Production of polyhydroxybutyrate in switchgrass

Somleva, Mariya N.; Snell, Kristi D.; Beaulieu, Julie; Peoples, Oliver P.; Garrison, Bradley; Patterson, Nii

Production of polyhydroxybutyrate in switchgrass

Patent · 16 July 2013

OSTI ID:1176503

Somleva, Mariya N.; Snell, Kristi D.; Beaulieu, Julie; Peoples, Oliver P.; Garrison, Bradley; Patterson, Nii

Transgenic plants, plant material, and plant cells for synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to a as PHB) are provided. Preferred plants that can be genetically engineered to produce PHB include plants that do not normally produce storage products such as oils and carbohydrates, and plants that have a C.sub.4 NAD-malic enzyme photosynthetic pathway. Such plants also advantageously produce lignocellulosic biomass that can be converted into biofuels. An exemplary plant that can be genetically engineered to produce PHB and produce lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include but are not limited to Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.

View Patent

Research Organization:: Metabolix, Inc., Cambridge, MA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC07-01ID14214

Assignee:: Metabolix, Inc. (Cambridge, MA)

Patent Number(s):: 8,487,159

Application Number:: 12/431,428

OSTI ID:: 1176503

Country of Publication:: United States

Language:: English

References (78)

Production of polyhydroxybutyrate in sugarcane Petrasovits, Lars A.; Purnell, Matthew P.; Nielsen, Lars K. Plant Biotechnology Journal, Vol. 5, Issue 1 https://doi.org/10.1111/j.1467-7652.2006.00229.x	journal	January 2007
The Path Forward for Biofuels and Biomaterials Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H. Science, Vol. 311, Issue 5760, p. 484-489 https://doi.org/10.1126/science.1114736	journal	January 2006
Stable Transformation of Soybean Callus by DNA-Coated Gold Particles Christou, Paul; McCabe, Dennis E.; Swain, William F. Plant Physiology, Vol. 87, Issue 3 https://doi.org/10.1104/pp.87.3.671	journal	July 1988
Expression of bacterial poly(3-hydroxybutyrate) synthesis genes in hairy roots of sugar beet (Beta vulgaris L.) Menzel, G.; Harloff, H. -J.; Jung, C. Applied Microbiology and Biotechnology, Vol. 60, Issue 5 https://doi.org/10.1007/s00253-002-1152-z	journal	January 2003
Transfer of foreign genes into intact maize cells with high-velocity microprojectiles Klein, T. M.; Fromm, M.; Weissinger, A. Proceedings of the National Academy of Sciences, Vol. 85, Issue 12 https://doi.org/10.1073/pnas.85.12.4305	journal	June 1988
Bonkers about biofuels Herrera, Stephan Nature Biotechnology, Vol. 24, Issue 7 https://doi.org/10.1038/nbt0706-755	journal	July 2006
Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation. Staub, J. M.; Maliga, P. The Plant Cell, Vol. 4, Issue 1 https://doi.org/10.1105/tpc.4.1.39	journal	January 1992
Parameters Influencing Stable Transformation of Rice Immature Embryos and Recovery of Transgenic Plants using Electric Discharge Particle Acceleration Christou, P. Annals of Botany, Vol. 75, Issue 4 https://doi.org/10.1006/anbo.1995.1038	journal	April 1995
Tissue-specific light-regulated expression directed by the promoter of a C4 gene, maize pyruvate,orthophosphate dikinase, in a C3 plant, rice. Matsuoka, M.; Tada, Y.; Fujimura, T. Proceedings of the National Academy of Sciences, Vol. 90, Issue 20 https://doi.org/10.1073/pnas.90.20.9586	journal	October 1993
Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16 Peoples, O. P.; Sinskey, A. J. Journal of Biological Chemistry, Vol. 264, Issue 26 https://doi.org/10.1016/S0021-9258(19)84824-X	journal	September 1989
Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate- co-3-hydroxyvalerate) copolymer production Slater, Steven; Mitsky, Timothy A.; Houmiel, Kathryn L. Nature Biotechnology, Vol. 17, Issue 10 https://doi.org/10.1038/13711	journal	October 1999
Molecular breeding of switchgrass for use as a biofuel crop Bouton, Joseph H. Current Opinion in Genetics & Development, Vol. 17, Issue 6 https://doi.org/10.1016/j.gde.2007.08.012	journal	December 2007
Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. Nawrath, C.; Poirier, Y.; Somerville, C. Proceedings of the National Academy of Sciences, Vol. 91, Issue 26 https://doi.org/10.1073/pnas.91.26.12760	journal	December 1994
Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants Fromm, Michael E.; Morrish, Fionnuala; Armstrong, Charles Nature Biotechnology, Vol. 8, Issue 9 https://doi.org/10.1038/nbt0990-833	journal	September 1990
Alterations in the Chlamydomonas Plastocyanin Transit Peptide Have Distinct Effects on in VitroImport and in Vivo Protein Accumulation Lawrence, Susan D.; Kindle, Karen L. Journal of Biological Chemistry, Vol. 272, Issue 33 https://doi.org/10.1074/jbc.272.33.20357	journal	August 1997
-Mediated Genetic Transformation of Switchgrass Somleva, M. N.; Tomaszewski, Z.; Conger, B. V. Crop Science, Vol. 42, Issue 6 https://doi.org/10.2135/cropsci2002.2080	journal	January 2002
Analysis of three tissue-specific elements from the wheat Cab-1 enhancer: Functional elements of a Cab enhancer Gotor, Cecilia; Romero, Luis C.; Inouye, Keiko The Plant Journal, Vol. 3, Issue 4 https://doi.org/10.1046/j.1365-313X.1993.03040509.x	journal	April 1993
Firefly luciferase gene: structure and expression in mammalian cells de Wet, J. R.; Wood, K. V.; DeLuca, M. Molecular and Cellular Biology, Vol. 7, Issue 2 https://doi.org/10.1128/mcb.7.2.725-737.1987	journal	February 1987
Protein Trafficking in Plant Cells Della-Cioppa, G.; Kishore, G. M.; Beachy, R. N. Plant Physiology, Vol. 84, Issue 4, p. 965-968 https://doi.org/10.1104/pp.84.4.965	journal	August 1987
Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker for site-directed transformation of chlamydomonas Goldschmidt-Clermont, Michel Nucleic Acids Research, Vol. 19, Issue 15 https://doi.org/10.1093/nar/19.15.4083	journal	January 1991
Poly(β-hydroxybutyrate) production in oilseed leukoplasts of Brassica napus Houmiel, Kathryn L.; Slater, Steven; Broyles, Debra Planta, Vol. 209, Issue 4 https://doi.org/10.1007/s004250050760	journal	October 1999
Engineering of PHB Synthesis Causes Improved Elastic Properties of Flax Fibers Wrobel-Kwiatkowska, M.; Zebrowski, J.; Starzycki, M. Biotechnology Progress, Vol. 23, Issue 1 https://doi.org/10.1021/bp0601948	journal	February 2007
Polyhydroxybutyrate synthesis in transgenic flax Wróbel, Magdalena; Zebrowski, Jacek; Szopa, Jan Journal of Biotechnology, Vol. 107, Issue 1, p. 41-54 https://doi.org/10.1016/j.jbiotec.2003.10.005	journal	January 2004
