Lignin conversion: Opportunities and challenges for the integrated biorefinery

Xie, Shangxian; Ragauskas, Arthur J.; Yuan, Joshua S.

doi:10.1089/ind.2016.0007

Lignin conversion: Opportunities and challenges for the integrated biorefinery

Journal Article · Tue Jun 21 00:00:00 EDT 2016 · Industrial Biotechnology

DOI:https://doi.org/10.1089/ind.2016.0007· OSTI ID:1327723

Xie, Shangxian ^[1]; Ragauskas, Arthur J. ^[2]; Yuan, Joshua S. ^[1]

Texas A & M Univ., College Station, TX (United States)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

The utilization of lignin for fungible fuels and products represents one of the most imminent challenges in the modern biorefinery because most of the bioprocesses for lignocellulosic biofuels results in a lignin-containing waste stream. Considering lignin's abundance and relatively high energy content, this waste stream can be used as a feedstock for value-added products to improve the sustainability and economic feasibility of the biorefinery. Bioconversion of lignin with microbes recently emerged as an alternative lignin-valorization approach with significant potential. Typically, the microbial bioconversion of lignin requires three major steps: lignin depolymerization, aromatic compounds catabolism, and target product biosynthesis. In this review, we summarize the most recent advances in lignin bioconversion to address the challenges in each of the three steps. In conclusion, we further discuss strategies and perspectives for future research to address the challenges in bioconversion of lignin.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1327723

Alternate ID(s):: OSTI ID: 1376578

Journal Information:: Industrial Biotechnology, Journal Name: Industrial Biotechnology Journal Issue: 3 Vol. 12; ISSN 1550-9087

Publisher:: Mary Ann Liebert, Inc.Copyright Statement

Country of Publication:: United States

Language:: English

References (47)

