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Title: Cooperative valorization of lignin and residual sugar to polyhydroxyalkanoate (PHA) for enhanced yield and carbon utilization in biorefineries

Abstract

Lignin valorization is essential for a sustainable and economically viable biorefinery. Despite the recent efforts, it is still unclear how the reactivity of native lignin can be improved by pretreatment in a biorefinery and how different components in biorefinery residue especially residual sugar contribute to bioconversion in biorefineries. In the present study, co-utilization of lignin and limited glucose was first evaluated and proved to facilitate lignin conversion and polyhydroxyalkanoate (PHA) production. A new integrated biorefinery was then designed to cooperatively valorize lignin and residual sugar for improving the PHA yield and utilization efficiency of biorefinery residue. By the design of integrated biorefinery, the glucose and xylose yield were 91% and 73%, respectively, which increased by 18% and 12% compared with that only using sodium hydroxide pretreatment. Approximately 85% of the lignin was dissolved into a liquid stream with the integrated biorefinery, corresponding to an increase of 69% compared with that only using sodium hydroxide pretreatment. More than 70% of the residual sugar was released from the biorefinery residue, producing the soluble lignin stream that contains both lignin and residual sugar for synergistic bioconversion. Bioconversion of the soluble lignin stream with Pseudomonas putida KT2440 produced 1.5 g l -1PHA, representing themore » record titer of PHA from biorefinery residue. The lignin characterization results from nuclear magnetic resonance and gel-permeation chromatography showed that the integrated process significantly reduced the lignin molecular weight, broke down more β-O-4 and β-5 linkages, and enriched the H-lignin content. Alongside the increased residual sugar concentration, the bioconversion performance of soluble lignin to PHA was significantly improved. Altogether, the integrated biorefinery increased the fermentable sugar yield and improved the PHA production from biorefinery waste by cooperative valorization of lignin and residual sugar, which shows potential advantages for biorefinery sustainability.« less

Authors:
 [1];  [2];  [2]; ORCiD logo [3]; ORCiD logo [4];  [1];  [1];  [1];  [1]
  1. Texas A & M Univ., College Station, TX (United States)
  2. Univ. of Tennessee, Knoxville, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee Inst. of Agriculture, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1526374
Alternate Identifier(s):
OSTI ID: 1525493
Grant/Contract Number:  
AC05-00OR22725; EE0006112; EE0007104; EE0008250
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Sustainable Energy & Fuels
Additional Journal Information:
Journal Volume: TBD; Journal Issue: TBD; Journal ID: ISSN 2398-4902
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Liu, Zhi-hua, Shinde, Somnath, Hao, Naijia, Yoo, Chang Geun, Ragauskas, Arthur J., Xie, Shangxian, Lin, Furong, Li, Man, and Yuan, Joshua S. Cooperative valorization of lignin and residual sugar to polyhydroxyalkanoate (PHA) for enhanced yield and carbon utilization in biorefineries. United States: N. p., 2019. Web. doi:10.1039/c9se00021f.
Liu, Zhi-hua, Shinde, Somnath, Hao, Naijia, Yoo, Chang Geun, Ragauskas, Arthur J., Xie, Shangxian, Lin, Furong, Li, Man, & Yuan, Joshua S. Cooperative valorization of lignin and residual sugar to polyhydroxyalkanoate (PHA) for enhanced yield and carbon utilization in biorefineries. United States. doi:10.1039/c9se00021f.
Liu, Zhi-hua, Shinde, Somnath, Hao, Naijia, Yoo, Chang Geun, Ragauskas, Arthur J., Xie, Shangxian, Lin, Furong, Li, Man, and Yuan, Joshua S. Mon . "Cooperative valorization of lignin and residual sugar to polyhydroxyalkanoate (PHA) for enhanced yield and carbon utilization in biorefineries". United States. doi:10.1039/c9se00021f. https://www.osti.gov/servlets/purl/1526374.
@article{osti_1526374,
title = {Cooperative valorization of lignin and residual sugar to polyhydroxyalkanoate (PHA) for enhanced yield and carbon utilization in biorefineries},
author = {Liu, Zhi-hua and Shinde, Somnath and Hao, Naijia and Yoo, Chang Geun and Ragauskas, Arthur J. and Xie, Shangxian and Lin, Furong and Li, Man and Yuan, Joshua S.},
abstractNote = {Lignin valorization is essential for a sustainable and economically viable biorefinery. Despite the recent efforts, it is still unclear how the reactivity of native lignin can be improved by pretreatment in a biorefinery and how different components in biorefinery residue especially residual sugar contribute to bioconversion in biorefineries. In the present study, co-utilization of lignin and limited glucose was first evaluated and proved to facilitate lignin conversion and polyhydroxyalkanoate (PHA) production. A new integrated biorefinery was then designed to cooperatively valorize lignin and residual sugar for improving the PHA yield and utilization efficiency of biorefinery residue. By the design of integrated biorefinery, the glucose and xylose yield were 91% and 73%, respectively, which increased by 18% and 12% compared with that only using sodium hydroxide pretreatment. Approximately 85% of the lignin was dissolved into a liquid stream with the integrated biorefinery, corresponding to an increase of 69% compared with that only using sodium hydroxide pretreatment. More than 70% of the residual sugar was released from the biorefinery residue, producing the soluble lignin stream that contains both lignin and residual sugar for synergistic bioconversion. Bioconversion of the soluble lignin stream with Pseudomonas putida KT2440 produced 1.5 g l-1PHA, representing the record titer of PHA from biorefinery residue. The lignin characterization results from nuclear magnetic resonance and gel-permeation chromatography showed that the integrated process significantly reduced the lignin molecular weight, broke down more β-O-4 and β-5 linkages, and enriched the H-lignin content. Alongside the increased residual sugar concentration, the bioconversion performance of soluble lignin to PHA was significantly improved. Altogether, the integrated biorefinery increased the fermentable sugar yield and improved the PHA production from biorefinery waste by cooperative valorization of lignin and residual sugar, which shows potential advantages for biorefinery sustainability.},
doi = {10.1039/c9se00021f},
journal = {Sustainable Energy & Fuels},
issn = {2398-4902},
number = TBD,
volume = TBD,
place = {United States},
year = {2019},
month = {6}
}

