Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ

Sturtevant, Drew; Lee, Young -Jin; Chapman, Kent D.

doi:10.1016/j.copbio.2015.10.004

Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ

Journal Article · Sat Nov 21 23:00:00 EST 2015 · Current Opinion in Biotechnology

DOI:https://doi.org/10.1016/j.copbio.2015.10.004· OSTI ID:1249347

Sturtevant, Drew ^[1]; Lee, Young -Jin ^[2]; Chapman, Kent D. ^[1]

Univ. of North Texas, Denton, TX (United States)
Iowa State Univ., Ames, IA (United States); Ames Lab., Ames, IA (United States)

Direct visualization of plant tissues by matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has revealed key insights into the localization of metabolites in situ. Recent efforts have determined the spatial distribution of primary and secondary metabolites in plant tissues and cells. Strategies have been applied in many areas of metabolism including isotope flux analyses, plant interactions, and transcriptional regulation of metabolite accumulation. Technological advances have pushed achievable spatial resolution to subcellular levels and increased instrument sensitivity by several orders of magnitude. Furthermore, it is anticipated that MALDI-MSI and other MSI approaches will bring a new level of understanding to metabolomics as scientists will be encouraged to consider spatial heterogeneity of metabolites in descriptions of metabolic pathway regulation.

View Accepted Manuscript (DOE)

Research Organization:: Ames Laboratory (AMES), Ames, IA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-07CH11358

OSTI ID:: 1249347

Report Number(s):: IS-J--8967; PII: S0958166915001421

Journal Information:: Current Opinion in Biotechnology, Journal Name: Current Opinion in Biotechnology Journal Issue: C Vol. 37; ISSN 0958-1669

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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