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Title: Mutant E. coli strain with increased succinic acid production

Abstract

A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which as been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

Inventors:
 [1];  [2];  [3]
  1. (Warrenville, IL)
  2. (Plainfield, IL)
  3. (Woodridge, IL)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL
OSTI Identifier:
871646
Patent Number(s):
US 5770435
Assignee:
University of Chicago (Chicago, IL) ANL
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
mutant; coli; strain; increased; succinic; acid; production; method; isolating; producing; bacteria; provided; comprising; increasing; biomass; organism; lacks; ability; catabolize; pyruvate; subjecting; glucose-rich; medium; anaerobic; environment; enable; pyruvate-catabolizing; mutants; grow; provides; produces; amounts; derived; parent; lacked; genes; formate; lyase; lactate; dehydrogenase; belongs; acid production; succinic acid; provided comprising; acid producing; producing bacteria; catabolize pyruvate; comprising increasing; coli strain; increased succinic; isolating succinic; /435/

Citation Formats

Donnelly, Mark, Millard, Cynthia S., and Stols, Lucy. Mutant E. coli strain with increased succinic acid production. United States: N. p., 1998. Web.
Donnelly, Mark, Millard, Cynthia S., & Stols, Lucy. Mutant E. coli strain with increased succinic acid production. United States.
Donnelly, Mark, Millard, Cynthia S., and Stols, Lucy. Thu . "Mutant E. coli strain with increased succinic acid production". United States. https://www.osti.gov/servlets/purl/871646.
@article{osti_871646,
title = {Mutant E. coli strain with increased succinic acid production},
author = {Donnelly, Mark and Millard, Cynthia S. and Stols, Lucy},
abstractNote = {A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which as been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1998},
month = {Thu Jan 01 00:00:00 EST 1998}
}

Patent:

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