Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Chromy, Brett A; Henderson, Paul; Hoeprich, Jr., Paul D

Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Patent · Tue Dec 09 04:00:00 EST 2014

OSTI ID:1165126

Chromy, Brett A; Henderson, Paul; Hoeprich, Jr., Paul D

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC52-07NA27344

Assignee:: Lawrence Livermore National Security, LLC. (Livermore, CA)

Patent Number(s):: 8,907,061

Application Number:: 12/352,548

OSTI ID:: 1165126

Country of Publication:: United States

Language:: English

References (54)

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