Beyond the wall: High-throughput quantification of plant soluble and cell-wall bound phenolics by liquid chromatography tandem mass spectrometry

Cocuron, Jean-Christophe; Casas, Maria Isabel; Yang, Fan; Grotewold, Erich; Alonso, Ana Paula

doi:10.1016/j.chroma.2018.12.059

Title: Beyond the wall: High-throughput quantification of plant soluble and cell-wall bound phenolics by liquid chromatography tandem mass spectrometry

Journal Article · Wed Dec 26 00:00:00 EST 2018 · Journal of Chromatography

DOI:https://doi.org/10.1016/j.chroma.2018.12.059· OSTI ID:1658576

Cocuron, Jean-Christophe ^[1]; Casas, Maria Isabel ^[2]; Yang, Fan ^[3]; Grotewold, Erich ^[4];

^[1]

Univ. of North Texas, Denton, TX (United States)
NaPro Research, LLC, Washington, DC (United States)
Benson Hill Biosystems, St. Louis, MO (United States)
Michigan State Univ., East Lansing, MI (United States)

Plants accumulate several thousand of phenolic compounds, including lignins and flavonoids, which are mainly synthesized through the phenylpropanoid pathway, and play important roles in plant growth and adaptation. A novel high-throughput ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was established to quantify the levels of 19 flavonoids and 15 other phenolic compounds, including acids, aldehydes, and alcohols. The chromatographic separation was performed in 10 minutes, allowing for the resolution of isomers such as 3-, 4-, and 5-chlorogenic acids, 4-hydroxybenzoic and salicylic acids, isoorientin and orientin, and luteolin and kaempferol. The linearity range for each compound was found to be in the low fmol to the high pmol. Furthermore, this UHPLC-MS/MS approach was shown to be very sensitive with limits of detection between 1.5 attomoles to 300 fmoles, and limits of quantification between 5 attomoles to 1,000 fmoles. Extracts from maize seedlings were used to assess the robustness of the method in terms of recovery efficiency, matrix effect, and accuracy. The biological matrix did not suppress the signal for 32 out of the 34 metabolites under investigation. Additionally, the majority of the analytes were recovered from the biological samples with an efficiency above 75 %. All flavonoids and other phenolic compounds had an intra- and inter-day accuracy within a ± 20 % range, except for coniferyl alcohol and vanillic acid. Finally, the quantification of flavonoids, free and cell wall-bound phenolics in seedlings from two maize lines with contrasting phenolic content was successfully achieved using this methodology.

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Research Organization:: Univ. of North Texas, Denton, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0016490

OSTI ID:: 1658576

Journal Information:: Journal of Chromatography, Vol. 1589; ISSN 0021-9673

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 28 works

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Title: Beyond the wall: High-throughput quantification of plant soluble and cell-wall bound phenolics by liquid chromatography tandem mass spectrometry

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