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Title: Identification of Porphyrin-Silica Composite Nanoparticles using Atmospheric Solids Analysis Probe Mass Spectrometry

Journal Article · · MRS Advances
DOI:https://doi.org/10.1557/adv.2019.217· OSTI ID:1515214
 [1];  [1];  [1];  [1];  [1];  [2];  [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
  2. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Univ. of New Mexico, Albuquerque, NM (United States)
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Porphyrins are critical pigments involved in biological energy transduction processes. Their abilities to absorb light, then convert it to energy, have raised the interest of using porphyrin nanoparticles as photosensitizers in photodynamic therapy. A current study showed that self- assembled porphyrin-silica composite nanoparticles can selectively destroy tumor cells, but detection of the cellular uptake of porphyrin-silica composite nanoparticles was limited to imaging microscopy. In this work, we developed a novel method to rapidly identify porphyrin-silica composite nanoparticles using Atmospheric Solids Analysis Probe-Mass Spectrometry (ASAP-MS). ASAP-MS can directly analyze complex mixtures without the need for sample preparation. Porphyrin-silica composite nanoparticles were vaporized using heated nitrogen desolvation gas, and their thermo-profiles were examined to identify distinct mass- to-charge (M/Z) signatures. HeLa cells were incubated in growth media containing the nanoparticles, and after sufficient washing to remove residual nanoparticles, the cell suspension was loaded onto the end of ASAP glass capillary probe. Upon heating, HeLa cells were degraded and porphyrin-silica composite nanoparticles were released. Vaporized nanoparticles were ionized and detected by MS. Furthermore, the cellular uptake of porphyrin-silica composite nanoparticles was identified using this ASAP-MS method.

Research Organization:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
Grant/Contract Number:
AC04-94AL85000
OSTI ID:
1515214
Report Number(s):
SAND-2019-4470J; applab; 674884
Journal Information:
MRS Advances, Vol. 4, Issue 38-39; ISSN 2059-8521
Publisher:
Materials Research Society (MRS)Copyright Statement
Country of Publication:
United States
Language:
English

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Thermal degradation of β-carotene studied by ion mobility atmospheric solid analysis probe mass spectrometry: full product pattern and selective ionization enhancement: Degradation of low volatility compounds journal March 2016
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pH-Dependent Assembly of Porphyrin–Silica Nanocomposites and Their Application in Targeted Photodynamic Therapy journal October 2017
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Synthesis of Self-Assembled Porphyrin Nanoparticle Photosensitizers journal March 2018
Self-Assembled One-Dimensional Porphyrin Nanostructures with Enhanced Photocatalytic Hydrogen Generation journal December 2017
Atmospheric pressure solid analysis probe coupled to quadrupole-time of flight mass spectrometry as a tool for screening and semi-quantitative approach of polycyclic aromatic hydrocarbons, nitro-polycyclic aromatic hydrocarbons and oxo-polycyclic aromatic hydrocarbons in complex matrices journal January 2015
Porous One-Dimensional Nanostructures through Confined Cooperative Self-Assembly journal December 2011
Pressure-Tuned Structure and Property of Optically Active Nanocrystals journal January 2016
Morphology-Controlled Self-Assembly and Synthesis of Photocatalytic Nanocrystals journal November 2014
Use of an Atmospheric Solids Analysis Probe (ASAP) for High Throughput Screening of Biological Fluids: Preliminary Applications on Urine and Bile journal July 2010
Stress-Induced Nanoparticle Crystallization journal May 2014
Identification of Ritual Blood in African Artifacts Using TOF-SIMS and Synchrotron Radiation Microspectroscopies journal December 2007
Interfacial Self-Assembly Driven Formation of Hierarchically Structured Nanocrystals with Photocatalytic Activity journal December 2013
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36 MATERIALS SCIENCE