A novel operon organization involving the genes for chorismate synthase (aromatic biosynthesis pathway) and ribosomal GTPase center proteins (L11, L1, L10, L12: rplKAJL) in cyanobacterium Synechocystis PCC 6803. Schmidt, J.; Bubunenko, M.; Subramanian, A. R. Journal of Biological Chemistry, Vol. 268, Issue 36 https://doi.org/10.1016/S0021-9258(19)74268-9	journal	December 1993
Luciferase reporter gene cassettes for plant gene expression studies Riggs, C. Daniel; Chrispeels, Maarten J. Nucleic Acids Research, Vol. 15, Issue 19 https://doi.org/10.1093/nar/15.19.8115	journal	January 1987
Production of Polyhydroxybutyrate by Polycistronic Expression of Bacterial Genes in Tobacco Plastid Arai, Yuko; Shikanai, Toshiharu; Doi, Yoshiharu Plant and Cell Physiology, Vol. 45, Issue 9 https://doi.org/10.1093/pcp/pch139	journal	September 2004
Isolation of an efficient actin promoter for use in rice transformation. McElroy, D.; Zhang, W.; Cao, J. The Plant Cell, Vol. 2, Issue 2 https://doi.org/10.1105/tpc.2.2.163	journal	February 1990
High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Svab, Z.; Maliga, P. Proceedings of the National Academy of Sciences, Vol. 90, Issue 3 https://doi.org/10.1073/pnas.90.3.913	journal	February 1993
CELLULOSIC ETHANOL: Biofuel Researchers Prepare to Reap a New Harvest Service, R. F. Science, Vol. 315, Issue 5818 https://doi.org/10.1126/science.315.5818.1488	journal	March 2007
Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus. Miao, G. H.; Hirel, B.; Marsolier, M. C. The Plant Cell, Vol. 3, Issue 1 https://doi.org/10.1105/tpc.3.1.11	journal	January 1991
Switchgrass as a biofuels feedstock in the USA Sanderson, M. A.; Adler, P. R.; Boateng, A. A. Canadian Journal of Plant Science, Vol. 86, Issue Special Issue https://doi.org/10.4141/P06-136	journal	December 2006
Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death: Glucocorticoid-inducible cell death McNellis, Timothy W.; Mudgett, Mary Beth; Li, Karen The Plant Journal, Vol. 14, Issue 2 https://doi.org/10.1046/j.1365-313X.1998.00106.x	journal	April 1998
Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. Goff, S. A.; Klein, T. M.; Roth, B. A. The EMBO Journal, Vol. 9, Issue 8 https://doi.org/10.1002/j.1460-2075.1990.tb07431.x	journal	August 1990
Genetic Transformation of Maize Cells by Particle Bombardment Klein, Theodore M.; Kornstein, Laura; Sanford, John C. Plant Physiology, Vol. 91, Issue 1 https://doi.org/10.1104/pp.91.1.440	journal	September 1989
Transgenic Arabidopsis plants can accumulate polyhydroxybutyrate to up to 4% of their fresh weight Bohmert, Karen; Balbo, Ilse; Kopka, Joachim Planta, Vol. 211, Issue 6 https://doi.org/10.1007/s004250000350	journal	November 2000
Light-responsive elements of the tobacco PSI-D gene are located both upstream and within the transcribed region Yamamoto, Yoshiharu Y.; Kondo, Yukiko; Kato, Akira The Plant Journal, Vol. 12, Issue 2 https://doi.org/10.1046/j.1365-313X.1997.12020255.x	journal	August 1997
Vascular-specific expression of the bean GRP 1.8 gene is negatively regulated. Keller, B.; Baumgartner, C. The Plant Cell, Vol. 3, Issue 10 https://doi.org/10.1105/tpc.3.10.1051	journal	October 1991
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter Odell, Joan T.; Nagy, Ferenc; Chua, Nam-Hai Nature, Vol. 313, Issue 6005, p. 810-812 https://doi.org/10.1038/313810a0	journal	February 1985
