High density growth of T7 expression strains with auto-induction option

Studier, F. William

High density growth of T7 expression strains with auto-induction option

Patent · Tue Apr 27 04:00:00 EDT 2010

OSTI ID:1176255

Studier, F. William

Methods for promoting and suppressing auto-induction of transcription of cloned DNA in cultures of T7 expression strains are disclosed.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-98CH10886

Assignee:: Brookhaven Science Associates, LLC (Upton, NY)

Patent Number(s):: 7,704,722

Application Number:: 11/741,048

OSTI ID:: 1176255

Country of Publication:: United States

Language:: English

References (13)

Stability of plasmid and expression of a recombinant gonadotropin-releasing hormone (GnRH) vaccine in Escherichia coli Xu, Jinshu; Li, Wenjia; Wu, Jie Applied Microbiology and Biotechnology, Vol. 73, Issue 4 https://doi.org/10.1007/s00253-006-0547-7	journal	December 2006
The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep Grabski, Anthony; Mehler, Mark; Drott, Don Nature Methods, Vol. 2, Issue 3 https://doi.org/10.1038/nmeth0305-233	journal	March 2005
The General Stress Sigma Factor ςS ofEscherichia coli Is Induced during Diauxic Shift from Glucose to Lactose Fischer, Daniela; Teich, Antje; Neubauer, Peter Journal of Bacteriology, Vol. 180, Issue 23 https://doi.org/10.1128/.180.23.6203-6206.1998	journal	January 1998
[6] Use of T7 RNA polymerase to direct expression of cloned genes William Studier, F.; Rosenberg, Alan H.; Dunn, John J. Methods in Enzymology https://doi.org/10.1016/0076-6879(90)85008-C	book	January 1990
Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability Grossman, Trudy H.; Kawasaki, Ernest S.; Punreddy, Sandhya R. Gene, Vol. 209, Issue 1-2 https://doi.org/10.1016/S0378-1119(98)00020-1	journal	March 1998
Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor Dubendorf, John W.; Studier, F. William Journal of Molecular Biology, Vol. 219, Issue 1 https://doi.org/10.1016/0022-2836(91)90856-2	journal	May 1991
Fermentation conditions for high-level expression of the tac -promoter-controlled calf prochymosin cDNA in Escherichia coli HB101: FERMENTATION CONDITIONS FOR HIGH-LEVEL EXPRESSION Kaprálek, F.; Ječmen, P.; Sedláček, J. Biotechnology and Bioengineering, Vol. 37, Issue 1 https://doi.org/10.1002/bit.260370111	journal	January 1991
cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli Kimata, K.; Takahashi, H.; Inada, T. Proceedings of the National Academy of Sciences, Vol. 94, Issue 24 https://doi.org/10.1073/pnas.94.24.12914	journal	November 1997
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes Studier, F. William; Moffatt, Barbara A. Journal of Molecular Biology, Vol. 189, Issue 1, p. 113-130 https://doi.org/10.1016/0022-2836(86)90385-2	journal	May 1986
Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination Tyler, Robert C.; Sreenath, Hassan K.; Singh, Shanteri Protein Expression and Purification, Vol. 40, Issue 2 https://doi.org/10.1016/j.pep.2004.12.024	journal	April 2005
Protein production by auto-induction in high-density shaking cultures Studier, F. William Protein Expression and Purification, Vol. 41, Issue 1, p. 207-234 https://doi.org/10.1016/j.pep.2005.01.016	journal	May 2005
Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium Sreenath, Hassan K.; Bingman, Craig A.; Buchan, Blake W. Protein Expression and Purification, Vol. 40, Issue 2 https://doi.org/10.1016/j.pep.2004.12.022	journal	April 2005
Protein production in Escherichia coli for structural studies by X-ray crystallography Goulding, Celia W.; Jeanne Perry, L. Journal of Structural Biology, Vol. 142, Issue 1 https://doi.org/10.1016/S1047-8477(03)00044-3	journal	April 2003

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