Pretreatment: the key to unlocking low-cost cellulosic ethanol Yang, Bin; Wyman, Charles E. Biofuels, Bioproducts and Biorefining, Vol. 2, Issue 1 https://doi.org/10.1002/bbb.49	journal	January 2008
Lignin Depolymerization and Conversion A Review of Thermochemical Methods Pandey, M. P.; Kim, C. S. Chemical Engineering & Technology, Vol. 34, Issue 1, p. 29-41 https://doi.org/10.1002/ceat.201000270	journal	November 2010
Efficient production of 2-pyrone 4,6-dicarboxylic acid as a novel polymer-based material from protocatechuate by microbial function Otsuka, Yuichiro; Nakamura, Masaya; Shigehara, Kiyotaka Applied Microbiology and Biotechnology, Vol. 71, Issue 5 https://doi.org/10.1007/s00253-005-0203-7	journal	December 2005
Bioconversion of lignin model compounds with oleaginous Rhodococci Kosa, Matyas; Ragauskas, Arthur J. Applied Microbiology and Biotechnology, Vol. 93, Issue 2 https://doi.org/10.1007/s00253-011-3743-z	journal	December 2011
Catalytic hydrotreatment of some technical lignins Oasmaa, Anja; Alén, Raimo; Meier, Dietrich Bioresource Technology, Vol. 45, Issue 3 https://doi.org/10.1016/0960-8524(93)90111-N	journal	January 1993
Can laccases catalyze bond cleavage in lignin? Munk, Line; Sitarz, Anna K.; Kalyani, Dayanand C. Biotechnology Advances, Vol. 33, Issue 1 https://doi.org/10.1016/j.biotechadv.2014.12.008	journal	January 2015
The emerging role for bacteria in lignin degradation and bio-product formation Bugg, Timothy DH; Ahmad, Mark; Hardiman, Elizabeth M. Current Opinion in Biotechnology, Vol. 22, Issue 3 https://doi.org/10.1016/j.copbio.2010.10.009	journal	June 2011
Exploration of Natural Biomass Utilization Systems (NBUS) for advanced biofuel—from systems biology to synthetic design Xie, Shangxian; Syrenne, Ryan; Sun, Su Current Opinion in Biotechnology, Vol. 27 https://doi.org/10.1016/j.copbio.2014.02.007	journal	June 2014
Review of current and future softwood kraft lignin process chemistry Chakar, Fadi S.; Ragauskas, Arthur J. Industrial Crops and Products, Vol. 20, Issue 2 https://doi.org/10.1016/j.indcrop.2004.04.016	journal	September 2004
Biochemical and molecular characterization of a novel laccase from selective lignin-degrading white-rot fungus Echinodontium taxodii 2538 Shi, Lili; Yu, Hongbo; Dong, Tianbao Process Biochemistry, Vol. 49, Issue 7 https://doi.org/10.1016/j.procbio.2014.03.028	journal	July 2014
What is (and is not) vital to advancing cellulosic ethanol Wyman, Charles E. Trends in Biotechnology, Vol. 25, Issue 4 https://doi.org/10.1016/j.tibtech.2007.02.009	journal	April 2007
Plant systems biology comes of age Yuan, Joshua S.; Galbraith, David W.; Dai, Susie Y. Trends in Plant Science, Vol. 13, Issue 4 https://doi.org/10.1016/j.tplants.2008.02.003	journal	April 2008
Plants to power: bioenergy to fuel the future Yuan, Joshua S.; Tiller, Kelly H.; Al-Ahmad, Hani Trends in Plant Science, Vol. 13, Issue 8 https://doi.org/10.1016/j.tplants.2008.06.001	journal	August 2008
Identification of DypB from Rhodococcus jostii RHA1 as a Lignin Peroxidase Ahmad, Mark; Roberts, Joseph N.; Hardiman, Elizabeth M. Biochemistry, Vol. 50, Issue 23 https://doi.org/10.1021/bi101892z	journal	June 2011
Breaking Down Lignin to High-Value Chemicals: The Conversion of Lignocellulose to Vanillin in a Gene Deletion Mutant of Rhodococcus jostii RHA1 Sainsbury, Paul D.; Hardiman, Elizabeth M.; Ahmad, Mark ACS Chemical Biology, Vol. 8, Issue 10 https://doi.org/10.1021/cb400505a	journal	August 2013
Heteronuclear Single-Quantum Correlation–Nuclear Magnetic Resonance (HSQC–NMR) Fingerprint Analysis of Pyrolysis Oils Ben, Haoxi; Ragauskas, Arthur J. Energy & Fuels, Vol. 25, Issue 12 https://doi.org/10.1021/ef201376w	journal	December 2011
Loosening lignin's grip on biofuel production Chapple, Clint; Ladisch, Michael; Meilan, Rick Nature Biotechnology, Vol. 25, Issue 7, p. 746-748 https://doi.org/10.1038/nbt0707-746	journal	July 2007
Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78 Martinez, Diego; Larrondo, Luis F.; Putnam, Nik Nature Biotechnology, Vol. 22, Issue 6 https://doi.org/10.1038/nbt967	journal	May 2004
Microbial degradation of aromatic compounds — from one strategy to four Fuchs, Georg; Boll, Matthias; Heider, Johann Nature Reviews Microbiology, Vol. 9, Issue 11, p. 803-816 https://doi.org/10.1038/nrmicro2652	journal	October 2011
Adipic acid production from lignin Vardon, Derek R.; Franden, Mary Ann; Johnson, Christopher W. Energy & Environmental Science, Vol. 8, Issue 2 https://doi.org/10.1039/C4EE03230F	journal	January 2015
Simultaneous conversion of all cell wall components by an oleaginous fungus without chemi-physical pretreatment Xie, Shangxian; Qin, Xing; Cheng, Yanbing Green Chemistry, Vol. 17, Issue 3 https://doi.org/10.1039/C4GC01529K	journal	January 2015
Bioconversion of oxygen-pretreated Kraft lignin to microbial lipid with oleaginous Rhodococcus opacus DSM 1069 Wei, Zhen; Zeng, Guangming; Huang, Fang Green Chemistry, Vol. 17, Issue 5 https://doi.org/10.1039/C5GC00422E	journal	January 2015
Synergistic enzymatic and microbial lignin conversion Zhao, Cheng; Xie, Shangxian; Pu, Yunqiao Green Chemistry, Vol. 18, Issue 5 https://doi.org/10.1039/C5GC01955A	journal	January 2016
Lignin to lipid bioconversion by oleaginous Rhodococci Kosa, Matyas; Ragauskas, Arthur J. Green Chemistry, Vol. 15, Issue 8 https://doi.org/10.1039/c3gc40434j	journal	January 2013
Net energy of cellulosic ethanol from switchgrass Schmer, M. R.; Vogel, K. P.; Mitchell, R. B. Proceedings of the National Academy of Sciences, Vol. 105, Issue 2 https://doi.org/10.1073/pnas.0704767105	journal	January 2008
Lignin degradation in wood-feeding insects Geib, S. M.; Filley, T. R.; Hatcher, P. G. Proceedings of the National Academy of Sciences, Vol. 105, Issue 35 https://doi.org/10.1073/pnas.0805257105	journal	August 2008
Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis Fernandez-Fueyo, E.; Ruiz-Duenas, F. J.; Ferreira, P. Proceedings of the National Academy of Sciences, Vol. 109, Issue 14, p. 5458-5463 https://doi.org/10.1073/pnas.1119912109	journal	March 2012
Lignin valorization through integrated biological funneling and chemical catalysis Linger, J. G.; Vardon, D. R.; Guarnieri, M. T. Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018 https://doi.org/10.1073/pnas.1410657111	journal	August 2014
Comparative transcriptomics elucidates adaptive phenol tolerance and utilization in lipid-accumulating Rhodococcus opacus PD630 Yoneda, Aki; Henson, William R.; Goldner, Nicholas K. Nucleic Acids Research, Vol. 44, Issue 5 https://doi.org/10.1093/nar/gkw055	journal	February 2016
The Path Forward for Biofuels and Biomaterials Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H. Science, Vol. 311, Issue 5760, p. 484-489 https://doi.org/10.1126/science.1114736	journal	January 2006
The Plant Cell Wall-Decomposing Machinery Underlies the Functional Diversity of Forest Fungi Eastwood, D. C.; Floudas, D.; Binder, M. Science, Vol. 333, Issue 6043, p. 762-765 https://doi.org/10.1126/science.1205411	journal	July 2011
The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes Floudas, Dimitrios; Binder, Manfred; Riley, Robert Science, Vol. 336, Issue 6089 https://doi.org/10.1126/science.1221748	journal	June 2012
Transcriptomic Assessment of Isozymes in the Biphenyl Pathway of Rhodococcus sp. Strain RHA1 Goncalves, E. R.; Hara, H.; Miyazawa, D. Applied and Environmental Microbiology, Vol. 72, Issue 9 https://doi.org/10.1128/AEM.00947-06	journal	September 2006
Involvement of a Novel ABC Transporter and Monoalkyl Phthalate Ester Hydrolase in Phthalate Ester Catabolism by Rhodococcus jostii RHA1 Hara, Hirofumi; Stewart, Gordon R.; Mohn, William W. Applied and Environmental Microbiology, Vol. 76, Issue 5 https://doi.org/10.1128/AEM.02621-09	journal	March 2010
Vanillin Catabolism in Rhodococcus jostii RHA1 Chen, Hao-Ping; Chow, Mindy; Liu, Chi-Chun Applied and Environmental Microbiology, Vol. 78, Issue 2 https://doi.org/10.1128/AEM.06876-11	journal	November 2011
