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Title: Mutant E. coli strain with increased succinic acid production

Abstract

A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

Inventors:
 [1];  [2];  [3]
  1. Warrenville, IL
  2. Plainfield, IL
  3. Woodridge, IL
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
OSTI Identifier:
875302
Patent Number(s):
US RE37393
Assignee:
University of Chicago (Chicago, IL)
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
mutant; coli; strain; increased; succinic; acid; production; method; isolating; producing; bacteria; provided; comprising; increasing; biomass; organism; lacks; ability; catabolize; pyruvate; subjecting; glucose-rich; medium; anaerobic; environment; enable; pyruvate-catabolizing; mutants; grow; provides; produces; amounts; derived; parent; lacked; genes; formate; lyase; lactate; dehydrogenase; belongs; acid production; succinic acid; provided comprising; acid producing; producing bacteria; catabolize pyruvate; comprising increasing; coli strain; increased succinic; isolating succinic; /435/

Citation Formats

Donnelly, Mark, Millard, Cynthia S, and Stols, Lucy. Mutant E. coli strain with increased succinic acid production. United States: N. p., 2002. Web.
Donnelly, Mark, Millard, Cynthia S, & Stols, Lucy. Mutant E. coli strain with increased succinic acid production. United States.
Donnelly, Mark, Millard, Cynthia S, and Stols, Lucy. 2002. "Mutant E. coli strain with increased succinic acid production". United States. https://www.osti.gov/servlets/purl/875302.
@article{osti_875302,
title = {Mutant E. coli strain with increased succinic acid production},
author = {Donnelly, Mark and Millard, Cynthia S and Stols, Lucy},
abstractNote = {A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.},
doi = {},
url = {https://www.osti.gov/biblio/875302}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}