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    Works referencing / citing this record:

    Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose
    journal, March 2012

    • Zhao, Xuebing; Zhang, Lihua; Liu, Dehua
    • Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 4
    • DOI: 10.1002/bbb.1331

    Pretreatment: the key to unlocking low-cost cellulosic ethanol
    journal, January 2008

    • Yang, Bin; Wyman, Charles E.
    • Biofuels, Bioproducts and Biorefining, Vol. 2, Issue 1
    • DOI: 10.1002/bbb.49

    Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose
    journal, January 2004

    • Yang, Bin; Wyman, Charles E.
    • Biotechnology and Bioengineering, Vol. 86, Issue 1
    • DOI: 10.1002/bit.20043

    High cell density cultivation of Pseudomonas putida KT2440 using glucose without the need for oxygen enriched air supply : Fed Batch Bioprocess for
    journal, November 2014

    • Davis, Reeta; Duane, Gearoid; Kenny, Shane T.
    • Biotechnology and Bioengineering, Vol. 112, Issue 4
    • DOI: 10.1002/bit.25474

    Lignin Depolymerization and Conversion A Review of Thermochemical Methods
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    • Pandey, M. P.; Kim, C. S.
    • Chemical Engineering & Technology, Vol. 34, Issue 1, p. 29-41
    • DOI: 10.1002/ceat.201000270

    Optimization of C:N ratio and minimal initial carbon source for poly(3-hydroxybutyrate) production by Bacillus megaterium
    journal, December 2009

    • Faccin, D��bora Jung Luvizetto; Martins, Ivana; Cardozo, Nilo S��rgio Medeiros
    • Journal of Chemical Technology & Biotechnology, Vol. 84, Issue 12
    • DOI: 10.1002/jctb.2240

    Physiological states and energetic adaptation during growth of Pseudomonas putida mt-2 on glucose
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    • Latrach Tlemçani, Leith; Corroler, David; Barillier, Daniel
    • Archives of Microbiology, Vol. 190, Issue 2
    • DOI: 10.1007/s00203-008-0380-8

    Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass
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    • Klinke, H. B.; Thomsen, A. B.; Ahring, B. K.
    • Applied Microbiology and Biotechnology, Vol. 66, Issue 1, p. 10-26
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    journal, July 2010


    Formation of degradation compounds from lignocellulosic biomass in the biorefinery: sugar reaction mechanisms
    journal, February 2014


    Enzymatic conversion of lignin into renewable chemicals
    journal, December 2015


    Opportunities and challenges in biological lignin valorization
    journal, December 2016


    Monitoring differences in gene expression levels and polyhydroxyalkanoate (PHA) production in Pseudomonas putida KT2440 grown on different carbon sources
    journal, December 2010


    Identification and characterization of a FAD-dependent putrescine N-hydroxylase (GorA) from Gordonia rubripertincta CWB2
    journal, December 2016

    • Esuola, Catherine O.; Babalola, Olubukola O.; Heine, Thomas
    • Journal of Molecular Catalysis B: Enzymatic, Vol. 134
    • DOI: 10.1016/j.molcatb.2016.08.003

    Chemical modification of lignins: Towards biobased polymers
    journal, July 2014


    Biological valorization strategies for converting lignin into fuels and chemicals
    journal, June 2017


    Identifying and creating pathways to improve biological lignin valorization
    journal, May 2019


    Biotechnological opportunities with the β-ketoadipate pathway
    journal, December 2012


    Biorefinery Scheme for Residual Biomass Using Autohydrolysis and Organosolv Stages for Oligomers and Bioethanol Production
    journal, September 2016