Roles of Ring-Hydroxylating Dioxygenases in Styrene and Benzene Catabolism in Rhodococcus jostii RHA1 Patrauchan, M. A.; Florizone, C.; Eapen, S. Journal of Bacteriology, Vol. 190, Issue 1 https://doi.org/10.1128/JB.01122-07	journal	October 2007
Transcriptomic Analysis Reveals a Bifurcated Terephthalate Degradation Pathway in Rhodococcus sp. Strain RHA1 Hara, Hirofumi; Eltis, Lindsay D.; Davies, Julian E. Journal of Bacteriology, Vol. 189, Issue 5 https://doi.org/10.1128/JB.01322-06	journal	December 2006
Catabolism of Benzoate and Phthalate in Rhodococcus sp. Strain RHA1: Redundancies and Convergence Patrauchan, M. A.; Florizone, C.; Dosanjh, M. Journal of Bacteriology, Vol. 187, Issue 12, p. 4050-4063 https://doi.org/10.1128/JB.187.12.4050-4063.2005	journal	June 2005
Phenylacetate Catabolism in Rhodococcus sp. Strain RHA1: a Central Pathway for Degradation of Aromatic Compounds Navarro-Llorens, J. M.; Patrauchan, M. A.; Stewart, G. R. Journal of Bacteriology, Vol. 187, Issue 13 https://doi.org/10.1128/JB.187.13.4497-4504.2005	journal	June 2005
Lignin Biosynthesis Boerjan, Wout; Ralph, John; Baucher, Marie Annual Review of Plant Biology, Vol. 54, Issue 1, p. 519-546 https://doi.org/10.1146/annurev.arplant.54.031902.134938	journal	June 2003
Recent Development in Chemical Depolymerization of Lignin: A Review Wang, Hai; Tucker, Melvin; Ji, Yun Journal of Applied Chemistry, Vol. 2013, p. 1-9 https://doi.org/10.1155/2013/838645	journal	January 2013
Comparative genome analysis of lignin biosynthesis gene families across the plant kingdom Xu, Zhanyou; Zhang, Dandan; Hu, Jun BMC Bioinformatics, Vol. 10, Issue Suppl 11 https://doi.org/10.1186/1471-2105-10-S11-S3	journal	January 2009
Lignin monomer composition affects Arabidopsis cell-wall degradability after liquid hot water pretreatment Li, Xu; Ximenes, Eduardo; Kim, Youngmi Biotechnology for Biofuels, Vol. 3, Issue 1 https://doi.org/10.1186/1754-6834-3-27	journal	January 2010
Characterization and genomic analysis of kraft lignin biodegradation by the beta-proteobacterium Cupriavidus basilensis B-8 Shi, Yan; Chai, Liyuan; Tang, Chongjian Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-1	journal	January 2013
Influence of bark on fuel ethanol production from steam-pretreated spruce Frankó, Balázs; Galbe, Mats; Wallberg, Ola Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0199-x	journal	January 2015
Connecting lignin-degradation pathway with pre-treatment inhibitor sensitivity of Cupriavidus necator Wang, Wei; Yang, Shihui; Hunsinger, Glendon B. Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00247	journal	May 2014
Biomass feedstock: diversity as a solution Yuan, Joshua S.; Wang, Xinwang; Stewart, C. Neal Biofuels, Vol. 2, Issue 5 https://doi.org/10.4155/bfs.11.135	journal	September 2011

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Combinatorial pretreatment and fermentation optimization enabled a record yield on lignin bioconversion Liu, Zhi-Hua; Xie, Shangxian; Lin, Furong Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1021-3	journal	January 2018
Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus Le, Rosemary K.; Das, Parthapratim; Mahan, Kristina M. AMB Express, Vol. 7, Issue 1 https://doi.org/10.1186/s13568-017-0484-0	journal	September 2017
Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production Martins-Santana, Leonardo; Nora, Luisa C.; Sanches-Medeiros, Ananda Frontiers in Bioengineering and Biotechnology, Vol. 6 https://doi.org/10.3389/fbioe.2018.00117	journal	October 2018
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Synergistic maximization of the carbohydrate output and lignin processability by combinatorial pretreatment Liu, Zhi-Hua; Olson, Michelle L.; Shinde, Somnath Green Chem., Vol. 19, Issue 20 https://doi.org/10.1039/c7gc02057k	journal	January 2017
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