Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging

Klein, Adam T.; Yagnik, Gargey B.; Hohenstein, Jessica D.; Ji, Zhiyuan; Zi, Jiachen; Reichert, Malinda D.; MacIntosh, Gustavo C.; Yang, Bing; Peters, Reuben J.; Vela, Javier; Lee, Young Jin

doi:10.1021/acs.analchem.5b00459

Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging

Journal Article · Mon Apr 27 00:00:00 EDT 2015 · Analytical Chemistry

DOI:https://doi.org/10.1021/acs.analchem.5b00459· OSTI ID:1227451

Klein, Adam T. ^[1]; Yagnik, Gargey B. ^[1]; Hohenstein, Jessica D. ^[2]; Ji, Zhiyuan ^[3]; Zi, Jiachen ^[2]; Reichert, Malinda D. ^[2]; MacIntosh, Gustavo C. ^[2]; Yang, Bing ^[2]; Peters, Reuben J. ^[2]; Vela, Javier ^[2]; Lee, Young Jin ^[1]

Iowa State Univ., Ames, IA (United States); Ames Lab., Ames, IA (United States)
Iowa State Univ., Ames, IA (United States)
Shanghai Jiao Tong Univ., Shanghai (China); Iowa State Univ., Ames, IA (United States)

Mass spectrometry imaging (MSI) is an emerging technology for high-resolution plant biology. It has been utilized to study plant–pest interactions, but limited to the surface interfaces. Here we expand the technology to explore the chemical interactions occurring inside the plant tissues. Two sample preparation methods, imprinting and fracturing, were developed and applied, for the first time, to visualize internal metabolites of leaves in matrix-assisted laser desorption ionization (MALDI)-MSI. This is also the first time nanoparticle-based ionization was implemented to ionize diterpenoid phytochemicals that were difficult to analyze with traditional organic matrices. The interactions between rice–bacterium and soybean–aphid were investigated as two model systems to demonstrate the capability of high-resolution MSI based on MALDI. Localized molecular information on various plant- or pest-derived chemicals provided valuable insight for the molecular processes occurring during the plant–pest interactions. Basically, salicylic acid and isoflavone based resistance was visualized in the soybean–aphid system and antibiotic diterpenoids in rice–bacterium interactions.

View Accepted Manuscript (DOE)

Research Organization:: Ames Laboratory (AMES), Ames, IA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-07CH11358

OSTI ID:: 1227451

Report Number(s):: IS-J--8639

Journal Information:: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 10 Vol. 87; ISSN 0003-2700

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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