A novel thiolase-reductase gene fusion promotes the production of polyhydroxybutyrate in Arabidopsis Kourtz, Lauralynn; Dillon, Kevin; Daughtry, Sean Plant Biotechnology Journal, Vol. 3, Issue 4 https://doi.org/10.1111/j.1467-7652.2005.00136.x	journal	July 2005
Plant Regeneraton from Callus Cultures of Switchgrass Denchev, P. D.; Conger, B. V. Crop Science, Vol. 34, Issue 6 https://doi.org/10.2135/cropsci1994.0011183X003400060036x	journal	January 1994
The chlorophyll a/b-binding protein inserts into the thylakoids independent of its cognate transit peptide. Lamppa, G. K. Journal of Biological Chemistry, Vol. 263, Issue 29 https://doi.org/10.1016/S0021-9258(18)68137-2	journal	October 1988
Immunological Characterization and Chloroplast Localization of the Tryptophan Biosynthetic Enzymes of the Flowering Plant Arabidopsis thaliana Zhao, Jianmin; Last, Robert L. Journal of Biological Chemistry, Vol. 270, Issue 11 https://doi.org/10.1074/jbc.270.11.6081	journal	March 1995
Spatio-temporal characterization of polyhydroxybutyrate accumulation in sugarcane Purnell, Matthew P.; Petrasovits, Lars A.; Nielsen, Lars K. Plant Biotechnology Journal, Vol. 5, Issue 1 https://doi.org/10.1111/j.1467-7652.2006.00230.x	journal	January 2007
Net energy of cellulosic ethanol from switchgrass Schmer, M. R.; Vogel, K. P.; Mitchell, R. B. Proceedings of the National Academy of Sciences, Vol. 105, Issue 2 https://doi.org/10.1073/pnas.0704767105	journal	January 2008
Tissue-Specific and Developmental Regulation of Cotton Gene FbL2A (Demonstration of Promoter Activity in Transgenic Plants) Rinehart, J. A.; Petersen, M. W.; John, M. E. Plant Physiology, Vol. 112, Issue 3 https://doi.org/10.1104/pp.112.3.1331	journal	November 1996
Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements Guevara-Garcia, Arturo; Mosqueda-Cano, Gilberto; Arguello-Astorga, Gerardo The Plant Journal, Vol. 4, Issue 3 https://doi.org/10.1046/j.1365-313X.1993.04030495.x	journal	September 1993
Modification of the coding sequence enhances plant expression of insect control protein genes. Perlak, F. J.; Fuchs, R. L.; Dean, D. A. Proceedings of the National Academy of Sciences, Vol. 88, Issue 8 https://doi.org/10.1073/pnas.88.8.3324	journal	April 1991
Stable transformation of tobacco by electroporation: evidence for plasmid concatenation. Riggs, C. D.; Bates, G. W. Proceedings of the National Academy of Sciences, Vol. 83, Issue 15 https://doi.org/10.1073/pnas.83.15.5602	journal	August 1986
Signal peptide analogs derived from two chloroplast precursors interact with the signal recognition system of the chloroplast envelope. Schnell, D. J.; Blobel, G.; Pain, D. Journal of Biological Chemistry, Vol. 266, Issue 5 https://doi.org/10.1016/S0021-9258(18)49993-0	journal	February 1991
Chemically inducible expression of the PHB biosynthetic pathway in Arabidopsis Kourtz, Lauralynn; Dillon, Kevin; Daughtry, Sean Transgenic Research, Vol. 16, Issue 6 https://doi.org/10.1007/s11248-007-9067-1	journal	February 2007
Identification of a Light-Responsive Region of the Nuclear Gene Encoding the B Subunit of Chloroplast Glyceraldehyde 3-Phosphate Dehydrogenase from Arabidopsis thaliana Kwon, H. B.; Park, S. C.; Peng, H. P. Plant Physiology, Vol. 105, Issue 1 https://doi.org/10.1104/pp.105.1.357	journal	May 1994
