, Issue 6 https://doi.org/10.1128/AEM.71.6.2803-2812.2005	journal	June 2005
The Soluble NAD⁺-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 Consists of Six Subunits and Can Be Specifically Activated by NADPH Burgdorf, T.; van der Linden, E.; Bernhard, M. Journal of Bacteriology, Vol. 187, Issue 9, p. 3122-3132 https://doi.org/10.1128/JB.187.9.3122-3132.2005	journal	April 2005
Noncytopathic Replication of Venezuelan Equine Encephalitis Virus and Eastern Equine Encephalitis Virus Replicons in Mammalian Cells Petrakova, O.; Volkova, E.; Gorchakov, R. Journal of Virology, Vol. 79, Issue 12 https://doi.org/10.1128/JVI.79.12.7597-7608.2005	journal	May 2005
Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities. Lazar, E.; Watanabe, S.; Dalton, S. Molecular and Cellular Biology, Vol. 8, Issue 3, p. 1247-1252 https://doi.org/10.1128/MCB.8.3.1247	journal	March 1988
Synthesis of Native Proteins by Chemical Ligation Dawson, Philip E.; Kent, Stephen B. H. Annual Review of Biochemistry, Vol. 69, Issue 1 https://doi.org/10.1146/annurev.biochem.69.1.923	journal	June 2000
How Translocons Select Transmembrane Helices White, Stephen H.; von Heijne, Gunnar Annual Review of Biophysics, Vol. 37, Issue 1 https://doi.org/10.1146/annurev.biophys.37.032807.125904	journal	June 2008
G-Protein-Coupled Receptor Microarrays for Multiplexed Compound Screening Hong, Yulong; Webb, Brian L.; Pai, Sadashiva Journal of Biomolecular Screening, Vol. 11, Issue 4 https://doi.org/10.1177/1087057106287139	journal	June 2006
Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz Schmidt, Thomas; Lenders, Malte; Hillebrand, Andrea BMC Biochemistry, Vol. 11, Issue 1 https://doi.org/10.1186/1471-2091-11-11	journal	January 2010
High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry Lechene, Claude; Hillion, Francois; McMahon, Greg Journal of Biology, Vol. 5, Issue 6 https://doi.org/10.1186/jbiol42	journal	October 2006
Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties Berthelot, Karine; Lecomte, Sophie; Estevez, Yannick PLoS ONE, Vol. 7, Issue 10 https://doi.org/10.1371/journal.pone.0048065	journal	October 2012
Evaluation of Nanolipoprotein Particles (NLPs) as an In Vivo Delivery Platform Fischer, Nicholas O.; Weilhammer, Dina R.; Dunkle, Alexis PLoS ONE, Vol. 9, Issue 3 https://doi.org/10.1371/journal.pone.0093342	journal	March 2014
Molecular Dynamics Simulations of Discoidal Bilayers Assembled from Truncated Human Lipoproteins Shih, Amy Y.; Denisov, Ilia G.; Phillips, James C. Biophysical Journal, Vol. 88, Issue 1 https://doi.org/10.1529/biophysj.104.046896	journal	January 2005
Enzyme-catalyzed Synthesis and Degradation of Biopolymers Hiraishi, Tomohiro; Taguchi, Seiichi Mini-Reviews in Organic Chemistry, Vol. 6, Issue 1 https://doi.org/10.2174/157019309787316139	journal	February 2009
Corticotropin Releasing Factor (CRF) Receptor Signaling in the Central Nervous System: New Molecular Targets Hauger, Richard; Risbrough, Victoria; Brauns, Olaf CNS & Neurological Disorders - Drug Targets, Vol. 5, Issue 4 https://doi.org/10.2174/187152706777950684	journal	August 2006
Some Observations on the Type I and Type II Microsomal Binding Spectra Gorrod, J. W.; Disley, L. G.; Temple, D. J. Xenobiotica, Vol. 1, Issue 4-5 https://doi.org/10.3109/00498257109041520	journal	January 1971
Characterization and Purification of Polydisperse Reconstituted Lipoproteins and Nanolipoprotein Particles Blanchette, Craig; Segelke, Brent; Fischer, Nicholas International Journal of Molecular Sciences, Vol. 10, Issue 7 https://doi.org/10.3390/ijms10072958	journal	July 2009
The contribution of macrophyte-derived organic matter to microbial biomass in salt-marsh sediments: Stable carbon isotope analysis of microbial biomarkers Boschker, H. T. S.; de Brouwer, J. F. C.; Cappenberg, T. E. Limnology and Oceanography, Vol. 44, Issue 2 https://doi.org/10.4319/lo.1999.44.2.0309	journal	March 1999

Similar Records

Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Patent · Tue Oct 04 00:00:00 EDT 2016 · OSTI ID:1327928

Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Patent · Mon Dec 08 23:00:00 EST 2014 · OSTI ID:1165126

Characterization and Purification of Polydisperse Reconstituted Lipoproteins and Nanolipoprotein Particles

Journal Article · Tue Jun 30 20:00:00 EDT 2009 · International Journal of Molecular Sciences (Online) · OSTI ID:1628357

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES

Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Citation Formats

References (121)

Similar Records

Related Subjects