A Method to determine lysine acetylation stoichiometries
Journal Article
·
· International Journal of Proteomics, 2014:Article No. 730725
A major bottleneck to fully understanding the functional aspects of lysine acetylation is the lack of stoichiometry information. Here we describe a mass spectrometry method using a combination of isotope labeling and detection of a diagnostic fragment ion to determine the stoichiometry of lysine acetylation on proteins globally. Using this technique, we determined the modification occupancy on hundreds of acetylated peptides from cell lysates and cross-validated the measurements via immunoblotting.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1171295
- Report Number(s):
- PNNL-SA-96571; 42294; 400412000
- Journal Information:
- International Journal of Proteomics, 2014:Article No. 730725, Journal Name: International Journal of Proteomics, 2014:Article No. 730725
- Country of Publication:
- United States
- Language:
- English
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