Plastid Targeting of Polyhydroxybutyrate Biosynthetic Pathway in Tobacco Arai, Yuko; Nakashita, Hideo; Doi, Yoshiharu Plant Biotechnology, Vol. 18, Issue 4 https://doi.org/10.5511/plantbiotechnology.18.289	journal	January 2001
Mutations at the Transit Peptide-Mature Protein Junction Separate Two Cleavage Events during Chloroplast Import of the Chlorophyll a/b-binding Protein Clark, S. E.; Abad, M. S.; Lamppa, G. K. Journal of Biological Chemistry, Vol. 264, Issue 29 https://doi.org/10.1016/S0021-9258(18)71526-3	journal	October 1989
Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells Blochlinger, K.; Diggelmann, H. Molecular and Cellular Biology, Vol. 4, Issue 12 https://doi.org/10.1128/mcb.4.12.2929-2931.1984	journal	December 1984
The Biology and Agronomy of Switchgrass for Biofuels Parrish, David J.; Fike, John H. Critical Reviews in Plant Sciences, Vol. 24, Issue 5-6 https://doi.org/10.1080/07352680500316433	journal	September 2005
Constitutive Expression of the β-Ketothiolase Gene in Transgenic Plants. A Major Obstacle for Obtaining Polyhydroxybutyrate-Producing Plants Bohmert, Karen; Balbo, Ilse; Steinbüchel, Alexander Plant Physiology, Vol. 128, Issue 4 https://doi.org/10.1104/pp.010615	journal	February 2002
T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis Bytebier, B.; Deboeck, F.; De Greve, H. Proceedings of the National Academy of Sciences, Vol. 84, Issue 15 https://doi.org/10.1073/pnas.84.15.5345	journal	August 1987
PHA production, from bacteria to plants Valentin, Henry E.; Broyles, Debra L.; Casagrande, Laura A. International Journal of Biological Macromolecules, Vol. 25, Issue 1-3, p. 303-306 https://doi.org/10.1016/S0141-8130(99)00045-8	journal	June 1999
Tissue-Specific Activity of Two Manganese Superoxide Dismutase Promoters in Transgenic Tobacco Van Camp, W.; Herouart, D.; Willekens, H. Plant Physiology, Vol. 112, Issue 2 https://doi.org/10.1104/pp.112.2.525	journal	October 1996
Transgenic maize plants by tissue electroporation. D'Halluin, K.; Bonne, E.; Bossut, M. The Plant Cell, Vol. 4, Issue 12 https://doi.org/10.1105/tpc.4.12.1495	journal	December 1992
A cassette containing the bar gene of Streptomyces hygroscopicus : a selectable marker for plant transformation White, Janet; Chang, Sheng-Yung P.; Bibb, Maureen J. Nucleic Acids Research, Vol. 18, Issue 4 https://doi.org/10.1093/nar/18.4.1062	journal	January 1990
Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.): significant contents of polyhydroxybutyrate are associated with growth reduction Lössl, A.; Eibl, C.; Harloff, H. -J. Plant Cell Reports, Vol. 21, Issue 9 https://doi.org/10.1007/s00299-003-0610-0	journal	April 2003
Introduction of Bacterial Metabolism into Higher Plants by Polycistronic Transgene Expression Nakashita, Hideo; Arai, Yuko; Shikanai, Toshiharu Bioscience, Biotechnology, and Biochemistry, Vol. 65, Issue 7 https://doi.org/10.1271/bbb.65.1688	journal	January 2001
Production of a Biodegradable Plastic Polymer, Poly‐β‐Hydroxybutyrate, in Transgenic Alfalfa Saruul, Purev; Srienc, Friedrich; Somers, David A. Crop Science, Vol. 42, Issue 3 https://doi.org/10.2135/cropsci2002.9190	journal	May 2002
Switchgrass (Panicum virgatum L.) Somleva, Mariya N. Agrobacterium Protocols Volume 2 https://doi.org/10.1385/1-59745-131-2:65	book	January 2006
Tissue-Specific Expression and Promoter Analysis of the Tobacco ltp1 Gene Canevascini, S.; Caderas, D.; Mandel, T. Plant Physiology, Vol. 112, Issue 2 https://doi.org/10.1104/pp.112.2.513	journal	October 1996
