Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Patent · Tue Oct 04 04:00:00 EDT 2016

OSTI ID:1327928

Chromy, Brett A.; Henderson, Paul; Hoeprich, Jr, Paul D.

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC52-07NA27344
• Assignee: Lawrence Livermore National Security, LLC (Livermore, CA)
• Patent Number(s): 9,458,191
• Application Number: 14/536,513
• OSTI ID: 1327928
• Country of Publication: United States
• Language: English