skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Production of polyhydroxybutyrate in switchgrass

Patent ·
OSTI ID:1176503

Transgenic plants, plant material, and plant cells for synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to a as PHB) are provided. Preferred plants that can be genetically engineered to produce PHB include plants that do not normally produce storage products such as oils and carbohydrates, and plants that have a C.sub.4 NAD-malic enzyme photosynthetic pathway. Such plants also advantageously produce lignocellulosic biomass that can be converted into biofuels. An exemplary plant that can be genetically engineered to produce PHB and produce lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include but are not limited to Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.

Research Organization:
Metabolix, Inc., Cambridge, MA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC07-01ID14214
Assignee:
Metabolix, Inc. (Cambridge, MA)
Patent Number(s):
8,487,159
Application Number:
12/431,428
OSTI ID:
1176503
Resource Relation:
Patent File Date: 2009 Apr 28
Country of Publication:
United States
Language:
English

References (114)

Strcuture and transcription of the nopaline synthase gene region of T-DNA journal January 1983
Modification of the coding sequence enhances plant expression of insect control protein genes. journal April 1991
Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.): significant contents of polyhydroxybutyrate are associated with growth reduction journal April 2003
PHA production, from bacteria to plants journal June 1999
Method for producing transgenic cereal plants patent April 1998
Methods for regulating gene expression patent September 1998
Polyhydroxybutyrate synthesis in transgenic flax journal January 2004
High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. journal February 1993
Proteins with insecticidal properties against homopteran insects and their use in plant protection patent February 1997
Expression of bacterial poly(3-hydroxybutyrate) synthesis genes in hairy roots of sugar beet (Beta vulgaris L.) journal January 2003
Selective gene expression in plants patent May 1992
Method of Agrobacterium-mediated transformation of cultured soybean cells patent October 1996
Engineering of PHB Synthesis Causes Improved Elastic Properties of Flax Fibers journal February 2007
Production of polyhydroxybutyrate in sugarcane journal January 2007
Bonkers about biofuels journal July 2006
Root preferential promoter patent May 1997
Vascular-specific expression of the bean GRP 1.8 gene is negatively regulated. journal October 1991
Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements journal September 1993
Construction of a GFP-BAR plasmid and its use for switchgrass transformation journal January 2001
Tissue-preferential promoters patent November 1995
A novel thiolase-reductase gene fusion promotes the production of polyhydroxybutyrate in Arabidopsis journal July 2005
Alterations in the Chlamydomonas Plastocyanin Transit Peptide Have Distinct Effects on in VitroImport and in Vivo Protein Accumulation journal August 1997
Polyhydroxybutyrate polymerase patent July 1996
Tissue-Specific Activity of Two Manganese Superoxide Dismutase Promoters in Transgenic Tobacco journal October 1996
Automatic SMD tester patent July 1990
Isolation of an efficient actin promoter for use in rice transformation. journal February 1990
Methods of regeneration of Medicago sativa and expressing foreign DNA in same patent June 1994
The chlorophyll a/b-binding protein inserts into the thylakoids independent of its cognate transit peptide. journal October 1988
Method for producing polyester biopolymers patent September 1997
Metabolic Engineering: Plastids as Bioreactors book January 2004
Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids patent November 2001
Protein Trafficking in Plant Cells journal August 1987
Transgenic Arabidopsis plants can accumulate polyhydroxybutyrate to up to 4% of their fresh weight journal November 2000
Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic journal January 1999
Constitutive Expression of the β-Ketothiolase Gene in Transgenic Plants. A Major Obstacle for Obtaining Polyhydroxybutyrate-Producing Plants journal February 2002
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter journal February 1985
Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-  co-3-hydroxyvalerate) copolymer production journal October 1999
Synthetic insecticidal crystal protein gene patent January 1995
Stable transformation of plastids in higher plants. journal November 1990
Tissue-Specific Expression and Promoter Analysis of the Tobacco ltp1 Gene journal October 1996
Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants — A review journal March 2007
Root cortex specific gene promoter patent November 1998
Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase. journal July 1994
Luciferase reporter gene cassettes for plant gene expression studies journal January 1987
CELLULOSIC ETHANOL: Biofuel Researchers Prepare to Reap a New Harvest journal March 2007
A steroid-inducible gene expression system for plant cells. journal December 1991
Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation. journal January 1992
Plant promoter .alpha.-glucuronidase gene construct patent December 1993
Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. journal December 1994
Tissue-Specific and Developmental Regulation of Cotton Gene FbL2A (Demonstration of Promoter Activity in Transgenic Plants) journal November 1996
Chemically inducible expression of the PHB biosynthetic pathway in Arabidopsis journal February 2007
Rice chitinase promoter patent March 1995
Introduction of Bacterial Metabolism into Higher Plants by Polycistronic Transgene Expression journal January 2001
