Atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints

Xiao, Xiaoyin; Miller, Lance L.; Parchert, Kylea J.; Hayes, Dulce; Hochrein, James M.

doi:10.1002/rcm.7601

Atmospheric solids analysis probe mass spectrometry for the rapid identification of pollens and semi-quantification of flavonoid fingerprints

Journal Article · Wed Jun 08 00:00:00 EDT 2016 · Rapid Communications in Mass Spectrometry

DOI:https://doi.org/10.1002/rcm.7601· OSTI ID:1259863

Xiao, Xiaoyin ^[1]; Miller, Lance L. ^[1]; Parchert, Kylea J. ^[1]; Hayes, Dulce ^[1]; Hochrein, James M. ^[1]

Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

From allergies to plant reproduction, pollens have important impacts on the health of human and plant populations, yet identification of pollen grains remains difficult and time-consuming. Low-volatility flavonoids generated from pollens cannot be easily characterized and quantified with current analytical techniques. Here we demonstrate the novel use of atmospheric solids analysis probe mass spectrometry (ASAP-MS) for the characterization of flavonoids in pollens. Flavonoid patterns were generated for pollens collected from different plant types (trees and bushes) in addition to bee pollens from distinct geographic regions. Standard flavonoids (kaempferol and rhamnazin) and those produced from pollens were compared and assessed with ASAP-MS using low-energy collision MS/MS. Results for a semi-quantitative method for assessing the amount of a flavonoid in pollens are also presented.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1259863

Report Number(s):: SAND--2015-5271J; 594518

Journal Information:: Rapid Communications in Mass Spectrometry, Journal Name: Rapid Communications in Mass Spectrometry Journal Issue: 13 Vol. 30; ISSN 0951-4198

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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