Strcuture and transcription of the nopaline synthase gene region of T-DNA Bevan, Michael; Barnes, Wayne M.; Chilton, Marry-Dell Nucleic Acids Research, Vol. 11, Issue 2 https://doi.org/10.1093/nar/11.2.369	journal	January 1983
Metabolic pathway engineering in cotton: Biosynthesis of polyhydroxybutyrate in fiber cells John, M. E.; Keller, G. Proceedings of the National Academy of Sciences, Vol. 93, Issue 23 https://doi.org/10.1073/pnas.93.23.12768	journal	November 1996
A steroid-inducible gene expression system for plant cells. Schena, M.; Lloyd, A. M.; Davis, R. W. Proceedings of the National Academy of Sciences, Vol. 88, Issue 23 https://doi.org/10.1073/pnas.88.23.10421	journal	December 1991
Stable transformation of plastids in higher plants. Svab, Z.; Hajdukiewicz, P.; Maliga, P. Proceedings of the National Academy of Sciences, Vol. 87, Issue 21 https://doi.org/10.1073/pnas.87.21.8526	journal	November 1990
Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase. McBride, K. E.; Schaaf, D. J.; Daley, M. Proceedings of the National Academy of Sciences, Vol. 91, Issue 15 https://doi.org/10.1073/pnas.91.15.7301	journal	July 1994
Accumulation of D1 polypeptide in tobacco plastids is regulated via the untranslated region of the psbA mRNA. Staub, J. M.; Maliga, P. The EMBO Journal, Vol. 12, Issue 2 https://doi.org/10.1002/j.1460-2075.1993.tb05692.x	journal	February 1993
Inducible Trans-activation of Plastid Transgenes: Expression of the R. eutrophaphb Operon in Transplastomic Tobacco Lössl, Andreas; Bohmert, Karen; Harloff, Hans Plant and Cell Physiology, Vol. 46, Issue 9 https://doi.org/10.1093/pcp/pci157	journal	September 2005
Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic Madison, Lara L.; Huisman, Gjalt W. Microbiology and Molecular Biology Reviews, Vol. 63, Issue 1 https://doi.org/10.1128/MMBR.63.1.21-53.1999	journal	January 1999
Metabolic Engineering: Plastids as Bioreactors Bohmert, Karen; Peoples, Oliver P.; Snell, Kristi D. Molecular Biology and Biotechnology of Plant Organelles https://doi.org/10.1007/978-1-4020-3166-3_21	book	January 2004
Nuclear Genes Encoding the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase Cashmore, A. R. Genetic Engineering of Plants https://doi.org/10.1007/978-1-4684-4544-2_5	book	January 1983
Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants — A review Suriyamongkol, Pornpa; Weselake, Randall; Narine, Suresh Biotechnology Advances, Vol. 25, Issue 2, p. 148-175 https://doi.org/10.1016/j.biotechadv.2006.11.007	journal	March 2007
Construction of a GFP-BAR plasmid and its use for switchgrass transformation Richards, H. A.; Rudas, V. A.; Sun, H. Plant Cell Reports, Vol. 20, Issue 1 https://doi.org/10.1007/s002990000274	journal	January 2001

Similar Records

Genetics and Genomics of Pathogen Resistance in Switchgrass

Technical Report · 2021 · OSTI ID:1783170

Switchgrass biomass and chemical composition for biofuel in eastern Canada

Journal Article · 1999 · Agronomy Journal · OSTI ID:20006692

Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants

Patent · 1997 · OSTI ID:870857

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES

Production of polyhydroxybutyrate in switchgrass

Citation Formats

References (78)

Similar Records

Related Subjects