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Mutant E. coli strain with increased succinic acid production

Patent ·
OSTI ID:1176700

A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

Research Organization:
University Of Chicago, Chicago, IL (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-31109-ENG-38
Assignee:
The University of Chicago (Chicago, IL)
Patent Number(s):
RE37393
Application Number:
09/429,693; 5,770,435
OSTI ID:
1176700
Country of Publication:
United States
Language:
English

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