High density growth of T7 expression strains with auto-induction option

Studier, F William

Title: High density growth of T7 expression strains with auto-induction option

Patent · Tue Jul 20 00:00:00 EDT 2010

OSTI ID:1015637

Studier, F William ^[1]

Stony Brook, NY

A bacterial growth medium for promoting auto-induction of transcription of cloned DNA in cultures of bacterial cells grown batchwise is disclosed. The transcription is under the control of a lac repressor. Also disclosed is a bacterial growth medium for improving the production of a selenomethionine-containing protein or polypeptide in a bacterial cell, the protein or polypeptide being produced by recombinant DNA techniques from a lac or T7lac promoter, the bacterial cell encoding a vitamin B12-dependent homocysteine methylase. Finally, disclosed is a bacterial growth medium for suppressing auto-induction of expression in cultures of bacterial cells grown batchwise, said transcription being under the control of lac repressor.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-98CH10886

Assignee:: Brookhaven Science Associates, LLC (Upton, NY)

Patent Number(s):: 7,759,109

Application Number:: 12/062,565

OSTI ID:: 1015637

Country of Publication:: United States

Language:: English

References (19)

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