A Method to Determine Lysine Acetylation Stoichiometries

Journal Article · Wed Jan 01 00:00:00 EST 2014 · International Journal of Proteomics

DOI:https://doi.org/10.1155/2014/730725· OSTI ID:1198079

Nakayasu, Ernesto S. ^[1]; Wu, Si ^[2]; Sydor, Michael A. ^[3]; Shukla, Anil K. ^[3]; Weitz, Karl K. ^[3]; Moore, Ronald J. ^[3]; Hixson, Kim K. ^[2]; Kim, Jong-Seo ^[4]; Petyuk, Vladislav A. ^[3]; Monroe, Matthew E. ^[3]; Pasa-Tolic, Ljiljiana ^[2]; Qian, Wei-Jun ^[3]; Smith, Richard D. ^[3]; Adkins, Joshua N. ^[3]; Ansong, Charles ^[3]

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1. Biological Science Division and Environmental, Pacific Northwest National Laboratory, Richland, WA 99352, USA, Bindley Bioscience Center, Discovery Park, Purdue University, West Lafayette, IN 47907, USA
2. Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA
3. Biological Science Division and Environmental, Pacific Northwest National Laboratory, Richland, WA 99352, USA
4. Biological Science Division and Environmental, Pacific Northwest National Laboratory, Richland, WA 99352, USA, Institute for Basic Science, Seoul National University, Seoul 151-747, Republic of Korea

Lysine acetylation is a common protein posttranslational modification that regulates a variety of biological processes. A major bottleneck to fully understanding the functional aspects of lysine acetylation is the difficulty in measuring the proportion of lysine residues that are acetylated. Here we describe a mass spectrometry method using a combination of isotope labeling and detection of a diagnostic fragment ion to determine the stoichiometry of protein lysine acetylation. Using this technique, we determined the modification occupancy for ~750 acetylated peptides from mammalian cell lysates. Furthermore, the acetylation on N-terminal tail of histone H4 was cross-validated by treating cells with sodium butyrate, a potent deacetylase inhibitor, and comparing changes in stoichiometry levels measured by our method with immunoblotting measurements. Of note we observe that acetylation stoichiometry is high in nuclear proteins, but very low in mitochondrial and cytosolic proteins. In summary, our method opens new opportunities to study in detail the relationship of lysine acetylation levels of proteins with their biological functions.

• Sponsoring Organization: USDOE
• Grant/Contract Number: AC05-76-RLO1830
• OSTI ID: 1198079
• Journal Information: International Journal of Proteomics, Journal Name: International Journal of Proteomics Vol. 2014; ISSN 2090-2166
• Publisher: Hindawi Publishing Corporation
• Country of Publication: Country unknown/Code not available
• Language: English