    Production and Characterization of Polyhydroxyalkanoate from Lignin Derivatives by Pandoraea sp. ISTKB
    journal, December 2017


    Hydrogen Peroxide-Assisted Sodium Carbonate Pretreatment for the Enhancement of Enzymatic Saccharification of Corn Stover
    journal, November 2015


    Advanced Chemical Design for Efficient Lignin Bioconversion
    journal, January 2017


    Alkaline Pretreatment Severity Leads to Different Lignin Applications in Sugar Cane Biorefineries
    journal, June 2017

    • de Menezes, Fabrícia Farias; Rencoret, Jorge; Nakanishi, Simone Coelho
    • ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 7
    • DOI: 10.1021/acssuschemeng.7b00265

    Codesign of Combinatorial Organosolv Pretreatment (COP) and Lignin Nanoparticles (LNPs) in Biorefineries
    journal, December 2018


    Bacterial Poly(hydroxyalkanoates) as a Source of Chiral Hydroxyalkanoic Acids
    journal, July 2005

    • Ren, Qun; Grubelnik, Andreas; Hoerler, Mirjam
    • Biomacromolecules, Vol. 6, Issue 4
    • DOI: 10.1021/bm050187s

    Progress in Green Polymer Composites from Lignin for Multifunctional Applications: A Review
    journal, April 2014

    • Thakur, Vijay Kumar; Thakur, Manju Kumari; Raghavan, Prasanth
    • ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 5
    • DOI: 10.1021/sc500087z

    Physical and Chemical Characterizations of Corn Stover from Leading Pretreatment Methods and Effects on Enzymatic Hydrolysis
    journal, December 2014

    • Liu, Zhi-Hua; Qin, Lei; Li, Bing-Zhi
    • ACS Sustainable Chemistry & Engineering, Vol. 3, Issue 1
    • DOI: 10.1021/sc500637c

    A novel integrated biological process for cellulosic ethanol production featuring high ethanol productivity, enzyme recycling and yeast cells reuse
    journal, January 2012

    • Jin, Mingjie; Gunawan, Christa; Uppugundla, Nirmal
    • Energy & Environmental Science, Vol. 5, Issue 5
    • DOI: 10.1039/c2ee03058f

    Lignin to lipid bioconversion by oleaginous Rhodococci
    journal, January 2013

    • Kosa, Matyas; Ragauskas, Arthur J.
    • Green Chemistry, Vol. 15, Issue 8
    • DOI: 10.1039/c3gc40434j

    Towards lignin consolidated bioprocessing: simultaneous lignin depolymerization and product generation by bacteria
    journal, January 2015

    • Salvachúa, Davinia; Karp, Eric M.; Nimlos, Claire T.
    • Green Chemistry, Vol. 17, Issue 11
    • DOI: 10.1039/c5gc01165e

    Synergistic enzymatic and microbial lignin conversion
    journal, January 2016

    • Zhao, Cheng; Xie, Shangxian; Pu, Yunqiao
    • Green Chemistry, Vol. 18, Issue 5
    • DOI: 10.1039/c5gc01955a

    Lignin valorization through integrated biological funneling and chemical catalysis
    journal, August 2014

    • Linger, J. G.; Vardon, D. R.; Guarnieri, M. T.
    • Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018
    • DOI: 10.1073/pnas.1410657111

    The Crc protein inhibits the production of polyhydroxyalkanoates in P seudomonas putida under balanced carbon/nitrogen growth conditions : Crc control of PHA production in
    journal, November 2013

    • La Rosa, Ruggero; de la Peña, Fernando; Prieto, María Axiliadora
    • Environmental Microbiology, Vol. 16, Issue 1
    • DOI: 10.1111/1462-2920.12303

    Lignin Valorization: Improving Lignin Processing in the Biorefinery
    journal, May 2014

    • Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
    • Science, Vol. 344, Issue 6185, p. 1246843-1246843
    • DOI: 10.1126/science.1246843

    Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose
    journal, January 2009

    • Kristensen, Jan B.; Felby, Claus; Jørgensen, Henning
    • Biotechnology for Biofuels, Vol. 2, Issue 1
    • DOI: 10.1186/1754-6834-2-11

    Controlling accumulation of fermentation inhibitors in biorefinery recycle water using microbial fuel cells
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    • Borole, Abhijeet P.; Mielenz, Jonathan R.; Vishnivetskaya, Tatiana A.
    • Biotechnology for Biofuels, Vol. 2, Issue 1
    • DOI: 10.1186/1754-6834-2-7

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    • Biotechnology for Biofuels, Vol. 10, Issue 1
    • DOI: 10.1186/s13068-017-0757-5

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    • Biotechnology for Biofuels, Vol. 11, Issue 1
    • DOI: 10.1186/s13068-018-1240-7

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    journal, September 2018

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    • Biotechnology for Biofuels, Vol. 11, Issue 1
    • DOI: 10.1186/s13068-018-1262-1

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    journal, September 2017