Immunological Characterization and Chloroplast Localization of the Tryptophan Biosynthetic Enzymes of the Flowering Plant Arabidopsis thaliana journal March 1995
Inducible Trans-activation of Plastid Transgenes: Expression of the R. eutrophaphb Operon in Transplastomic Tobacco journal September 2005
-Mediated Genetic Transformation of Switchgrass journal January 2002
Enhanced expression in a plant plastid patent August 1996
Promoter enhancer element for gene expression in plant roots patent June 1991
Expression of Bacillus thuringiensis cry proteins in plant plastids patent August 1996
Plastid Targeting of Polyhydroxybutyrate Biosynthetic Pathway in Tobacco journal January 2001
T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis journal August 1987
Stable Transformation of Soybean Callus by DNA-Coated Gold Particles journal July 1988
Brassica regulatory sequence for root-specific or root-abundant gene expression patent March 1995
Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus. journal January 1991
Genes for the synthesis of antipathogenic substances patent June 1997
Glyphosate-resistant plants patent February 1993
Methods of inserting viral DNA into plant material patent October 1996
Production of a Biodegradable Plastic Polymer, Poly‐β‐Hydroxybutyrate, in Transgenic Alfalfa journal May 2002
Mutations at the Transit Peptide-Mature Protein Junction Separate Two Cleavage Events during Chloroplast Import of the Chlorophyll a/b-binding Protein journal October 1989
Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. journal August 1990
Parameters Influencing Stable Transformation of Rice Immature Embryos and Recovery of Transgenic Plants using Electric Discharge Particle Acceleration journal April 1995
Tissue-specific light-regulated expression directed by the promoter of a C4 gene, maize pyruvate,orthophosphate dikinase, in a C3 plant, rice. journal October 1993
The Biology and Agronomy of Switchgrass for Biofuels journal September 2005
Genetic Transformation of Maize Cells by Particle Bombardment journal September 1989
Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants journal September 1990
Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death: Glucocorticoid-inducible cell death journal April 1998
Plant Regeneraton from Callus Cultures of Switchgrass journal January 1994
Enhanced expression in plants patent January 1997
Identification of a Light-Responsive Region of the Nuclear Gene Encoding the B Subunit of Chloroplast Glyceraldehyde 3-Phosphate Dehydrogenase from Arabidopsis thaliana journal May 1994
Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells journal December 1984
Root specific gene promoter patent October 1995
The Path Forward for Biofuels and Biomaterials journal January 2006
A cassette containing the bar gene of Streptomyces hygroscopicus : a selectable marker for plant transformation journal January 1990
A novel operon organization involving the genes for chorismate synthase (aromatic biosynthesis pathway) and ribosomal GTPase center proteins (L11, L1, L10, L12: rplKAJL) in cyanobacterium Synechocystis PCC 6803. journal December 1993
Soybean transformation by microparticle bombardment patent June 1994
Firefly luciferase gene: structure and expression in mammalian cells journal February 1987
Metabolic pathway engineering in cotton: Biosynthesis of polyhydroxybutyrate in fiber cells journal November 1996
Molecular breeding of switchgrass for use as a biofuel crop journal December 2007
Tetracycline-regulated transcriptional inhibitors patent August 1998
Light-responsive elements of the tobacco PSI-D gene are located both upstream and within the transcribed region journal August 1997
Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker for site-directed transformation of chlamydomonas journal January 1991
Poly(β-hydroxybutyrate) production in oilseed leukoplasts of Brassica napus journal October 1999
Insecticidal cotton plants patent March 1997
Plant group 2 promoters and uses thereof patent March 1997
Net energy of cellulosic ethanol from switchgrass journal January 2008
Multigene expression vectors for the biosynthesis of products via multienzyme biological pathways patent September 2002
Particle gun patent August 1993
Method for transporting substances into living cells and tissues and apparatus therefor patent July 1990
Chemically inducible expression of biosynthetic pathways patent June 2010
Transgenic maize plants by tissue electroporation. journal December 1992
Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16 journal September 1989
ALS3 promoter patent August 1997
Synthetic promoters patent June 2000
Signal peptide analogs derived from two chloroplast precursors interact with the signal recognition system of the chloroplast envelope. journal February 1991
Switchgrass as a biofuels feedstock in the USA journal December 2006
Transfer of foreign genes into intact maize cells with high-velocity microprojectiles journal June 1988
Production of Polyhydroxybutyrate by Polycistronic Expression of Bacterial Genes in Tobacco Plastid journal September 2004
Pathogen-resistant transgenic plants patent May 1998
Spatio-temporal characterization of polyhydroxybutyrate accumulation in sugarcane journal January 2007
Analysis of three tissue-specific elements from the wheat Cab-1 enhancer: Functional elements of a Cab enhancer journal April 1993
Method for stably transforming plastids of multicellular plants patent September 1995
Mannose or xylose based positive selection patent June 1998
Stable transformation of tobacco by electroporation: evidence for plasmid concatenation. journal August 1986
Accumulation of D1 polypeptide in tobacco plastids is regulated via the untranslated region of the psbA mRNA. journal February 1993